Simple LC–MS Determination of Citric and Malic Acids in Fruits and Vegetables

Fernández-Fernández, Remedios; López-Martínez, Carlos; Romero‐González, Roberto; Martı́nez-Vidal, José Luis; Flores, María Isabel Alarcón; Frenich, Antonia Garrido

doi:10.1365/s10337-010-1611-0

Cited by 58 publications

(24 citation statements)

References 29 publications

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“…Organic acids in SLM were identified and measured using a Waters Xevo G2-XS QTOF mass spectrometer (Waters Corporation, Milford, MA USA) coupled with a Waters Acquity H Class chromatography system. Organic acids were separated on a Waters Acquity UPLC HSS C18 1.8 μm 2.1 × 100 mm column using a modification of a previously published method (Fernández-Fernández et al, 2010 ). Mobile phases were methanol (solvent A) and water with 0.5% formic acid (solvent B).…”

Section: Methodsmentioning

confidence: 99%

Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149

et al. 2018

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Highly concentrated radionuclide waste produced during the Cold War era is stored at US Department of Energy (DOE) production sites. This radioactive waste was often highly acidic and mixed with heavy metals, and has been leaking into the environment since the 1950s. Because of the danger and expense of cleanup of such radioactive sites by physicochemical processes, in situ bioremediation methods are being developed for cleanup of contaminated ground and groundwater. To date, the most developed microbial treatment proposed for high-level radioactive sites employs the radiation-resistant bacterium Deinococcus radiodurans. However, the use of Deinococcus spp. and other bacteria is limited by their sensitivity to low pH. We report the characterization of 27 diverse environmental yeasts for their resistance to ionizing radiation (chronic and acute), heavy metals, pH minima, temperature maxima and optima, and their ability to form biofilms. Remarkably, many yeasts are extremely resistant to ionizing radiation and heavy metals. They also excrete carboxylic acids and are exceptionally tolerant to low pH. A special focus is placed on Rhodotorula taiwanensis MD1149, which was the most resistant to acid and gamma radiation. MD1149 is capable of growing under 66 Gy/h at pH 2.3 and in the presence of high concentrations of mercury and chromium compounds, and forming biofilms under high-level chronic radiation and low pH. We present the whole genome sequence and annotation of R. taiwanensis strain MD1149, with a comparison to other Rhodotorula species. This survey elevates yeasts to the frontier of biology's most radiation-resistant representatives, presenting a strong rationale for a role of fungi in bioremediation of acidic radioactive waste sites.

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Section: Methodsmentioning

confidence: 99%

Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149

et al. 2018

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“…Many methods have been developed for the determination of low molecular mass organic acids in a wide variety of complex matrices, including fruits (Chinnici, Spinabelli, Riponi, & Amati, 2005;Fernández-Fernández et al, 2010;Hernandez, Lobo, & Gonzalez, 2009;Mato, Suárez-Luque, & Huidobro, 2007). Spectrophotometric and enzymatic methods have been used for the determination of some organic acids (Mato, Suárez-Luque, & Huidobro, 2005); however these techniques are tedious and time-consuming since determine each acid individually and cannot be used to analyze the whole range of acids present in the sample.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid and direct method for the determination of organic acids in peach fruit using LC–ESI-MS

et al. 2016

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“…Compounds P1 aliphatic and P5 were identified by the in-house database as malic acid and caffeic acid; with pseudomolecular ion peaks [M-H] − at m/z 133.0135 and 179.0577, respectively 38,39 .…”

Section: Identification Of Phenolic Acids Identified Phenolic Acids mentioning

confidence: 99%

LC–MS/MS and GC–MS profiling as well as the antimicrobial effect of leaves of selected Yucca species introduced to Egypt

Sayed

Basam

El-Naggar

et al. 2020

Sci Rep

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Few studies thoroughly investigated different Yucca species introduced to Egypt. As a part of our ongoing investigation of the Yucca species; Yucca aloifolia and its variety Yucca aloifolia variegata, Yucca filamentosa, and Yucca elephantipes (Asparagaceae) were extensively subjected to phytochemical and antimicrobial investigation. Yucca species cultivated in Egypt showed no antimicrobial effect. GC/MS of the lipoid contents of Y. aloifolia variegata was carried out. Twenty-six fatty acids were identified. Saturated fatty acids established almost twice the unsaturated ones and constituted 64.64% of which palmitic acid and palmitoleic acid signifying 58.28% and 30.98%, respectively. Hydrocarbons were 21 constituting 39.64% of the unsaponifiable fraction. Only three sterols 42.36% were detected, major was γ-sitosterol. LC–MS/MS comparison of the 4 plant extracts imply that Y.aloifolia variegata L extract was the richest, which was apparent through its superior biological activity. LC–MS/MS analysis of the total alcoholic extract (Alc) of the leaves of Y.aloifolia variegata L. was performed using MS-techniques at different voltages; equal to 35 and 135 eV. Negative and positive-ion modes analyses at low fragmentation energy allowed the tentative identification of 41 and 34 compounds, respectively. The LC–ESI–MS/MS analysis in the positive mode proved to be better in the identification of saponins.

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Simple LC–MS Determination of Citric and Malic Acids in Fruits and Vegetables

Cited by 58 publications

References 29 publications

Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149

Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149

Development of a rapid and direct method for the determination of organic acids in peach fruit using LC–ESI-MS

LC–MS/MS and GC–MS profiling as well as the antimicrobial effect of leaves of selected Yucca species introduced to Egypt

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