Development of a rapid and direct method for the determination of organic acids in peach fruit using LC–ESI-MS

Iridium complexes catalysed the selective dehydrogenation of glucose to gluconic acid in water

“…Details of the method optimization and chromatographic conditions for quantitation purposes are given in the ESI. † 29,30 The HPLC-MS/MS method consists of taking small aliquots of the crude reaction mixture, diluting them with water/methanol (95 : 5) and directly injecting them into the HPLC-MS system. This methodology allows to (i) monitor glucose and gluconic acid without unnecessary work-up, minimizing the number of errors from sample pre-treatment steps, and (ii) obtain detailed temporal reaction profiles and rapid reaction optimization.…”

Potential prebiotic properties of cashew apple (Anacardium occidentale L.) agro‐industrial byproduct on Lactobacillus species

“…Citric acid and malic acid are organic acids naturally present in fruit pulps and their byproducts, although some lactic acid bacteria are capable of producing them under different fermentation conditions . Otherwise, lactic and acetic acids are common end‐products of lactic acid bacteria fermentation, and the production of these acids in higher amounts during the growth of probiotics is directly associated with intense metabolic activities.…”

Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages

“…During trials of various homogenization procedures, it was observed that complete dissolution of organic acids in the extraction solvents occurred when the homogenizer was set at 5000 rpm for 10 min. It should also be taken into account that the homogenization procedure might have raised the temperature of the solution, potentially resulting in the decomposition of the analytes . So, the sample was placed in ice every 150 s. In general, sample pretreatment procedures include extraction and purification.…”

“…Therefore, the highest content of organic acids in the TCA cycle occurred in the exponential phase. The exception was oxaloacetic acid, which was easily hydrolyzed to pyruvate because of its instability at acidic and basic pH or high temperature . The stationary phase brought about a decreased rate of photosynthesis and enzymatic activity.…”