“…Duplicate injections were performed, and the average peak areas were used for quantification. 16 Tartaric, malic, lactic, citric, succinic, and acetic acids were detected by the DAD at 210 nm; glucose and fructose were detected by the RID. The standards for glucose and fructose were obtained from Sigma-Aldrich (St Louis, USA), and for citric, acetic, lactic, tartaric, and malic acids they were obtained from Vetec Química Fina (Rio de Janeiro, Brazil), all with a purity of ≥ 99%.…”