Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits

Ribeiro, Francisco W.P.; Barroso, M. Fátima; Morais, Simone; Subramanian, Venkataraman; Lima‐Neto, Pedro de; Correia, Adriana N.; Oliveira, M. Beatriz P.P.; Delerue–Matos, Cristina

doi:10.1016/j.bioelechem.2013.09.005

Cited by 48 publications

(16 citation statements)

References 43 publications

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“…The results achieved with the proposed biosensor are in agreement or compare favorably with those reported previously (scarce and mainly based on acetylcholinesterase) for CBR [54][55][56][57], PPX [56], FMT [10,13] and ZRM [13,58].…”

Section: Electroanalytical Characteristicssupporting

confidence: 79%

“…In this perspective, the biosensing of environmental pollutants, particularly agrochemicals, using enzymes as biorecognition element has increased pronouncedly in the last years [1,[9][10][11][12][13][14]. Still, many of these devices need to improve their performance because of the low maximum residue limits (MRLs) established worldwide for pesticides [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Oliveira

Barroso

Araújo

et al. 2014

Bioelectrochemistry

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A bi-enzymatic biosensor (LACC-TYR-AuNPs-CS/GPE) for carbamates was prepared in a single step by electro-deposition of a hybrid film onto a graphene doped carbon paste electrode (GPE). Graphene and the gold nanopar-ticles (AuNPs) were morphologically characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, dynamic light scattering and laser Doppler velocimetry. The electrodeposited hybrid film was composed of laccase (LACC), tyrosinase (TYR) and AuNPs entrapped in a chitosan (CS) polymeric matrix. Exper-imental parameters, namely graphene redox state, AuNPs:CS ratio, enzymes concentration, pH and inhibition time were evaluated. LACC-TYR-AuNPs-CS/GPE exhibited an improved Michaelis-Menten kinetic constant (26.9 ± 0.5 M) when compared with LACC-AuNPs-CS/GPE (37.8 ± 0.2 M) and TYR-AuNPs-CS/GPE (52.3 ± 0.4 M). Using 4-aminophenol as substrate at pH 5.5, the device presented wide linear ranges, low detection limits (1.68 × 10 −9 ± 1.18× 10 −10 -2.15 × 10 −7 ± 3.41 × 10 −9 M), high accuracy, sensitivity (1.13 × 10 6 ± 8.11 × 10 4 -2.19 × 10 8 ± 2.51× 10 7 %inhibition M −1 ), repeatability (1.2-5.8% RSD), reproducibility (3.2-6.5% RSD) and stability (ca. twenty days) to determine carbaryl, formetanate hydrochloride, propoxur and ziram in citrus fruits based on their inhibitory capacity on the polyphenoloxidases activity. Recoveries at two fortified levels ranged from 93.8 ± 0.3% (lemon) to 97.8 ± 0.3% (orange). Glucose, citric acid and ascorbic acid do not interfere signifi-cantly in the electroanalysis. The proposed electroanalytical procedure can be a promising tool for food safety control.

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Section: Electroanalytical Characteristicssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Oliveira

Barroso

Araújo

et al. 2014

Bioelectrochemistry

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“…The working mechanism of the biosensor is based on the formethanate hydrochloride inhibition of the laccase catalytic reaction that takes place in the presence of phenolic compounds. The laccase-based biosensor which was developed provided a good performance for monitoring formethanate from real samples, grapes and mango [ 85 ].…”

Section: Other Enzymesmentioning

confidence: 99%

Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more “biologically relevant” than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides.

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“…In comparison with other electroanalytical methods for FMT sensing, the sensor proposed in this paper obtained comparable results to a sensor composed by cobalt phthalocyanine and funcionalized multi walled carbon nanotube modified glassy carbon electrode [40] which showed a wide linear range from 9.80 × 10 −8 to 3.92 × 10 −6 mol L −1 and DL value equal to 9.70 × 10 −8 mol L −1 , a bi-enzymatic biosensor composed for laccase, tyrosinase and AuNPs entrapped in a chitosan in a graphene doped carbon paste electrode s [41] with linear range from 9.99 × 10 −7 to 3.21 × 10 −5 mol L −1 with DL value equal to 2.15 × 10 −7 mol L −1 and a enzymatic biosensor composed by laccase, glutaraldehyde onto a gold electrode s [42] with present a linear range from 9.43 × 10 −7 to 1.13 × 10 −5 mol L −1 with DL value equal to 9.5 × 10 −8 mol L −1 . The proposed sensor presents a similar linear range with others described in the literature, and DL in the same order with a biosensor, described, which is one construction more expensive and complex.…”

Section: Electroanalytical Curvesmentioning

confidence: 99%

“…FMT is a carbamate pesticide miticide/insecticide authorized to be used on several fruits and nonagricultural uncultivated areas/soils [37,38]. Its quantification was rarely performed by electrochemical techniques, being only made by the conventional hanging mercury drop electrode [39], and more recently with the glassy carbon modified with cobalt phthalocyanine and functionalized multiwalled carbon nanotubes [40], and also by three different laccase-based biosensors electrochemical biosensor [14,41,42]. Thus, the main goal of this study was to characterize the electrochemical behavior of FMT on BDDE (cathodically pretreated) and subsequently optimize a simple, sensitive, accurate and inexpensive electroanalytical procedure for FMT sensing in fruits.…”

Section: Introductionmentioning

confidence: 99%

Sensing of formetanate pesticide in fruits with a boron-doped diamond electrode

Ribeiro

Sousa

Morais

et al. 2018

Microchemical Journal

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This study describes the development of a simple and accurate methodology for carbamate pesticide formetanate (FMT) analysis in fruits based on the use of a boron-diamond doped electrode (BDDE) cathodically pretreated and on the forward component of the current of square-wave voltammetry (SWV). FMT exhibits a well-defined irreversible oxidation process, which reaction mechanism is diffusion-controlled, involves the participation of one electron and is influenced by the electrolyte pH. However, protonation does not participate in the ratedetermining step in the redox process. The optimum experimental and voltammetric conditions were pH 7.0 (0.04 mol L −1 Britton-Robinson buffer), pulse potential frequency of 20 s −1 , amplitude of the pulse of 25 mV, and height of the potential step of 3 mV. Under the optimum conditions, calibration curve was linear from 4.98 × 10 −7 to 1.70 × 10 −5 mol L −1 FMT with a limit of detection of 3.7 × 10 −7 mol L −1. FMT sensing was performed in different fruits (mango and grape). Recoveries ranged from 95.2 ± 2.8 to 104.0 ± 3.5% for mango and 96.5 ± 2.5 to 105.2 ± 3.5% for grape proving the accuracy and precision of the electroanalytical methodology. The attained data validated the applicability of the developed approach for FMT quantification in fruits.

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Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits

Cited by 48 publications

References 43 publications

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Advances in Enzyme-Based Biosensors for Pesticide Detection

Sensing of formetanate pesticide in fruits with a boron-doped diamond electrode

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