Simple high-performance liquid chromatographic method for the determination of medroxyprogesterone acetate in human plasma

Application of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma

“…Numerous recent researches for this analysis have been reported concerning the method sensitivity and selectivity in association with various instruments including GC, HPLC and others [6][7][8][9][10][11][12][13][14][15]. However, each of which had their advantages and disadvantages.…”

“…Attempts have been made to optimize the methods for MPA analysis using small amounts of plasma samples [7,8]. Quantitative analysis of MPA in plasma by HPLC with ultraviolet (UV) detection was also carried out, but did not give satisfactory detection limit [9,10]. The high sensitivity of the GC-MS has also been used to monitor the MPA level in human serum [11].…”

A stability-indicating HPLC method for medroxyprogesterone acetate in bulk drug and injection formulation

“…Up to now, several methods including spectrophotometry [4,5], HPLC [6][7][8][9], LC-MS [10][11][12][13][14][15][16], GC-MS [16][17][18][19][20] and immunoassay [21] have been published for determining MPA in pharmaceutical preparations and biological fluids. Two stabilityindicating HPLC methods for MPA tablet formulations have been reported.…”

Simultaneous Determination of Estradiol Valerate and Medroxyprogesterone Acetate in a Tablet Formulation by Gas Chromatography-Mass Spectrometry

“…2,3 The combination estrogen-gestagen is used for the treatment of estrogenic deficiency syndrome to control its symptoms (climateric syndrome), such as loss of bone minerals and development of heart disease. 4 Up to now, various analytical methods for the determination of EV or MPA have been reported including voltammetry, 1 spectrophotometry, 5,6 fluorometry, 7 high performance liquid chromatography (HPLC), 4,[8][9][10][11] gas chromatography-mass spectrometry (GC-MS), [12][13][14] FT-Raman spectroscopy 15 and chemiluminescence enzyme immunoassay 16 in pharmaceutical preparations and biological liquids. But there has been no GC-MS method reported in the litearature for simultaneous determination of these two hormones in pharmaceutical preparations.…”