A stability-indicating HPLC method for medroxyprogesterone acetate in bulk drug and injection formulation

An enzyme-linked immunoassay for the detection of medroxyprogesterone acetate in intestines based on monoclonal antibody

“…Several methods have been developed to determine MPA, such as spectrophotometry (De Beer et al, 2004), high-performance liquid chromatography (Burana-Osot, Ungboriboonpisal, & Sriphong, 2006;Fatmi, Williams, & Hickson, 1988;Segall et al, 1999), liquid chromatography mass spectroscopy (Hooijerink et al, 2003;Rambaud et al, 2005), gas chromatography with mass spectrometry detection (GC/MS) (Choi et al, 2001;Daeseleire, De Guesquière, & Van Peteghem, 1991) and so on. Among the reported methods, GC/MS is the most commonly applied detection technique.…”

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

“…These stability-indicating methods are almost exclusively RP-HPLC methods with UV detection. The determination of betamethasone [67], betamethasone dipropionate [68], budesonide [69], clobetasol propionate [70], deflazacort [71], dexamethasone [71][72][73][74], dexamethasone acetate [75], dexamethasone sodium phosphate [72,76], diflucortolone valerate [77], dutasteride [19], eplerenone [18], estradiol [21,78,79], ethinylestradiol [80], fluocinolone acetonide [81], finasteride [82], fluticasone propionate [83], gestodene [80], medroxyprogesterone acetate [14], meprednisone [84], mometasone furoate [85], testosterone propionate and cypionate [86] and triamcinolone acetonide [87] in various solid, semi-solid and liquid formulations have been described. The on-line assay of beclomethasone propionate pressurized metered dose inhalers [88] and the determination of the solubility of beclomethasone propionate [89] and budesonide [90] in the same device are also worth mentioning.…”

“…Other stability indicating HPLC methods were published for the assay of budesonide [13], medroxyprogesterone acetate [14], loteprednol etabonate [15], examestane [16], etiprednol dicloacetate [17], eplerenone [18], dutasteride [19,20], estradiol [21], betamethasone [22] and intermediates in steroid syntheses such as alphamethylepoxide (16␣-methyl-1,4-pregnadiene-9␤,11␤-oxide-17␣,21-diol-3,20-dione) [23] and its 16␤-methyl analogue, betamethylepoxide [24].…”

“…An example for this is the monograph of fulvestrant in USP 33 [9] where these data for five potential impurities are presented. This approach can be found in the papers already mentioned in connection with the stability-indicating assays [13][14][15][16][17][18][19][20][21][22][23][24].…”

Simultaneous Determination of Estradiol Valerate and Medroxyprogesterone Acetate in a Tablet Formulation by Gas Chromatography-Mass Spectrometry

“…2,3 The combination estrogen-gestagen is used for the treatment of estrogenic deficiency syndrome to control its symptoms (climateric syndrome), such as loss of bone minerals and development of heart disease. 4 Up to now, various analytical methods for the determination of EV or MPA have been reported including voltammetry, 1 spectrophotometry, 5,6 fluorometry, 7 high performance liquid chromatography (HPLC), 4,[8][9][10][11] gas chromatography-mass spectrometry (GC-MS), [12][13][14] FT-Raman spectroscopy 15 and chemiluminescence enzyme immunoassay 16 in pharmaceutical preparations and biological liquids. But there has been no GC-MS method reported in the litearature for simultaneous determination of these two hormones in pharmaceutical preparations.…”