Simple high-performance liquid chromatographic method with electrochemical detection for the simultaneous determination of artesunate and dihydroartemisinin in biological fluids

Validation of high performance liquid chromatography–electrochemical detection methods with simultaneous extraction procedure for the determination of artesunate, dihydroartemisinin, amodiaquine and desethylamodiaquine in human plasma for application in clinical pharmacological studies of artesunate–amodiaquine drug combination

“…25 • C) using Reacti-Vap TM 18780 drying system (Pierce, Rockford, IL, USA). The dried extracts containing AS, DHA and QHS were reconstituted with 100 l of ethanol-water (1:1, v/v) and kept at 4 • C for at least 18 h before analysis to ensure that the tautomerization of ␣ and ␤ isomers of DHA reaches equilibrium [4,12,13,16]. Dried extracts of AQ, DeAQ and IB-DeAQ were reconstituted with 100 l of 0.01 M HCl.…”

“…HPLC with electrochemical detection (EC) remains the standard as it is affordable and sensitive enough for pharmacokinetic studies. However, the existing HPLC-EC methods for the quantification of AS [12,13] and AQ [14,15] ing AS, AQ and their metabolites simultaneously. Hence, this article reports a validated analytical method which allows simultaneous extraction of AS, AQ and their metabolites from human plasma, followed by the determination of these analytes using HPLC-EC systems.…”

A liquid chromatographic-tandem mass spectrometric method for determination of artesunate and its metabolite dihydroartemisinin in human plasma

“…Several publications on the determination of artemisinin and its derivatives using ECD have been published [12][13][14]. The limit of detection for these methods is around 5-20 ng/ml using 0.5-1 ml plasma.…”

Pharmacokinetics of dihydroartemisinin in Artekin tablets for single and repeated dosing in Chinese healthy volunteers

“…Due to the absence of appropriate UV absorption of the DHA structure, high performance liquid chromatography (HPLC) with postcolumn alkaline or acidic derivatization for UV detection are popular techniques [7][8][9][10]. The presence of a peroxide bridge in the structure of DHA offers the advantage of using HPLC with reductive electrochemical detection [11][12][13]. HPLC with mass spectrometry (LC-MS) for the assay of DHA [14][15][16][17][18] was recently reported.…”