Simple Fluorescence Sensing Approach for Selective Detection of Fe³⁺ Ions: Live-Cell Imaging and Logic Gate Functioning

Abstract

A pyrene-based fluorescent chemosensor APSB [ N -(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe 3+ in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe 3+ ion detection. The detection limit for APSB was 1.95 nM, and the binding constant ( K b ) was obtained as 8.20 × 10 5 M –1 in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB–Fe 3+ were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe 3+ in living cells.

Synthesis of a novel hydrazone-based compound applied as a fluorescence turn-on chemosensor for iron(iii) and a colorimetric sensor for copper(ii) with antimicrobial, DFT and molecular docking studies

“…The frontier molecular orbitals provided effective information about electronic transitions, reactivity, biological activity, and kinetic stability of L as well as metal complexes of Fe 3+ and Cu 2+ . 40–43 The highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) of L were located within mostly on the whole π-moiety of thiophene as well as the –CO–NHNCH– moiety with a HOMO–LUMO gap of 2.507 eV, which indicated the transition of π to π* of the thiophene unit. As evident from UV-visible and fluorescence titration spectra, the stoichiometry of L with Fe 3+ and Cu 2+ were confirmed strongly as 2 : 1 and hence, the optimization of the metal complex was carried out with 2 : 1 coordinated complexes.…”

Pilot Study on the Visualization of Latent Fingerprints and Naked Eye Detection of Hg2+ and Zn2+ Ions in Aqueous Media Using Ninhydrin-Based Thiosemicarbazone

“…0.604 and 0.494 μM were discovered to be the detection thresholds for Hg 2+ and Zn 2+ , respectively. The plot of 1/( I – I 0 ) vs 1/[M] was used to obtain the K a value …”

“…The plot of 1/(I − I 0 ) vs 1/ [M] was used to obtain the K a value. 25 Latent Fingerprint Impression and Development Methods. Many powdered compounds have been used for the development of fingerprints that can help forensic scientists to lift fingerprints from crime scenes and also the defense scientists for identifying deceased soldiers.…”

Exploring the anticancer potential of thiadiazole derivatives of substituted thiosemicarbazones formed via copper‐mediated cyclization

“…Equimolar amounts of fluorene-2-carboxaldehyde (0.1942 g, 1 mmol) and pyrrolidine-1-carbothiohydrazide (0.1452 g, 1 mmol) were dissolved in ethanol (20 mL), and the mixture was refluxed in the presence of few drops of glacial acetic acid at 60-70 C for 6 h. 40,41 A yellow precipitate of FP was observed in the reaction mixture. TLC was used at the proper intervals to validate the product's formation.…”