Simple Coatings to Render Polystyrene Protein Resistant

Abstract

Non-specific protein adsorption is detrimental to the performance of many biomedical devices. Polystyrene is a commonly used material in devices and thin films. Simple reliable surface modification of polystyrene to render it protein resistant is desired in particular for device fabrication and orthogonal functionalisation schemes. This report details modifications carried out on a polystyrene surface to prevent protein adsorption. The trialed surfaces included Pluronic F127 and PLL-g-PEG, adsorbed on polystyrene, using a polydopamine-assisted approach. Quartz crystal microbalance with dissipation (QCM-D) results showed only short-term anti-fouling success of the polystyrene surface modified with F127, and the subsequent failure of the polydopamine intermediary layer in improving its stability. In stark contrast, QCM-D analysis proved the success of the polydopamine assisted PLL-g-PEG coating in preventing bovine serum albumin adsorption. This modified surface is equally as protein-rejecting after 24 h in buffer, and thus a promising simple coating for long term protein rejection of polystyrene.

Electrically Conductive Polydopamine–Polypyrrole as High Performance Biomaterials for Cell Stimulation in Vitro and Electrical Signal Recording in Vivo

“…Interestingly, more protein adsorption was found from the PDA/PPY substrates than the PPY substrates (typically1.5–3.4 times higher for PDA/PPY than PPY substrates prepared with the same deposition charges). These QCM experimental results suggest that the dopamine and PDA in the PDA/PPY copolymer enhances serum protein adsorption and thereby supports cell growth and differentiation, which is consistent with the findings of previous studies that PDA can encourage protein adsorption and thus cell adhesion. − …”

“…Comparison of cell growth on PPY and PDA/PPY substrates, which had with similar surface roughness, demonstrated that the PDA/PPY is superior to PPY in supporting cell growth. For example, PDA/ PPY (200 mC) and PDA/PPY (300 mC) showed better adhesion of myoblasts and neuronal cells compared to PPY (100 mC) although they have a similar surface roughness (implying that poor adhesion on PPY did not result from 50,64 Not only electrically excitable cells (e.g., neuronal cells and muscle cells) but also other cells (e.g., osteoblasts and stem cells) are reported to respond to electrical stimulation. In this study, electrical stimulation of PC12 cells was performed to examine possible promotion of neurite outgrowth for the demonstration of uses of the PDA/PPY biomaterials as scaffolds that are able to effectively modulate cellular responses by electrical stimulation.…”

Electrochemical Co-deposition of Polydopamine/Hyaluronic Acid for Anti-biofouling Bioelectrodes

“…For the PDA (60 min)-modified samples, which showed the highest protein adsorption, the R ct increased by approximately 43 times post incubation compared with that of PDA/HA. These results are consistent with previous studies that PDA promotes protein adsorption (Hecker et al, 2018). The resistance changes of the PDA/HA (60 min)-modified electrode was relatively small compared with those of the other samples.…”

The Effect of Dynamic, In Vivo-like Oxaliplatin on HCT116 Spheroids in a Cancer-on-Chip Model Is Representative of the Response in Xenografts

“…For ensuring non-adhesion of the spheroids to the well, a coating of Pluronic F127 was used [ 21 ]. Pluronics attach to the hydrophobic PDMS with a central hydrophobic block, and the hydrophilic tails form a hydrophilic brush which prevents binding of proteins and cells [ 21 , 22 ]. Pluronics have been shown to prevent cell adhesion for up to 4 weeks, and they remain intact in microfluidic channels after flow [ 23 , 24 ].…”