Simple coating of capillaries with anionic liposomes in capillary electrophoresis

Hautala, Jari T.; Lindén, Maria V.; Wiedmer, Susanne Κ.; Ryhänen, Samppa J.; Säily, Matti; Kinnunen, Paavo K.J.; Riekkola, Marja‐Liisa

doi:10.1016/s0021-9673(03)00570-3

Cited by 71 publications

(74 citation statements)

References 41 publications

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“…The emphasis was on the rigidity and stability of the phospholipid coating. In a previous work, we have described a simple method for coating a fused-silica capillary with phospholipids [1] and showed how temperature and addition of divalent cations [3] or the surfactant CTAB [29] affect the rigidity of the phospholipid phase and the stability of the coating for CE.…”

Section: Resultsmentioning

confidence: 99%

“…The mixture was evaporated to dryness under a stream of nitrogen, and traces of solvent were removed by evacuation under reduced pressure for ,16 h. The lipid residues were hydrated in 40 mM HEPES pH 7.4 at 607C to yield multilamellar vesicles (MLVs) with a lipid concentration of 0.75-1 mM, and the vesicles were maintained at this temperature for 60 min with subsequent vortexing. The resulting dispersion was processed to LUVs by extrusion 19 times through Millipore 0.1 mm pore size polycarbonate filters with a LiposoFast extruder, essentially as described previously [1,28]. The liposome dispersions containing DPPC and SM were extruded at 607C, since the T m 's of DPPC and SM are close to 407C and the liposome dispersion has to be in fluid state during extrusion.…”

Section: Liposome Preparationmentioning

confidence: 99%

“…Liposomes are attracting considerable interest as a coating material in CE [1][2][3][4][5]. The simple and fast method of preparing the semipermanent phospholipid coating, and its versatility, make CE a promising technique for studies on phospholipid-analyte interactions.…”

Section: Introductionmentioning

confidence: 99%

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Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

et al. 2006

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The purpose was to develop a stable biological membrane coating for CE useful for membrane interaction studies. The effect of cholesterol (chol) on the stability of dipalmitoylphosphatidylcholine (DPPC) and sphingomyelin (SM) coatings was studied. In addition, a fused-silica capillary for CE was coated with human red blood cell (RBC) ghost lipids. Liposomes prepared of DPPC/SM with and without chol or RBC ghost lipids were flushed through the capillary and the stability of the coating was measured electrophoretically. Similar mixtures of DPPC/SM with and without chol were further studied by differential scanning calorimetry. The presence of phosphatidylcholine as a basic component in the coating solution of DPPC/SM/chol was found to be essential to achieve a good and stable coating. The results also confirmed the stability of coatings obtained with solutions of DPPC with 0-30 mol% of chol and SM in different ratios, which more closely resemble natural membranes. Finally, the electrophoretic measurements revealed that a stable coating is formed when capillaries are coated with liposomes of RBC ghost lipids.

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Section: Resultsmentioning

confidence: 99%

Section: Liposome Preparationmentioning

confidence: 99%

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Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

et al. 2006

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“…In earlier capillary electrophoretic (CE) studies, we have used liposomes both as a dispersed phase [11][12][13] and as a capillary coating material [14,15]. A simple method for coating of fused-silica capillaries with anionic liposomes was reported, and the coated capillaries were used for the separation of uncharged analytes in CE (capillary electrochromatography, CEC) [15]. N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) (HEPES) used as BGE was shown to play a significant role in the coating process.…”

Section: Introductionmentioning

confidence: 99%

Influence of pH on formation and stability of phosphatidylcholine/phosphatidylserine coatings in fused-silica capillaries

2005

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The effect of pH on the formation and stability of phospholipid coatings in fused-silica capillaries in electrophoresis was investigated. A liposome solution consisting of 3 mM of 80:20 mol% phosphatidylcholine/phosphatidylserine (PC/PS) in N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) (HEPES) buffer was used as coating material. The coating was prepared by a method described earlier and five steroids were used as neutral model analytes. First, the effect of pH of the coating solution on the formation and stability of phospholipid coatings was studied at pH 6.5-8.5. The pH of the background electrolyte (BGE) solution (HEPES) was either kept constant at pH 7.4 or made similar to the pH of the liposome coating solution. Results showed that attachment of the coating on the fused-silica wall mostly depends on the protonation of amines of the phospholipids and HEPES. The ability of the phospholipid coating to withstand changes in pH was then investigated by coating at pH 7.5 and separating steroids with acetic acid, 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), HEPES, or glycine BGE, adjusted to pH between 4.5 and 10.8. The results showed that with use of BGE solution at pH 10.8, the separation of steroids was not successful and the electroosmotic flow was high because of leakage of the phospholipid coating during preconditioning of the capillary with BGE solution. There was no phospholipid leakage with a BGE solution of pH 4.5, indicating that the protonated form of the functional groups of PS and HEPES participating in the attachment of the phospholipid coating to the capillary play an essential role in the success of the coating.

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“…Thereafter, upon increasing phospholipid concentration in the mixtures, the van der Waals attraction between the phospholipid layer on latex and free vesicles in the dispersion would drive deposition of a phospholipid bilayer onto the monolayer covered latex (Carmona-Ribeiro & Herrington, 1993). The lipid coveredlatexes are useful as hosts for receptors (Sicchierolli & Carmona-Ribeiro, 1995;Sicchierolli & Carmona-Ribeiro, 1996;Moura & Carmona-Ribeiro, 2006), reduced protein adsorption on the polymer (Sicchierolli & Carmona-Ribeiro, 1996) and chromatography (Hautala et al, 2003;Haratake et al, 2007;Gulcev & Lucy, 2008). Although DODAC or DHP electrostatically adsorbed to oppositely charged polystyrene microspheres, forming homodisperse bilayer -covered latices, this took place only over a certain range of low lipid concentration.…”

Section: Bilayer Vesicle Particlesmentioning

confidence: 99%

Lipid-based Biomimetics in Drug and Vaccine Delivery

Carmona-Ribeiro¹

2010

Biomimetics Learning From Nature

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Simple coating of capillaries with anionic liposomes in capillary electrophoresis

Cited by 71 publications

References 41 publications

Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

Influence of pH on formation and stability of phosphatidylcholine/phosphatidylserine coatings in fused-silica capillaries

Lipid-based Biomimetics in Drug and Vaccine Delivery

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