Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

Lindén, Maria V.; Holopainen, Juha M.; Laukkanen, Antti; Riekkola, Marja‐Liisa; Wiedmer, Susanne Κ.

doi:10.1002/elps.200600002

Cited by 20 publications

(11 citation statements)

References 40 publications

(61 reference statements)

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“…As seen from Figure 4 the coating based on lipid extraction is well comparable with the coating based on membrane suspensions in terms of surface charge and stability. The results are also in good agreement with the red blood cells (RBC) [15] studies by Lindén et al which according to our knowledge are the only OT-CEC studies where a lipid extract of a cell membrane has been used for capillary coating. Repetition of the entire procedure with a new cell cultivation gave a coating with faster EOF mobility (2.8·10-8 m 2 s-1V-1) which indicates that the lipid concentration in the coating solution might have been too low.…”

Section: Membrane Suspension Vs Membrane Lipid Extractionsupporting

confidence: 90%

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Study of cell membrane based coatings in capillary electrochromatography

et al. 2010

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Neuronal cell line based membrane models were studied as capillary coatings by capillary electrochromatography. The membrane models were based either on membrane suspensions or on membrane lipid extractions. The stability of the coatings at different pH values and buffer compositions was studied. The results showed that the cell membrane suspension based coatings were stable over pH range of 6.5-10.8. The use of Hepes instead of TE buffer did not improve the coating performance.

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Section: Membrane Suspension Vs Membrane Lipid Extractionsupporting

confidence: 90%

“…However, nearly all of the studies have concentrated on well-controlled mixtures of two or three different lipids, often synthetical phospholipids. One of the few exceptions has been a study on human red blood cell (RBC) ghost lipids as a capillary coating [15].…”

Section: Introductionmentioning

confidence: 99%

Study of cell membrane based coatings in capillary electrochromatography

et al. 2010

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“…This may lead to enhanced coating stability and less desorption during the analysis and, as a result, minimize interferences in subsequent MS analysis. Linden et al previously studied capillary coatings composed of mixtures of 1,2-dipalmitoyl-sn-phosphatidylcholine (DPPC), SM, and cholesterol [30]. A moderately fast EOF (3 Â 10 À4 cm 2 V À1 s…”

Section: Introductionmentioning

confidence: 99%

Sphingomyelins as semi‐permanent capillary coatings for protein separations in CE and off‐line analysis with MALDI‐MS

Jurčić

Yeung

2009

Electrophoresis

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Phosphatidylcholine (PC) is one of the major phospholipids that make up the biological cell membrane. It was previously reported to form capillary inner wall coatings for CE. The zwitterionic head group of PC produced a neutral net-charged bilayer, which was found effective in preventing wall adsorption of both cationic and anionic proteins [J. M. Cunliffe et al. Anal. Chem. 2002, 74, 776-783]. Another major membrane phospholipid that possesses a zwitterionic head group is sphingomyelin (SM). In this work, the novel characterization of SM on its effectiveness in capillary coating formation for CE separations of proteins and peptides was presented. Similar properties were observed between PC and SM, including their effects on the EOF, peak efficiencies, and migration time reproducibilities. SM appeared to be more readily soluble in aqueous solutions, and it was found equally effective as PC in facilitating protein separation. The main difference observed was their performances in delivering a peptide mixture for off-line analysis with MALDI-MS. Superior sample recovery was evident from the capillary coated with SM compared with that with PC. The number of peptides identified from a 1 ng/microL myglobin tryptic digest sample increased from 5 to 16 (42 and 69%, respectively in protein sequence coverage).

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“…For example for progesterone (the most hydrophobic compound) the retention factor decreased from 1.47 to 0.38 when cholesterol was included in the liposomes. Cholesterol is generally considered to increase the rigidity of lipid membranes, affecting (decreasing) the interactions between steroids and the phospholipid stationary phase in open tubular CEC .…”

Section: Resultsmentioning

confidence: 99%

Study on capillaries covalently bound with phospholipid vesicles for open‐tubular CEC and application to on‐line open‐tubular CEC‐MS

2013

Self Cite

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Phospholipid vesicles were covalently attached to iminoaldehyde-coated fused silica capillaries and applied to the separation of model steroids by open-tubular CEC (OT-CEC). The effects of reducing the formed Schiff's base with sodium borohydride and of the liposome composition on the stability of the coating were investigated. In addition, the studies were focused on the optimization of running conditions (pH values and composition of BGE solution) when CEC, using capillaries covalently bound with liposome dispersions, was coupled to MS. The effect of cholesterol in the liposome dispersion on the binding of model analytes was studied, using liposome dispersions comprising 80/20 mol% zwitterionic 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and the negatively charged phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and 40/40/20 mol% POPC/POPS/cholesterol. Cholesterol in liposomes (greatly) enhanced the stability of the capillaries by making the coatings more rigid, resulting in lower retention factors for all the studied model steroids. Although most of the studies were carried out by open tubular CEC-UV Vis, the applicability of the capillaries to on-line CEC-MS was demonstrated as well. On-line CEC-MS studies on model steroids proved the suitability of coated capillaries for analyte-lipid membrane interaction studies, and especially for such analytes that are difficult to detect by conventional on-line UV Vis.

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Cholesterol‐rich membrane coatings for interaction studies in capillary electrophoresis: Application to red blood cell lipid extracts

Cited by 20 publications

References 40 publications

Study of cell membrane based coatings in capillary electrochromatography

Study of cell membrane based coatings in capillary electrochromatography

Sphingomyelins as semi‐permanent capillary coatings for protein separations in CE and off‐line analysis with MALDI‐MS

Study on capillaries covalently bound with phospholipid vesicles for open‐tubular CEC and application to on‐line open‐tubular CEC‐MS

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