Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC–MS/MS

Optimisation of the sensitivity of an immunoassay analysis for tobramycin in serum

“…This method was validated for accuracy and precision [12,13], having within-run, between-run and overall CVs lower than 20% for the LLOQ and lower than 15% for other QC levels (Tables 1 and 2). The method has a range of 0.03 to 0.6 mg/L, which was comparable to LC-MS/MS methods used in earlier studies [5,6]. Since kanamycin and amikacin cross-react with the tobramycin assay, this assay cannot be used to accurately quantitate tobramycin serum (or plasma) levels in patients receiving any of these drugs in combination with tobramycin.…”

“…Therefore, analytical methods using liquid chromatography-mass spectrometry (LC-MS) and other more complex LC methods have been developed. These methods often need derivatisation of the samples before tobramycin can be detected [5][6][7][8][9], this is time-consuming and expensive. Furthermore, the analytical robustness of these methods at lower limit of quantification (LLOQ) is limited.…”

Influence of Borneol on In Vitro Corneal Permeability and on In Vivo and In Vitro Corneal Toxicity

“…23 -25 Highperformance liquid chromatography-mass spectrometry (HPLC-MS) was used to measure ribavirin and tobramycin levels (Finnigan TSQ ® Quantum Discovery Max; Thermo Fisher Scientific) (HP 1100 series; Agilent Technologies), using Luna C 18 (Thermo Fisher Scientific) or XTerra RP C 18 (Waters Corp., Milford, MA, USA) columns, respectively. 26,27 Table 1 summarizes the test methods and criteria.…”

Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction

“…However, the sensitivity, and time consumption have greatly affected application of these methods. Attema-de Jonge et al [24] developed and validated a novel method for the quantification of low concentrations of tobramycin in human plasma with MS/MS detector, while this detector is not readily available in all laboratories. A direct HPLC analysis of tobramycin using UV or fluorescent detection is not straight- forward easily because there is no significant chromophore or fluorophore in structure of tobramycin.…”