Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction

He, Shun; Chen, Qiyou; Sun, Yanyan; Zhu, Yuncong; Luo, Laixin; Li, Jianqiang; Cao, Yongsong

doi:10.1016/j.jchromb.2011.02.042

Cited by 19 publications

(6 citation statements)

References 36 publications

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“…Tobramycin is often used to combat difficult infection such as osteomyelitis [35] and other serious gram negative bacillary infections [36]. This aminoglycoside chemically is similar to a sugar complex but presents challenges for HPLC analysis as it lacks a chromophore [35].…”

Section: Metronidazolementioning

confidence: 99%

“…This aminoglycoside chemically is similar to a sugar complex but presents challenges for HPLC analysis as it lacks a chromophore [35]. Because of this, pre-column derivatization techniques are often employed to make this drug visible to an associated UV detector [36,37]. The method utilized in this paper is similar to presented by Lai of Varian Chromatography Systems [37].…”

Section: Metronidazolementioning

confidence: 99%

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Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Corbett¹

2013

Mod Chem Appl

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Capability to quantify drug release is vital in many areas of pharmaceutical research. Accurate, rapid and repeatable detection of pharmaceutical agents allows for comparisons between drug delivery constructs prior to in vivo studies as well as identification of therapeutic levels in samples both in vitro and in vivo. Perhaps the foremost scientific method for monitoring of therapeutics is High Performance Liquid Chromatography. This process allows for clear and simple quantification of therapeutic presence in a sample, with an inherent or induced chromophore, through the detection of bound specimen to the utilized column. Multiple drugs can be used through this process and exemplary methods are presented for the pharmaceutics; cefuroxime, clindamycin, dexamethasone, dicloxacillin, doxycycline, metronidazole, oxymetazoline, paclitaxel, tobramycin, and vancomycin. Each of these drugs is analyzed using Reversed-Phase High Performance Liquid Chromatography utilizing a hydrophobic column. Independent, repeatable methods are developed for each drug. Where necessary a pre-column derivatization is used to allow for visualization of otherwise undetectable tobramycin. In the case of each drug, a standard curve is presented to show the linearity of response for the proposed method within a range of absorbance. This work shows the ability to analyze multiple drugs with a single simple, quick, cost effective system and detection methods are evaluated for each drug with a R 2 value of greater than 0.99.

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Section: Metronidazolementioning

confidence: 99%

Section: Metronidazolementioning

confidence: 99%

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Corbett¹

2013

Mod Chem Appl

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“…A literature survey revealed some reversed phase liquid chromatographic methods for the quantitation of tobramycin employing mass detector (Keevil et al, 2003;Guo et al, 2006), ELSD (Megoulas et al, 2005;Pfeifer et al, 2015) and PED (Manyanga et al, 2013). The literature showed also reversed phase HPLC methods with pre column derivatization with 1 Naphthyl isothiocyanate (Feng et al, 2002), Fluorescein isothiocyanate (Mashat et al, 2008), 2,4,6-Trinitrobenzenesulphonic acid (Dash andSuryanarayanan, 1991a;Dash and Suryanarayanan, 1991b), 4-Chloro-3,5-dinitrobenzotrifluoride (He et al, 2011), 9-Fluorenylmethyl chloroformate (Zhang and Peng, 2012) and o-Phthalaldehyde pre column derivatization (Caturla et al, 1992) and post column derivatization (He and Yang, 2010). This work describes the analysis of tobramycin in six formulations maintained in stability studies and quantitated using USP method (The United States Pharmacopeia, 2015).…”

Section: Introductionmentioning

confidence: 99%

Determination of the Chemical Stability of Various Formulations of Tobramycin Eye-Drops by HPLC Method and Data Analysis by R-GUI Stability Software

Rosasco¹,

Segall

2015

J App Pharm Sci

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Tobramycin, is a water soluble aminoglycoside antibiotic produced by the fungus Streptomyces tenebrarius and used in a variety of pharmaceutical applications including ophthalmic solutions, suspensions and ointments; inhalation solutions and intravenous administration. There are commercially available eye drops formulations in the Argentinian market that have different conservation conditions. We formulated six eye drops solutions, studied their stability at 2-8°C, 25°C, and 40°C, 75% RH and quantified using USP method. Only half of the formulations studied were found to be stable for two years at ambient temperature which is their expected expiry date and only one for three years in the same condition. One system was unstable in the three conservation conditions studied, including appearance and pH.

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“…Some analytical methods have already been reported for the determination of AG residues, such as immunoassays , CE , GC‐MS , and HPLC coupled with evaporative light scattering detection , UV detection , fluorescence detection , or MS detection . Among these analysis methods, HPLC is the main analytical method for determining AGs.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid‐based microwave‐assisted surfactant‐improved dispersive liquid–liquid microextraction and derivatization of aminoglycosides in milk samples

Liu

Zhao

et al. 2013

J of Separation Science

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A green and simple method, ionic liquid-based microwave-assisted surfactant-improved dispersive liquid-liquid microextraction and derivatization was developed for the determination of aminoglycosides in milk samples. Nonionic surfactant Triton X-100 and ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate were used as the disperser and extraction solvent, respectively. Extraction, preconcentration, and derivatization of aminoglycosides were carried out in a single step. Several experimental parameters, including type and volume of extraction solvent, type and concentration of surfactant, microwave power and irradiation time, concentration of derivatization reagent, and pH value and volume of buffer were investigated and optimized. Under the optimum experimental conditions, the linearities for determining the analytes were in the range 0.4-10.0 ng/mL for tobramycin, 1.0-25.0 ng/mL for neomycin, and 2.0-50.0 ng/mL for gentamicin, with the correlation coefficients ranging from 0.9991 to 0.9998. The LODs for the analytes were between 0.11 and 0.50 ng/mL. The present method was applied to the analysis of different milk samples, and the recoveries of aminoglycosides obtained were in the range 96.4-105.4% with the RSDs lower than 5.5%. The results showed that the present method was a rapid, convenient, and environmentally friendly method for the determination of aminoglycosides in milk samples.

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Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction

Cited by 19 publications

References 36 publications

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Determination of the Chemical Stability of Various Formulations of Tobramycin Eye-Drops by HPLC Method and Data Analysis by R-GUI Stability Software

Ionic liquid‐based microwave‐assisted surfactant‐improved dispersive liquid–liquid microextraction and derivatization of aminoglycosides in milk samples

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