Simple and robust high-performance liquid chromatographic method for the determination of ranitidine in microvolumes of human serum

A single LC–tandem mass spectrometry method for the simultaneous determination of four H2 antagonists in human plasma

“…A number of analytical methods have been reported for the determination of individual H 2 antagonists in human plasma since 1990 years, cimetidine [1][2][3][4][5][6][7][8], ranitidine [9][10][11][12][13][14][15][16][17], famotidine [18][19][20][21][22][23][24][25], and lafutidine [26][27][28][29]. Apart from some uncommonly used methods such as free capillary zone electrophoresis [4], micellar electrokinetic capillary chromatography [7], HPTLC [13], most reports utilized HPLC-UV method [1][2][3][4][5][6][7][9][10][11][12][13][14][15][16][18][19]…”

“…Apart from some uncommonly used methods such as free capillary zone electrophoresis [4], micellar electrokinetic capillary chromatography [7], HPTLC [13], most reports utilized HPLC-UV method [1][2][3][4][5][6][7][9][10][11][12][13][14][15][16][18][19][20][21][22][23]26,27]. Although HPLC method is mature and relatively economical, limitations such as sensitivity, selectivity and throughput were often encountered during the method development.…”

Liquid chromatographic determination including simultaneous “on-cartridge” separation of ranitidine cisapride drug combinations from paediatric plasma samples using an automated solid-phase extraction procedure

“…The method employed [3] was a modification of that described by López-Calull et al [9] using AH20480 as the internal standard. The analytical column was a Waters Bondapak C18 (300 mm × 3.9 mm, 10 m) fitted with a Waters Bondapak C18 (3.9 mm × 20 mm, 10 m) guard column.…”

“…The analysis of ranitidine in human plasma is frequently performed using HPLC [3][4][5][6][7][8][9][10][11][12][13][14]. Although HPLC methods for the determination of plasma ranitidine concentrations vary widely, isocratic reverse-phase HPLC using a -Bondpack C18 packed analytical column appears to be the most common mode selected [3,4,9,10,12,13].…”

“…Although HPLC methods for the determination of plasma ranitidine concentrations vary widely, isocratic reverse-phase HPLC using a -Bondpack C18 packed analytical column appears to be the most common mode selected [3,4,9,10,12,13]. Chromatographic separation of ranitidine from plasma constituents has been achieved using a wide variety of mobile phase conditions, however, in more recent years a phosphate-buffered acetonitrile mobile phase has frequently been chosen [3,[7][8][9]12,14]. Despite such a wide variety of HPLC methods it is only recently that a method suitable for the analysis a paediatric plasma samples has been reported [15].…”