Simple and rapid method for the simultaneous determination of the non-nucleoside reverse transcriptase inhibitors efavirenz and nevirapine in human plasma using liquid chromatography

A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

“…For sample pretreatment, 100 μL plasma was mixed with 200 μL acetonitrile (with 1 μg/mL diazepam) to precipitate protein, and the mixture was centrifuged and then the supernatant was directly injected into the HPLC system. In contrast to solid-phase extraction, liquid-liquid extraction (23,24,(26)(27)(28)(32)(33)(34)(35)38,(44)(45)(46), this method has advantages of simplicity, rapidity, economical and low requirement for plasma volume, suitable for pediatric patients. As shown in Figure 1, endogenous substances have no interference on the peak of efavirenz and diazepam, and the column pressure have no obvious rise after the determination of over 400 plasma samples, which indicated that plasma proteins could be precipitated completely in this method.…”

“…On the contrary, reverse phase highperformance liquid chromatographic with ultraviolet detector (RP-HPLC-UV) method is feasible due to its easy accessibility and low cost. Also, many HPLC-UV methods for determination of plasma efavirenz (23,(26)(27)(28)(29)(30)(31) or simultaneous determination with other drugs such as anti-tuberculosis (anti-TB) agents or other antiretroviral agents (24,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) have been reported. In most of these methods, the volumes of plasma used range from 200 to 900 μL (23,(26)(27)(28)33,34,(44)(45)(46), which were inapplicable for children due to the scarcity of sample.…”

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

“…4,6,13,23 In 2006 Ramachandran et al 17 describes a bioanalytical method by HPLC with UV detection and application in 3 volunteers with 10 collected blood samples in different times after the administration of Efavirenz and did not show application of high-throughput samples.…”

“…This is because of its long half-life, which means effective concentrations are maintained for long periods. 3 Several methods are described in literature for the quantification of efavirenz in biological fluids by high performance liquid chromatography with ultraviolet detection (HPLC-UV), [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] HPLC with fluorescence detector, 23 liquid chromatography with mass spectrometry detection (LC-MS), 24 liquid chromatography with mass spectrometry in tandem detection(LC-MS/MS) [25][26][27][28][29] and matrix assisted laser desorption/ionization with time-of-flight in tandem detection (MALDI-TOF/ TOF). 30 When sample throughput is an important issue, such as in pharmacokinetic applications, the development of rugged methods with short analysis times becomes an important consideration.…”

Desenvolvimento de método analítico para quantificação do efavirenz por espectrofotometria no UV-Vis

“…2 Na literatura científica internacional, diversos métodos analí-ticos para a quantificação do EFZ são preconizados utilizando-se cromatografia líquida de alta eficiência (CLAE) do fármaco isolado e na forma farmacêutica cápsula, 3 assim como em plasma na forma isolada e associado a outros antirretrovirais. [4][5][6][7][8][9][10][11][12][13][14] Contudo, nenhum método analítico por espectrofotometria de absorção no ultravioleta visível (UV-vis) foi relatado.…”