Simple and rapid extraction method of total egg lipids for determining organochlorine pesticides in the egg

Furusawa, Naoto; Ozaki, Asako; Nakamura, Miyuki; Morita, Yukari; Okazaki, Kanako

doi:10.1016/s0021-9673(98)00873-5

Cited by 19 publications

(16 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Approximately, 60% of the weight of egg yolk powder is of lipids [15], and its emulsifying properties cause difficulties in SGP extraction. We investigated several organic solvents commonly used in the food industry for lipid extraction including acetone [16], diethyl ether [17], hexanes [18], chloroform/methanol mixtures [19,20], and ethanol [21]. Egg yolk powder was stirred in each solvent for 30 min, filtered, and the insoluble material was dried in vacuo to determine the weight loss due to the removal of the lipids (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

Liu

Prudden

Bosman

et al. 2017

Carbohydrate Research

View full text Add to dashboard Cite

Sialylglycopeptide (SGP) is a complex bi-antennary N-glycan bearing a short peptide fragment that can be isolated from the yolk of hen eggs. This natural product has gained popularity as a starting material for the semi-synthesis of N-glycans. We have found that current isolation methods provide a glycopeptide contaminated with several related structures, one being a glycopeptide having a hexose directly attached to peptide backbone, most like through the hydroxyl containing side chain of the threonine moiety. Furthermore, current methods employ fresh egg yolks that need to be lyophilized and involve several tedious purification steps. Herein, we report a convenient method for the isolation of gram quantities of homogeneous SGP from commercially available egg yolk powder using solid/liquid extraction and HILIC-HPLC purification.

show abstract

Section: Resultsmentioning

confidence: 99%

Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

Liu

Prudden

Bosman

et al. 2017

Carbohydrate Research

View full text Add to dashboard Cite

show abstract

“…Tissue samples (adipose, liver, and blood) were collected for analytical chemistry, along with one or two egg yolks from each of the females that oviposited. Tissues and yolks were screened for 30 OCP analytes by GC-MS. Lipid content (%) was determined gravimetrically for liver, and adipose, while GC-MS techniques were used for blood (Furusawa, Ozaki, Nakamura, Morita, & Okazaki, 1999).…”

mentioning

confidence: 99%

Achieving environmentally relevant organochlorine pesticide concentrations in eggs through maternal exposure in Alligator mississippiensis

Rauschenberger

Wiebe²,

Buckland³

et al. 2004

Marine Environmental Research

View full text Add to dashboard Cite

“…The quantitative analyses for DDTs in all samples were performed using an extraction procedure, as described previously (Furusawa et al., 1999; Furusawa, 2001). An accurately weighed amount (10 g) of sample was mixed with a suitable amount of anhydrous sodium sulphate.…”

Section: Methodsmentioning

confidence: 99%

“…After centrifugation at 1164 g for 5 min, the supernatant was poured into a separating funnel through a funnel packed with anhydrous sodium sulphate. The dried hexane layer was collected and evaporated to dryness, and the lipid content was obtained (Furusawa et al., 1999).…”

Section: Methodsmentioning

confidence: 99%

Transferring and Distributing Profiles of p,p′‐(DDT) in Egg‐forming Tissues and Eggs of Laying Hens Following a Single Oral Administration

Furusawa¹

2002

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

Laying hens were administered orally with a single dose of p,p'-(DDT) (1 mg/kg bodyweight). The concentrations (microg/g) of DDT or its metabolites, p,p'-(DDE) and p,p'-(DDD), in the main tissues involved in egg formation (blood, liver, ovary, and oviducts) and egg yolk, collected 1 day after DDT dosing, were determined by normal-phase high-performance liquid chromatography. The limits of detection were 0.04 microg/g for DDT, 0.07 microg/g for DDE and 0.06 microg/g for DDD. In extractable fats from the above tissues and egg yolk, DDT and DDE were transferred/distributed throughout the tissues and egg yolk. DDD was detected only in the liver. The findings indicate that DDT is metabolized instantaneously to DDE/ DDD in the hen's body and they are transferred rapidly into the egg-forming tissues and egg yolk. Among the four tissues and yolk fats examined, the DDT levels were high in the ovary, oviduct and egg yolk; the DDE levels were high in the liver, ovary and oviduct and lowest in the yolk (P < 0.01).

show abstract

Simple and rapid extraction method of total egg lipids for determining organochlorine pesticides in the egg

Cited by 19 publications

References 2 publications

Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

Achieving environmentally relevant organochlorine pesticide concentrations in eggs through maternal exposure in Alligator mississippiensis

Transferring and Distributing Profiles of p,p′‐(DDT) in Egg‐forming Tissues and Eggs of Laying Hens Following a Single Oral Administration

Contact Info

Product

Resources

About