Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine

Shimomura, Mifumi; Nomura, Yoko; Zhang, Wei; Sakino, Masato; Lee, Kyong-Hoon; Ikebukuro, Kazunori; Karube, Isao

doi:10.1016/s0003-2670(01)00809-1

Cited by 94 publications

(36 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the simplicity and cost-effectiveness for detecting the fungicide residues, immunoassays have recently gained increasing interest as an alternative method for large monitoring programs (Lequin 2005). The immunological method is an efficient screening technique for the monitoring of illegal and harmful chemicals in food and the environment, and these assays offer significant advantages, such as speed and high sample throughput (Shimomura et al 2001). Consequently, immunoassay formats such as enzyme-linked immunosorbent assay (ELISA), fluorescence polarisation (FPIA), and time-resolved fluorescence (TR-FIA) offer easy-to-use, rapid, and sensitive methods that have been successfully utilised for the detection of fungicides.…”

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Yang¹,

Zhu²,

Liu³

et al. 2016

Czech J. Food Sci.

View full text Add to dashboard Cite

Yang W., Zhu J., Liu M.Q., Dai X.J. (2016): Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges. Czech J. Food Sci., 34: 429-438.To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active group, (E)-2-(2-bromophenyl)-2-(methoxyimino) acetic acid methyl ester (OEBr) in kresoxim-methyl and trifloxystrobin. The immunogen and coating antigen were respectively prepared through conjugating the above-mentioned hapten with BSA and OVA by the mixed anhydride and activated ester methods, and polyclonal antibodies were produced by immunised rabbits. An enzyme-linked immunosorbent assay was developed for simultaneous detection of kresoxim-methyl and trifloxystrobin. In ELISA, the antiserum showed high affinity and sensitivity to kresoxim-methyl and trifloxystrobin, and their IC 50 value and detection limit (expressed as IC 10 ) were 14.7 and 0.0044 µg/ml, respectively, for kresoxim-methyl, and 22.9 and 0.014 µg/ml, respectively, for trifloxystrobin. The cross-reaction rate was below 0.1% for other strobilurin fungicides. Recovery study of ELISA from spiked samples of homogenised peeled oranges (final concentrations of 100, 10, and 1 µg/ml) resulted in recovery levels in the range of 82-104%.

show abstract

Section: Kresoxim-methyl Methyl(e)-methoxyimino[α-(o-tolyloxy)-o-tolmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Yang¹,

Zhu²,

Liu³

et al. 2016

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…Studies on environmental PCB determinations mainly focus on these congeners. [12][13][14][15][16] Given their high toxicities relative to other congeners, assays capable of quantifying one or all of these congeners need to be established essentially.…”

Section: -9mentioning

confidence: 99%

Detection of PCB77 by Indirect Competitive Enzyme-linked Immunosorbent Assay in Sea Sediment Samples

Chen¹,

Zhuang²,

Yang³

2013

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Tetrachlorobiphenyl (IUPAC PCB77) is one of seven indicative polychlorinated biphenyls (PCBs) in the surface sediments. The current study presents a novel polyclonal antibody for the determination of the PCB77 using indirect competitive enzyme-linked immunosorbent assay. Under optimum conditions, PCB77 was determined within the concentration range of 0.01-100 µg L -1, with a detection limit of 0.057 µg L -1. The assays were tested for their cross-reactivity profiles using 3 selected congeners and 4 Aroclor products. The assays were highly specific for coplanar PCB congeners, but less specific for Aroclor1248. The spiked recoveries from five sediment samples were 86%-114% for PCB77 from ELISA, which were satisfactory. The current study demonstrated that the developed antiserum and immunoassay procedure can be used to detect PCB77 in environmental samples. The results of the sediment analysis were confirmed by conventional GC/ ECD.

show abstract

“…The high toxicity of some PCB congeners represents a risk for public health as these compounds are still present in the environment, even though the production of PCBs has been banned in several countries many years ago. Different biosensor configurations have been designed to determine PCBs in the environment, and these include the DNA biosensor with chronopotentiometric detection [59], and various immunosensors with fluorescence [60], SPR [61], and electrochemical [62] detection principles.…”

Section: Pcbsmentioning

confidence: 99%

“…Thus, processes such as the production of some pesticides, the manufacture of PVC plastics, the chlorine bleaching of pulp and paper and waste incineration generate dioxins. They are considered carcinogenic and are a potential threat to human health [63], and more recently, they have been included in lists of potential EDCs [61]. Conventional dioxins analysis requires laborious multistep clean-up procedures that increase the cost of each analysis.…”

Section: Dioxinsmentioning

confidence: 99%

“…A significant number of immunoassays for dioxins have been developed in an effort to provide simplified and routine analysis [63][64][65]. The SPR biosensor developed by Shimomura et al [61] for the determination of PCB (mentioned above) was also employed in the determination of the dioxin 2,3,7,8-TCDD. Similarly, another biosensor for detection of dioxin-like chemicals (polyhalogenated dioxins, furans, and biphenyls) based on a recombinant mouse hepatoma cell line was characterized and optimized by Pasini et al [66].…”

Section: Dioxinsmentioning

confidence: 99%

See 1 more Smart Citation

Biosensors for environmental applications: Future development trends

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Pure and Applied Chemistry

197

View full text Add to dashboard Cite

Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. In this context, bioelectronics, nanotechnology, miniaturization, and especially biotechnology seem to be growing areas that will have a marked influence on the development of new biosensing strategies in the next future.

show abstract

Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine

Cited by 94 publications

References 24 publications

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Detection of PCB77 by Indirect Competitive Enzyme-linked Immunosorbent Assay in Sea Sediment Samples

Biosensors for environmental applications: Future development trends

Contact Info

Product

Resources

About