Simple and Rapid Detection for Rice stripe virus Using RT-PCR and Porous Ceramic Cubes

Abstract

A rapid and simple RT-PCR diagnosis method for detection of Rice stripe virus (RSV), one of major virus infecting rice, was developed using porous ceramic cubes in this study. The porous ceramic cube can rapidly absorb biological molecules such as small-sized proteins and nucleic acid fragments into its pores. We examined whether this ability of porous ceramic cubes could be applied for isolating viral nucleic acids or particles from the RSV-infected plant tissues. In this study, we found that the porous ceramic cube was capable of absorbing a detection level of viruses from the rice tissues infected with RSV and established RT-PCR-based RNA diagnosis method using porous ceramic cubes.

Incidence and Molecular Identification of Begomoviruses Infecting Tomato and Pepper in Myanmar

“…Samples were collected as dried leaf samples or using Biocube (Biocubesystem, Suwon, Korea) from leaf tissues according to the manufacturer's instructions. The Biocube, which is a porous ceramic cube with a pore size of about 160-340 nm, can rapidly absorb nucleic acid fragments into its pores and be added directly into a polymerase chain reaction (PCR) tube as a template without any solvent extraction process [39]. When samples could not be transported directly, due to quarantine, or when it was difficult to maintain freshness for a long time, virus-infected samples are collected using a Biocube folder.…”