Simple and rapid analytical method for detection of amino acids in blood using blood spot on filter paper, fast-GC/MS and isotope dilution technique

Kawana, Shuichi; Nakagawa, Katsuhiro; Hasegawa, Yuki; Yamaguchi, Seiji

doi:10.1016/j.jchromb.2010.09.015

Cited by 20 publications

(9 citation statements)

References 38 publications

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“…19 Elevated Cit, Met, Thr, Tyr, Lys, Arg, and Orn in the blood and urine of patients with NICCD has been reported previously. 10,20,21 In this study, the concentration of Cit, Arg, Orn, Met, and Tyr in the blood of patients with NICCD was higher than in controls and patients with BA. We used Cit (> 55 µmol/L) and Arg (> 30 µmol/L) to diagnose NICCD and obtained satisfy sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 41%

Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency Differentiated from Biliary Atresia

Tian

Zhang

et al. 2015

Eur J Pediatr Surg

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Purpose The aim of this article is to differentiate neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) from biliary atresia (BA) by total hexose. Methods A total of 11 patients with NICCD, 29 patients with BA, and 4,898 children as controls were involved in this study. The blood concentration of amino acids, carnitine, acylcarnitines, and total hexose were measured in dry blood spots (DBS) using tandem mass spectrometry (MS/MS). Results In the patients with NICCD, the blood concentration of the total hexose (15.3 ± 9.0 mmol/L vs. 7.3 ± 2.7 mmol/L; p < 0.001), citrulline (Cit) (197.9 ± 93.7 µmol/L vs. 17.5 ± 7.4 µmol/L; p < 0.001) were higher than those of patients with BA. Using total hexose (> 10 mmol/L), Cit (> 55 µmol/L) to diagnose NICCD, the sensitivity and specificity were 66.7 and 97.8% and 90.0 and 99.1%, respectively, and all of the areas under the receiver-operating characteristic curves were greater than 0.85. Conclusion Elevated total hexose in DBS measured by MS/MS associated with elevated amino acids, especially Cit can be used to diagnose NICCD and differentiate it from BA.

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Section: Discussionmentioning

confidence: 41%

Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency Differentiated from Biliary Atresia

Tian

Zhang

et al. 2015

Eur J Pediatr Surg

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“…In determining the essential amino acid Phe, optical methods were used, respectively: colorimetry [22,23]. UV and IR spectrophotometry [24], fluorescence [25][26][27][28][29], enhanced fluorescence [30], chemiluminescence [31][32][33]; separation methods, such as: gas chromatography [34], capillary electrophoresis, HPLC-high performance liquid chromatography [22,[35][36][37][38][39][40][41][42][43][44]; spectroscopic methods: Raman spectroscopy [45,46], laser-assisted spectroscopy [47], UV-Vis spectroscopy and chemometry [48]; UV−Vis and surface-enhanced Raman scattering [49], nuclear magnetic resonance [50] and circular dichroism spectroscopy [51]; mass spectrometry [22,34,41,52], fluorimetry [53].…”

Section: Instrumental Methodsmentioning

confidence: 99%

A Review on Electrochemical Sensors and Biosensors Used in Phenylalanine Electroanalysis

Dinu

Apetrei

2020

Sensors

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Phenylalanine is an amino acid found in breast milk and in many foods, being an essential nutrient. This amino acid is very important for the human body because it is transformed into tyrosine and, subsequently, into catecholamine neurotransmitters. However, there are individuals who were born with a genetic disorder called phenylketonuria. The accumulation of phenylalanine and of some metabolites in the body is dangerous and may cause convulsions, brain damage and mental retardation. Determining the concentration of phenylalanine in different biologic fluids is very important because it can provide information about the health status of the individuals envisaged. Since such determinations may be made by using electrochemical sensors and biosensors, numerous researchers have developed such sensors for phenylalanine detection and different sensitive materials were used in order to improve the selectivity, sensitivity and detection limit. The present review aims at presenting the design and performance of some electrochemical bio (sensors) traditionally used for phenylalanine detection as reported in a series of relevant scientific papers published in the last decade.

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“…HPLC baseline separation of leucine and isoleucine + allo isoleucine is observed. Use of a derivative with high sensitivity under electron impact conditions has recently been described for fast GC/MS analysis of these amino acids in bloodspots [11]. Another positive attribute of the DMABS derivatizing reagent for bloodspot analysis is its lack of reactivity with amide groups.…”

Section: Preparation and Testing Of Isotope-labelled Derivatizing Reamentioning

confidence: 99%

Free amino acid quantification by LC–MS/MS using derivatization generated isotope-labelled standards

Johnson

2011

Journal of Chromatography B

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Simple and rapid analytical method for detection of amino acids in blood using blood spot on filter paper, fast-GC/MS and isotope dilution technique

Cited by 20 publications

References 38 publications

Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency Differentiated from Biliary Atresia

Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency Differentiated from Biliary Atresia

A Review on Electrochemical Sensors and Biosensors Used in Phenylalanine Electroanalysis

Free amino acid quantification by LC–MS/MS using derivatization generated isotope-labelled standards

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