Simple and on‐site DNA purification for LAMP reaction applicable to non‐adult tephritid fruit fly (Diptera: Tephiritidae)

Kitano, Daisuque; Takakura, Koh‐Ichi

doi:10.1111/jen.12820

Cited by 14 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quick DNA extraction method was first applied to Plasmodium falciparum in LAMP assay using a heat‐based (99℃ for 10 min) treatment to prepare DNA for LAMP rapidly, and compared with the column or organic solvent‐based DNA extraction method (Poon et al., 2006). In 2020, quick and simple DNA preparation was applied to fruit flies for the first time with the improved ‘toothpick method’ adapted from Fukuta et al (2005) (Kitano & Takakura, 2020). The ‘toothpick method’ was applied and improved in our research.…”

Section: Discussionmentioning

confidence: 99%

“…To prepare DNA of target species quickly, sterile toothpicks were used for quick extraction method modified from Kitano and Takakura's method (2020). First, living larvae and pupae were washed with sterilized distilled water, and the insects were removed onto a filter paper to dry.…”

Section: Methodsmentioning

confidence: 99%

“…Regulated quarantine insect species Thrips palmi Karny (Thysanoptera: Thripidae) , Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and several regulated fruit fly species in the genera Bactrocera and Zeugodacus as target pests for LAMP assay were identified successfully at POEs (Blaser et al, 2018). Some LAMP primers had been designed with success for Dacus ciliatus Loew (Diptera: Tephritidae) (Sabahi et al, 2018), Ze ugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) (Zhong et al., 2019), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) (Blacket et al., 2020) and Zeugodacus scutellatus Hendel (Diptera: Tephritidae) (Kitano & Takakura, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…For on-site detection with the naked eye, colorimetric LAMP assay has also been applied by adding many kinds of dyes before the reaction to avoid false positives of aerosol contamination caused by lid opening (Jia et al, 2018) for LAMP assay were identified successfully at POEs (Blaser et al, 2018). Some LAMP primers had been designed with success for Dacus ciliatus Loew (Diptera: Tephritidae) (Sabahi et al, 2018), Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) (Zhong et al, 2019), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) (Blacket et al, 2020) and Zeugodacus scutellatus Hendel (Diptera: Tephritidae) (Kitano & Takakura, 2020).…”

mentioning

confidence: 99%

See 3 more Smart Citations

A rapid LAMP‐based colorimetric assay with quick DNA extraction for on‐site identification of Drosophila suzukii Matsumura

Wang

Liu

Zhang

et al. 2021

J Applied Entomology

View full text Add to dashboard Cite

The spotted wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), distributes in most soft‐skinned fruit areas in China, is an economically important pest of fresh cherries during Sino‐European trade and is considered a quarantine pest in A2 list by European and Mediterranean Plant Protection Organization. Loop‐mediated isothermal amplification (LAMP) is an accurate, quick and convenient molecular identification method, applied to distinguish D. suzukii from other fruit flies. This method can be used for inspection in the field, and at the points of entry (POEs), because its results can be detected with the naked eye due to colour changes. In our study, first, we reported a simple and fast LAMP colorimetric detection method for molecular identification of D. suzukii. We designed primer sets for D. suzukii based on mitochondrial cytochrome oxidase I (COI) sequences. The specificity and sensitivity of the primers were tested by using target and non‐target fruit flies in the family Drosophilidae and Tephritidae, usually intercepted during the Sino‐European cherry trade. Second, for detection in the field and at POEs, the adoption of a quick DNA extraction method could save the total time of LAMP identification to about 90 min. Taken together, this accurate, quick and convenient LAMP‐based colorimetric identification assay combined with a quick DNA extraction method could visually detect clearly with just one portable heating device, which will be useful for rapid on‐site identification and inspection for D. suzukii by the quarantine department.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A rapid LAMP‐based colorimetric assay with quick DNA extraction for on‐site identification of Drosophila suzukii Matsumura

Wang

Liu

Zhang

et al. 2021

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…These were commercialized by Optigene ( http://www.optigene.co.uk/plant-health-pests-diagnostics ) and are used for species identification at two ports of entry in Switzerland ( von Felten, 2017 ). For a similar purpose, a LAMP kit was developed for rapid identification of Bactrocera flies ( Bactrocera dorsalis complex, Bactrocera cucurbitae / Bactrocera latifrons and Bactrocera correcta / Bactrocera zonata ) ( Blaser et al., 2018 ), while recently a LAMP identification method was reported for B. tryoni ( Blacket et al., 2020 ), Dacus ciliatus ( Sabahi et al., 2018 ) and Zeugodacus scutellatus ( Kitano & Takakura, 2020 ). In 2009, Huang et al.…”

Section: Introductionmentioning

confidence: 99%

A loop-mediated isothermal amplification (LAMP) assay for rapid identification of Ceratitis capitata and related species

Dermauw¹,

Moerkerke²,

Ebrahimi³

et al. 2022

Current Research in Insect Science

View full text Add to dashboard Cite

New and rapid visual detection assay for Trogoderma granarium everts based on recombinase polymerase amplification and CRISPR/Cas12a

Zeng,

Zheng,

Stejskal

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDKhapra beetle (Trogoderma granarium Everts), one of the most important quarantine pests globally, is capable of causing severe infestation and huge economic loss to stored grain, and its interception rate has increased in major global trade countries over the past few years. However, difficulties remain in distinguishing this species with similar ones. In order to assist border ports and warehouses in khapra beetle's effective rapid identification as well as pest control at the early stages of monitoring or interception, we herein developed a new and rapid visual detection assay for T. granarium based on recombinase polymerase amplification (RPA) and the CRISPR/Cas12a system.RESULTSWe designed and selected the first khapra beetle‐specific RPA primers and crRNA, and optimized the visualization reaction system (Cas12a/CrRNA = 100 nM/500 nM). With only a 37 °C‐heat‐source and a blue light torch, RPA and CRISPR/CAS12a‐based visualization assays can be completed within 40 min to differentiate between khapra beetle and nine similar Dermestidae species. After DNA extraction using a kit (4–5 h) or a simple method (5 min), the specific amplicons were obtained after a 15 min RPA reaction at 37 °C, followed by a 15 min color reaction under 37 °C in dark conditions using a CRISPR/CAS12a system and a fluorescent probe (5′‐FAM/3′‐BHQ1 labeled). This method is ingenious to low levels of DNA (10−1 ng μL−1) and meets the sensitivity requirements for detecting a single khapra beetle's egg (≈0.7 mm).CONCLUSIONOur specificity and sensitivity analysis inferred that the present visualization system is effective to quickly and uniquely detect khapra beetle at room temperature (37 °C), thereby preventing this species before they spread widely. Our study is suitable for being pushed forward in storage pest management, and provides value as a reference for monitoring and identification of other pests. © 2023 Society of Chemical Industry.

show abstract

Simple and on‐site DNA purification for LAMP reaction applicable to non‐adult tephritid fruit fly (Diptera: Tephiritidae)

Cited by 14 publications

References 28 publications

A rapid LAMP‐based colorimetric assay with quick DNA extraction for on‐site identification of Drosophila suzukii Matsumura

A rapid LAMP‐based colorimetric assay with quick DNA extraction for on‐site identification of Drosophila suzukii Matsumura

A loop-mediated isothermal amplification (LAMP) assay for rapid identification of Ceratitis capitata and related species

New and rapid visual detection assay for Trogoderma granarium everts based on recombinase polymerase amplification and CRISPR/Cas12a

Contact Info

Product

Resources

About