A loop-mediated isothermal amplification (LAMP) assay for rapid identification of Ceratitis capitata and related species

Dermauw, Wannes; Moerkerke, Yannick Van; Ebrahimi, Negin; Casteels, Hans; Bonte, Jochem; Witters, Johan

doi:10.1016/j.cris.2022.100029

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…High discrimination ability among meats from blue swimming crab, crucifix crab, and three spotted swimming crab was confirmed, although the sequence of the COI gene between these crabs showed a 82–85% similarity. One of the benefits of using LAMP for food authentication is that less effort of optimization for specificity is required since the reaction is mediated by 2–3 pairs of primers recognizing up to eight certain regions on the DNA target [ 26 , 27 ]. This is different from PCR, which uses a pair of primers for target amplification, making PCR tend to show a low specificity if close species are used [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Three Swimming Crab Species

Benjakul

Saetang

2022

Foods

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Blue swimming crab meat is easily adulterated by other crab meats with a lower price. A potential authentication method is required to prevent mislabeling. LAMP assays were established to identify the meat of blue swimming crab, crucifix crab, and three spotted swimming crab. The primers were designed using PrimerExplorer V5. The specificity of the LAMP assay was tested compared to the PCR method. The sensitivity was conducted at the DNA concentrations of 0.4–50 ng/reaction. The results demonstrated that both LAMP and PCR could discriminate all species of crabs. LAMP showed a superior sensitivity to PCR in the three spotted swimming crab, while a similar result between LAMP and PCR was obtained in blue swimming crab. No changes in the detection efficacy were attained when boiled and steamed crab meats were applied. Therefore, the LAMP assay developed could potentially be applicable to detect the adulteration or mislabeling of raw or cooked crab meat in markets.

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Section: Discussionmentioning

confidence: 99%

Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Three Swimming Crab Species

Benjakul

Saetang

2022

Foods

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“…These methods are increasingly used to identify pests and invasive species. LAMP, for example, is being used to identify quarantined species on-site (von Felten 2017, in Switzerland), especially fruit flies (Blaser et al 2018;Sabahi et al 2018;Blacket et al 2020;Kitano and Takakura 2020;Dermauw et al 2022), using a portable device. LAMP is also helpful for field identification, especially when specimens cannot be reliably identified using traditional methods (e.g., a stage or sex is not morphologically useful for accurate identification): Agarwal et al (2020; for the fall armyworm, Spodoptera frugiperda (J.E.…”

Section: Molecular Tools For Rapid Identificationmentioning

confidence: 99%

Taxonomy and Biological Control: New Challenges in an Old Relationship

et al. 2023

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Biological control and taxonomy are continuously developing fields with remarkable impacts on society. At least 80 years of literature have documented this relationship, which remains essentially the same in its mutualistic nature, as well as in its major challenges. From the perspective of Brazilian taxonomists, we discuss the impacts of important scientific and social developments that directly affect research in these areas, posing new challenges for this lasting relationship. The increasing restrictions and concerns regarding the international transit of organisms require improvements in research related to risk assessment for exotic biological control agents and also stimulate prospecting within the native biota. In our view, this is a positive situation that can foster a closer relationship between taxonomists and applied entomologists, as well as local surveys and taxonomic studies that are necessary before new programs and agents can be implemented. We discuss the essential role of molecular biology in this context, as an iconic example of the synergy between applied sciences and natural history. As our society comes to need safer and more sustainable solutions for food security and the biodiversity crisis, scientific progress will build upon this integration, where biological control and taxonomy play an essential role.

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“…LAMP technology has been widely used for species identification of pests, and the COI gene is commonly used as the target. The technology has been successfully applied to identify biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) (Hsieh 2012), regulated fruit flies of the genera Bactrocera (Diptera: Tephritidae) (Blaser 2018), Zeugodacus (Diptera: Tephritidae) (Zhong 2019), and Ceratitis (Diptera: Tephritidae) (Dermauw 2022). Compared with DNA barcoding, LAMP showed high efficiency and rapidity.…”

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confidence: 99%

LAMP Assay as a Rapid Identification Technique of Chinese Citrus Fly and Japanese Orange Fly (Diptera: Tephritidae)

Yang

Zeng

et al. 2023

Journal of Economic Entomology

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Bactrocera tsuneonis and Bactrocera minax are the most destructive pests that damage citrus in China. These key pests hinder the citrus trade, cause significant financial losses, drastically lower citrus production and quality, and decrease farmer enthusiasm for citrus planting. Bactrocera minax and B. tsuneonis are very similar in all life stages. There are limited morphological characteristics to differentiate the adult species, and it is nearly impossible to differentiate these two species in the egg and larval stages. Loop-mediated isothermal amplification (LAMP) is a rapid and robust diagnostic tool used to identify these two species accurately. We designed two sets of primers to distinguish B. minax and B. tsuneonis using DNA barcoding region of the COI gene. Only 50 min was needed under a constant temperature of 65ºC to determine the species of the two flies. The reaction system has high specificity and sensitivity, in which these two species can be accurately distinguished between different geographical populations and 1.0 ng/μL was the lowest DNA concentration that could be detected. Our primers can quickly identify these key pests without knowing their morphology, which could facilitate plant protection workers at the primary level to solve problems in plant quarantine.

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A loop-mediated isothermal amplification (LAMP) assay for rapid identification of Ceratitis capitata and related species

Cited by 6 publications

References 29 publications

Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Three Swimming Crab Species

Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Three Swimming Crab Species

Taxonomy and Biological Control: New Challenges in an Old Relationship

LAMP Assay as a Rapid Identification Technique of Chinese Citrus Fly and Japanese Orange Fly (Diptera: Tephritidae)

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