Simple and Efficient Method for Isolation and Measurement of Cyanobacterial Hepatotoxins by Plant Tests (Sinapis alba L.)

A laboratory study on risk assessment of microcystin-RR in cropland

“…The growth inhibitory phenomenon of MCs was first observed in white mustard (S. alba) seedlings (Ko´s et al, 1995). MCs were also found to inhibit photosynthesis of Phaseolus vulgaris primary leaves (Abe et al, 1996).…”

“…It is also intriguing to know the toxicological effects of MCs for different life stages of crop, since most of the previous reports of MCs' plant growth inhibitory effects were made with the sprouting plants only. (Ko´s et al, 1995;Kurki-Helasmo and Meriluoto, 1998;McElhiney et al, 2001;Chen et al, 2004).…”

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

“…Bioassays using mice [16] or plants (Sinapis alba L.) [17] have been used to screen for MCs, but were found to be non-specific or time-consuming. Biochemical methods such as enzyme-linked immunosorbent assay (ELISA) [18][19][20] and the protein phosphatase inhibition assay [5,21] are useful as screening methods due to their high sensitivity and high throughput, but they often have poor identification ability and the potential for false-positives.…”

Microcystin‐LR inhibits photosynthesis of Phaseolus vulgaris primary leaves: implications for current spray irrigation practice

“…Kos et al (1995) found inhibition of growth of mustard (Sinapis alba) seedlings, and used this response as an assay method for microcystin determination. Although microcystins were not evaluated, okadaic acid and calyculin-A arrested root hair growth and altered cortical cell shape in Arabidopsis thaliana L. seedlings (Smith et al, 1994).…”