Simple and Effective Regeneration of Mungbean (Vigna radiata (L) Wilczek) using Cotyledonary Node Explants

Vijayan, Smitha; Beena, M. R.; Kirti, P. B.

doi:10.1007/bf03321916

Cited by 15 publications

(10 citation statements)

References 14 publications

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“…The cultures initially incubated for 2 weeks in dark and subsequently transferred to 16 h photoperiod, showed higher rate of shoot multiplication as compared with the cultures incubated in completely 16 h photoperiod (data not shown). The cytokinin has significant effect on shoot multiplication as reported earlier in other legume crops like chickpea and mung bean [7,[18][19][20]. In the present study, the maximum (8.75) number of shoot buds/culture was obtained within 8 weeks culture on MS medium supplemented with 2 mg/l BAP ?…”

Section: Resultssupporting

confidence: 81%

Rapid In Vitro Plant Regeneration of Black Gram (Vigna mungo L. Hepper) Var. Sarala, an Important Legume Crop

Adlinge

Samal

Swamy

et al. 2013

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Efficient in vitro regeneration of black gram (Vigna mungo L. Hepper) var. Sarala was achieved through organogenesis using cotyledonary explants excised from 4 days old seedlings on MS medium supplemented with 2.0 mg/l BAP. Organogenic calli were developed from cotyledonary tissues within 4-6 weeks of culture on MS medium supplemented with 3.0 mg/l 6-benzylaminopurine (BAP) along with 2.0 mg/l 1-napthaleneacetic acid (NAA). Shoot bud regeneration was achieved on MS medium supplemented with 2.0 mg/l BAP within 3-4 weeks of subculture. The number of shoots per culture varied from 1.12 to 8.75 in different growth media. The cultures incubated initially on dark photoperiod for 2 weeks and subsequently transferred to 16 h photoperiod showed higher number of shoot bud regeneration. The proliferated shoots were further sub-cultured on similar medium for higher rate of shoot bud regeneration. The elongated shoots were rooted on strength MS medium fortified with 0.1-0.5 mg/l NAA or indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) with 2 % (w/v) sucrose within 2-3 weeks of culture. The higher percentage of rooting was obtained on 0.1-0.25 mg/l NAA as compared with IBA or IAA. The rooted plantlets were transferred to soil mixture (soil: sand: vermi-compost, 1:1:1 ratio) and kept in the greenhouse with 85 % humidity. The regenerated plantlets were successfully grown with 75 % survival rate. This protocol can be used for genetic improvement of black gram.

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Section: Resultssupporting

confidence: 81%

Rapid In Vitro Plant Regeneration of Black Gram (Vigna mungo L. Hepper) Var. Sarala, an Important Legume Crop

Adlinge

Samal

Swamy

et al. 2013

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…The co-cultivated explants were transferred to selection medium [basal medium of Murashige and Skoog (1962) supplemented with B 5 vitamins (Gamborg et al 1976)] containing 4.0 mg l -1 BAP (Vijayan et al 2006), 250 mg l -1 cefotaxime and 100 mg l -1 kanamycin. The explants were incubated at 28 ± 2°C under light intensity of *80 lEm -2 s -1 with 16 h photoperiod supplied by white fluorescent tubes.…”

Section: Tissue Culture and Genetic Transformationmentioning

confidence: 99%

“…Seeds of mungbean cultivar ML-267 were surface sterilized (Vijayan et al 2006). Cotyledonary nodes were excised from 2 day old seedlings and cocultivated with A. tumefaciens strain LBA4404 harboring BjNPR1 clone under the CaMV-35S promoter in the binary vector pCAMBIA2300 (Kesanakurti, unpublished) for 2-3 days.…”

Section: Explant Preparation and Transformationmentioning

confidence: 99%

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Mungbean plants expressing BjNPR1 exhibit enhanced resistance against the seedling rot pathogen, Rhizoctonia solani

Vijayan

Kirti

2011

Transgenic Res

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Mungbean, Vigna radiata (L.) Wilczek is an important pulse crop that is widely cultivated in semi- arid tropics. The crop is attacked by various soil-borne pathogens like Rhizoctonia solani, which causes dry rot disease and seriously affects its productivity. Earlier we characterized the non-expressor of pathogenesis related gene-1(BjNPR1) of mustard, Brassica juncea, the counterpart of AtNPR1 of Arabidopsis thaliana. Here, we transformed mungbean with BjNPR1 via Agrobacterium tumefaciens. Because of the recalcitrant nature of mungbean, the effect of some factors like Agrobacterium tumefaciens strains (GV2260 and LBA4404), pH, L: -cysteine and tobacco leaf extract was tested in transformation. The transgenic status of 15 plants was confirmed by PCR using primers for nptII. The independent integration of T-DNA in transgenic plants was analyzed by Southern hybridization with an nptII probe and the expression of BjNPR1 was confirmed by RT-PCR. Some of the T(0) plants were selected for detached leaf anti-fungal bioassay using the fungus Rhizoctonia solani, which showed moderate to high level of resistance depending on the level of expression of BjNPR1. The seedling bioassay of transgenic T(2) plants indicated resistance against dry rot disease caused by R. solani.

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“…Moreover, the appropriateness of this system toward abiotic stress resistance in leguminous plants is still unpracticed. Mung bean plants have been regenerated through, direct organogenesis by Chandra and Pal (1985), Mathew (1987), Gulati andJaiwal (1992. p. 94), Tivarekar andEapen (2001), Kumar et al (2003), Vijayan et al (2006), Mahalakshmi et al (2006), and Mundhara and Rashid (2006), and indirect organogenesis by Patel et al (1991), Mendoza et al (1992), Amutha et al (2003). However, the success of producing transgenics using these regeneration methods has been very low.…”

Section: Introductionmentioning

confidence: 99%

Transformation of Mungbean Plants for Salt and Drought Tolerance by introducing a gene for an osmoprotectant glycine betaine

Baloda¹,

Madanpotra²,

aiwal³

2017

JPSP

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Mung bean (Vigna radiata L. Wilczek) is a major grain legume extensively cultivated in equatorial and semitropical regions of the Indian subcontinent as well as in South East Asian countries. Protein and carbohydrate of mung bean are easily digestible and create less flatulence than proteins derived from other legumes. Mung bean is very sensitive to salty, and dessicated soil and variations of temperature (very low or very high), during the flowering and seed/pod development stages resulting in heavy losses to productivity. The development of plants by the addition and over expression of preferred abiotic stress tolerant genes through genetic transformation suggested to be a usable choice for obtaining abiotic stress tolerant plants. Stable transformation and expression of transgene (codA gene) was achieved in mung bean through Agrobacterium tumefaciens mediated system using cotyledonary node explants, under the optimized situations. The genomic tests of putative transgenic plants were done through polymerase chain reaction, dot-blot, enzyme-linked immunosorbent assay and Western blotting. The primary transformants were checked for salt tolerance by the leaf disc test. KEY WORDS: Abiotic stress, choline oxidase, glycine betaineOriginal Article Journal of Plant Stress Physiology 2017, 3: 5-11 http://scienceflora.org/journals/index.php/jpsp/ doi: 10.19071/jpsp.2017.v3.3148 Baloda, et al. Among different pathways of GB synthesis, the most suitable target for metabolic engineering of GB is COD pathway that changes choline into GB in only one step because it involves the transfer of a single gene codA.Engineering of GB biosynthesis in GB nonaccumulator leguminous crops including mung bean (Vigna radiata) are lacking in literature. Moreover, the appropriateness of this system toward abiotic stress resistance in leguminous plants is still unpracticed. Mung bean plants have been regenerated through, direct organogenesis by Chandra and Pal (1985), Mathew (1987), Gulati and Jaiwal (1992. p. 94 generated transgenics at a frequency of 2% using hpt gene. Sonia et al., (2007) developed transgenic plants of mung bean by transforming an insecticidal α-amylase inhibitor gene for bruchid resistance and the bar gene for herbicide resistance with a frequency of 1.15%.The current research was initiated with the aim to engineer the biosynthesis of GB into mung bean plants to intensify their resistance toward salinity and drought. MATERIALS AND METHODS TransformationThe Agrobacterium tumefaciens strain EHA101 carrying a binary vector pGAH/codA and cotyledonary node explants excised from 16 h old seedlings (presoaked in water) were used for the transformation studies. The explants after inoculated with bacterial strain were cultured on B 5 medium supplemented with 1.0 µm BAP, 75 mg/l kanamycin, 500 mgl −1 cefotaxime and 0.7% agar, for the regeneration of shoots. These explants were subcultured on a freshly prepared medium after every 2 weeks and maintained under the same conditions as for shoot regeneration. The green sho...

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Simple and Effective Regeneration of Mungbean (Vigna radiata (L) Wilczek) using Cotyledonary Node Explants

Cited by 15 publications

References 14 publications

Rapid In Vitro Plant Regeneration of Black Gram (Vigna mungo L. Hepper) Var. Sarala, an Important Legume Crop

Rapid In Vitro Plant Regeneration of Black Gram (Vigna mungo L. Hepper) Var. Sarala, an Important Legume Crop

Mungbean plants expressing BjNPR1 exhibit enhanced resistance against the seedling rot pathogen, Rhizoctonia solani

Transformation of Mungbean Plants for Salt and Drought Tolerance by introducing a gene for an osmoprotectant glycine betaine

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