Simple and effective label free electrochemical immunosensor for Fig mosaic virus detection

Haji-Hashemi, Hedieh; Safarnejad, Mohammad Reza; Norouzi, Parviz; Ebrahimi, Mostafa; Shahmirzaie, Morteza; Ganjali, Mohammad Reza

doi:10.1016/j.ab.2018.11.017

Cited by 43 publications

(24 citation statements)

References 20 publications

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“…Both types of immunosensors had been successfully used for the detection of particular viruses. Different viruses like , Plum pox virus ( Jarocka et al, 2013 ), DENV ( Darwish et al, 2015 ), Fig mosaic virus ( Haji-Hashemi et al, 2019 ) etc. are detected by electrochemical immunosensor recently.…”

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

“…A highly sensitive as well as selective label-free electrochemical immunosensors for the detection of Fig mosaic virus (FMV) has been developed with a LOD of 0.03 nM ( Haji-Hashemi et al, 2019 ). In this paper, a polyclonal antiserum (anti-FMV) against the virus nucleocapsid was immobilized at the surface of the gold electrode modified with 11-mercaptoundecanoic acid (MUA) and 3-mercapto propionic acid (MPA) through carbodiimide coupling reaction.…”

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

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Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

156

100

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Last few decades, viruses are a real menace to human safety. Therefore, the rapid identification of viruses should be one of the best ways to prevent an outbreak and important implications for medical healthcare. The recent outbreak of coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus which belongs to the single-stranded, positive-strand RNA viruses. The pandemic dimension spread of COVID-19 poses a severe threat to the health and lives of seven billion people worldwide. There is a growing urgency worldwide to establish a point-of-care device for the rapid detection of COVID-19 to prevent subsequent secondary spread. Therefore, the need for sensitive, selective, and rapid diagnostic devices plays a vital role in selecting appropriate treatments and to prevent the epidemics. During the last decade, electrochemical biosensors have emerged as reliable analytical devices and represent a new promising tool for the detection of different pathogenic viruses. This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods. Moreover, this review discusses different fabrication techniques, detection principles, and applications of various virus biosensors. Future research also looks at the use of electrochemical biosensors regarding a potential detection kit for the rapid identification of the COVID-19.

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Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

156

100

View full text Add to dashboard Cite

show abstract

“…63 Other immunosensor-based technologies have also been reported in recent times. [64][65][66] Experimental methods augmented with in silico approaches can significantly speed up the process of bio-probe development for tailored biosensing applications. Figure 7 shows the use of in silico approaches to the development of bio-probes, such as aptamers for SARS-CoV-2 N protein detection.…”

Section: Diagnosis Of Sars-cov-2 Based On In Silico Analysis Of Thementioning

confidence: 99%

“…For example, a piezoelectric immunosensor has been developed to detect SARS-CoV in sputum65 . Other immunosensor-based technologies have also been reported in recent times[66][67][68] . Experimental methods augmented with in silico approaches can significantly speed up the process of bio-probe development for tailored biosensing applications.…”

mentioning

confidence: 99%

Biophysical analysis of SARS‐CoV‐2 transmission and theranostic development via N protein computational characterization

Sabbih

Korsah

Jeevanandam

et al. 2020

Biotechnology Progress

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Recently, SARS-CoV-2 has been identified as the causative factor of viral infection called Covid-19, that belongs to the zoonotic beta coronavirus family known to cause respiratory disorders or viral pneumonia, followed by an extensive attack on organs that express angiotensin-converting enzyme II (ACE2). Human transmission of this virus occurs via respiratory droplets from symptomatic and asymptomatic patients, that are released into the environment after sneezing or coughing. These droplets are capable of staying in the air as aerosols or surfaces and can be transmitted to persons through inhalation or contact with contaminated surfaces. Thus, there is an urgent need for advanced theranostic solutions to control the spread of Covid-19 infection. The development of such fit-for-purpose technologies hinges on a proper understanding of the transmission, incubation and structural characteristics of the virus in the external environment as well as within the host. Hence, this article describes the development of an intrinsic model to describe the incubation characteristics of the virus under varying environmental factors. It also discusses on the evaluation of SARS-CoV-2 structural nucleocapsid protein properties via computational approaches to generate high-affinity binding probes for effective diagnosis and targeted treatment applications by specific targeting of viruses. In addition, this article provides useful insights on the transmission behavior of the virus and create new opportunities for theranostics development.

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“…Ultimately, researchers aim to develop a sensitive way to directly diagnose the original genetic target, compared with depending on amplified nucleic acids. For example, an immunosensor for fast and high sensitivity detection of a virus (e.g., Fig mosaic virus) was developed, relying on the antigen-antibody recognition [148]. The gold electrode surface was modified with the self-assembly monolayer of 11-mercaptoundecanoic acid and 3-mercapto propionic acid before immobilizing the biorecognition layer via the drop-casting method.…”

Section: Moving Toward Modern Electrochemical Detection Of Virusesmentioning

confidence: 99%

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

2020

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Personal biosensors and bioelectronics have been demonstrated for use in out-of-clinic biomedical devices. Such modern devices have the potential to transform traditional clinical analysis into a new approach, allowing patients or users to screen their own health or warning of diseases. Researchers aim to explore the opportunities of easy-to-wear and easy-to-carry sensors that would empower users to detect biomarkers, electrolytes, or pathogens at home in a rapid and easy way. This mobility would open the door for early diagnosis and personalized healthcare management to a wide audience. In this review, we focus on the recent progress made in modern electrochemical sensors, which holds promising potential to support point-of-care technologies. Key original research articles covered in this review are mainly experimental reports published from 2018 to 2020. Strategies for the detection of metabolites, ions, and viruses are updated in this article. The relevant challenges and opportunities of applying nanomaterials to support the fabrication of new electrochemical biosensors are also discussed. Finally, perspectives regarding potential benefits and current challenges of the technology are included. The growing area of personal biosensors is expected to push their application closer to a new phase of biomedical advancement.

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Simple and effective label free electrochemical immunosensor for Fig mosaic virus detection

Cited by 43 publications

References 20 publications

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Biophysical analysis of SARS‐CoV‐2 transmission and theranostic development via N protein computational characterization

Applying Nanomaterials to Modern Biomedical Electrochemical Detection of Metabolites, Electrolytes, and Pathogens

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