Simple and convenient method for culturing anaerobic bacteria

Abstract

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic incubation of broth cultures in Brewer Anaerobic Jars.

Anaerobic microorganisms in astrobiological analogue environments: from field site to culture collection

“…Methods to simplify enriching and isolating anaerobic microorganisms have been developed over a long period of time (e.g. M'Leod 1912;Kneteman 1957;Hungate 1969;Miller & Wolin 1974;Balch et al 1979;Behbehani et al 1982;La Scola et al 2014), but nevertheless they remain challenging since anaerobic organisms are fastidious and usually grow much more slowly than aerobic organisms. This may explain why although many anaerobic isolates have been obtained over the years from diverse environments, isolating such microorganisms from analogue environments specifically is less commonly attempted than aerobic organisms.…”

“…1979; Behbehani et al . 1982; La Scola et al . 2014), but nevertheless they remain challenging since anaerobic organisms are fastidious and usually grow much more slowly than aerobic organisms.…”