Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Depciuch, Joanna; Klębowski, Bartosz; Stec, Małgorzata; Szatanek, Rafał; Węglarczyk, Kazimierz; Baj‐Krzyworzeka, Monika; Parlińska-Wojtan, Magdalena; Baran, Jarosław

doi:10.3390/ijms21051826

Cited by 6 publications

(3 citation statements)

References 50 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As expected, the ER-associated calnexin was absent ( Figure 2 B). These data are in agreement with our previous studies, as well as studies by others [ 57 , 62 , 63 , 64 ]. The overexpression of CD9-GFP did not influence the presence or absence of these proteins in SW620 cell-derived EVs ( Figure 2 C).…”

Section: Resultssupporting

confidence: 94%

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells

Santos

Rappa

Fontana

et al. 2022

Cells

View full text Add to dashboard Cite

Intercellular communication between cancer cells themselves or with healthy cells in the tumor microenvironment and/or pre-metastatic sites plays an important role in cancer progression and metastasis. In addition to ligand–receptor signaling complexes, extracellular vesicles (EVs) are emerging as novel mediators of intercellular communication both in tissue homeostasis and in diseases such as cancer. EV-mediated transfer of molecular activities impacting morphological features and cell motility from highly metastatic SW620 cells to non-metastatic SW480 cells is a good in vitro example to illustrate the increased malignancy of colorectal cancer leading to its transformation and aggressive behavior. In an attempt to intercept the intercellular communication promoted by EVs, we recently developed a monovalent Fab fragment antibody directed against human CD9 tetraspanin and showed its effectiveness in blocking the internalization of melanoma cell-derived EVs and the nuclear transfer of their cargo proteins into recipient cells. Here, we employed the SW480/SW620 model to investigate the anti-cancer potential of the anti-CD9 Fab antibody. We first demonstrated that most EVs derived from SW620 cells contain CD9, making them potential targets. We then found that the anti-CD9 Fab antibody, but not the corresponding divalent antibody, prevented internalization of EVs from SW620 cells into SW480 cells, thereby inhibiting their phenotypic transformation, i.e., the change from a mesenchymal-like morphology to a rounded amoeboid-like shape with membrane blebbing, and thus preventing increased cell migration. Intercepting EV-mediated intercellular communication in the tumor niche with an anti-CD9 Fab antibody, combined with direct targeting of cancer cells, could lead to the development of new anti-cancer therapeutic strategies.

show abstract

Section: Resultssupporting

confidence: 94%

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells

Santos

Rappa

Fontana

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Among all the cancers, lung cancer is considered the second most diagnosed cancer, contributing to the highest mortality. , Lung cancer contributes 23% of the total cancer-related deaths in humans all over the world . Lung cancer is one of the common cancers all over the globe due to rapid progression, high incidence, and poor prognosis. , Small-cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) are the two types of lung cancer that make up 85% of the cases. NSCLC is regarded as a prominent and prevalent form of cancer. , In the Asian subcontinent, lung cancer exists as the potential reason for deaths and reduces the 5-year survival ratio by less than 15% .…”

Section: Introductionmentioning

confidence: 99%

“… 3 Lung cancer is one of the common cancers all over the globe due to rapid progression, high incidence, and poor prognosis. 4 , 5 Small-cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) are the two types of lung cancer that make up 85% of the cases. NSCLC is regarded as a prominent and prevalent form of cancer.…”

Section: Introductionmentioning

confidence: 99%

Apoptotic Potential and Antitumor Efficacy of Trilliumoside A: A New Steroidal Saponin Isolated from Rhizomes of Trillium govanianum

Tabassum,

Lone,

Bhat

et al. 2023

ACS Omega

View full text Add to dashboard Cite

Natural product-derived molecules exhibit potential as anticancer agents. Trilliumoside A, a new steroidal saponin, was obtained from rhizomes of Trillium govanianum, and its anticancer activity was investigated in the presented study. Trilliumoside A was investigated in a panel of cell lines, and it exhibited promising cytotoxic activity on the A549 cells (human lung cancer cells) with an IC 50 of 1.83 μM. The mechanism of cell death induced by Trilliumoside A in A549 cells and its anticancer potential in murine tumor models (EAC and EAT) were presented in the current research. Trilliumoside A was found to induce apoptosis in A549 cells by increasing the expression of various apoptotic proteins, such as Bax, Puma, cytochrome C, cleaved PARP, and cleaved caspase 3. Additionally, Trilliumoside A regulates the expression of p53, CDK2, and Cyclin A by decreasing the mitochondrial membrane potential, elevating reactive oxygen species, and stopping the growth of A549 cells in the synthesis phase (S) of the cell cycle. Trilliumoside A showed a considerable reduction in the tumor volume, the amount of ascitic fluid, and the total cell number without affecting the body weight of animals. Our results demonstrate that Trilliumoside A inhibits the proliferation of human lung cancer cells by inducing DNA damage, arresting the cell cycle, and activating the mitochondrial signaling pathway. The study demonstrated the potential of Trilliumoside A as a potential anticancer agent.

show abstract

Mechanical shear flow regulates the malignancy of colorectal cancer cells

Tseng,

Tsai,

Chen

et al. 2024

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

Colorectal cancer (CRC) is notable for its high mortality and high metastatic characteristics. The shear force generated by bloodstream provides mechanical signals regulating multiple responses of cells, including metastatic cancer cells, dispersing in blood vessels. We, therefore, studied the effect of shear flow on circulating CRC cells in the present study. The CRC cell line SW620 was subjected to shear flow of 12.5 dynes/cm2 for 1 and 2 h separately. Resulting elevated caspase‐9 and ‐3 indicated that shear flow initiated the apoptosis of SW620. Enlarged cell size associated with a higher level of cyclin D1 was coincident with the flow cytometric results indicating that the cell cycle was arrested at the G1 phase. An elevated phosphor‐eNOSS1177 increased the production of nitric oxide and led to reactive oxygen species‐mediated oxidative stress. Shear flow also regulated epithelial–mesenchymal transition (EMT) by increasing E‐cadherin and ZO‐1 while decreasing Snail and Twist1. The migration and invasion of sheared SW620 were also substantially decreased. Further investigations showed that mitochondrial membrane potential was significantly decreased, whereas mitochondrial mass and ATP production were not changed. In addition to the shear flow of 12.5 dynes/cm2, the expressions of EMT were compared at lower (6.25 dynes/cm2) and at higher (25 dynes/cm2) shear flow. The results showed that lower shear flow increased mesenchymal characteristics and higher shear flow increased epithelial characteristics. Shear flow reduces the malignancy of CRC in their metastatic dispersal that opens up new ways to improve cancer therapies by applying a mechanical shear flow device.

show abstract

Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Cited by 6 publications

References 50 publications

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells

Apoptotic Potential and Antitumor Efficacy of Trilliumoside A: A New Steroidal Saponin Isolated from Rhizomes of Trillium govanianum

Mechanical shear flow regulates the malignancy of colorectal cancer cells

Contact Info

Product

Resources

About