Simian virus 40 efficiently infects human T lymphocytes and extends their lifespan

Mazzoni, Elisa; Rigolin, Gian Matteo; Alaribe, Franca Nneka; Pancaldi, Cecilia; Maniero, Stefania; Comar, Manola; Martini, Fernanda; Tognon, Mauro

doi:10.1016/j.exphem.2012.02.008

Cited by 20 publications

(23 citation statements)

References 68 publications

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“…This observation is in line with the evidence that mesothelial cells [7], [8] immortalized fibroblasts [9] and T-lymphocytes [10] are only semi-permissive SV40 infection in vitro.…”

Section: Introductionsupporting

confidence: 89%

Serological Evidence of an Early Seroconversion to Simian Virus 40 in Healthy Children and Adolescents

et al. 2013

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At present Simian virus 40 (SV40) infection in humans appears to be transmitted independently from early contaminated vaccines. In order to test the spread of SV40 infection in children, an immunologic assay employing specific SV40 synthetic peptides corresponding to its viral protein (VP) antigens was employed to estimate the seroprevalence of this polyomavirus in Italian infants and adolescents. Serum samples from 328 children and adolescents, up to 17 years, were investigated. Serum antibodies against SV40 VPs were detected by indirect enzyme-linked immunosorbent assays. The seroprevalence of this polyomavirus was calculated after stratifying the subjects by age. Anti-viral capsid protein 1-2-3 SV40 IgG antibodies were detected in 16% of the study participants. The prevalence of antibodies against SV40 VPs tended to increase with age in children, up to 10 year old (21%). Then, in the cohort of individuals aged 11–17 years, the prevalence decreased (16%). A higher prevalence rate (23%) of SV40 VP antibodies was detected in the cohorts of 1–3 year and 7–10 year old children, than in children and adolescents of the other age groups. This age corresponds to children starting nursery and primary school, respectively, in Italy. IgM antibodies against SV40 VP mimotopes were detected in 6–8 month old children suggesting that SV40 seroconversion can occur early in life. SV40 VP antibodies are present at low prevalence in Italian children (16%), suggesting that SV40 infection, although acquired early in life, probably through different routes, is not widespread. The low SV40 seroprevalence suggests that SV40 is less transmissible than other common polyomaviruses, such as BKV and JCV. Alternatively, our immunologic data could be due to another, as yet undiscovered, human polyomavirus closely related to SV40.

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“…This observation is in line with the evidence that mesothelial cells [7], [8] immortalized fibroblasts [9] and T-lymphocytes [10] are only semi-permissive SV40 infection in vitro.…”

Section: Introductionsupporting

confidence: 89%

Serological Evidence of an Early Seroconversion to Simian Virus 40 in Healthy Children and Adolescents

et al. 2013

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“…Alternatively, SV40, although able with its Tag oncogene to bind and inactivate p53-and pRB-family proteins, could only be a passenger virus, multiplying better in some transformed cells than in normal cells. Indeed, it has been proved that normal human mesothelial cells as well as other normal human cells are only semipermissive to SV40 multiplication (49)(50)(51)(52). The high prevalence of SV40 antibodies in sera from MPM-affected patients is not proof of cause and effect in inducing human tumors by SV40.…”

Section: Discussionmentioning

confidence: 99%

High prevalence of serum antibodies reacting with simian virus 40 capsid protein mimotopes in patients affected by malignant pleural mesothelioma

Mazzoni¹,

Corallini

Cristaudo

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Human malignant pleural mesothelioma (MPM) is considered a rare tumor, but recent estimations indicate that one-quarter million people will die of this neoplasm in Europe in the next three decades. The mineral asbestos is considered the main causative agent of this neoplasm. MPM is largely unresponsive to conventional chemotherapy/radiotherapy. In addition to asbestos exposure, genetic predisposition to asbestos carcinogenesis and to simian virus (SV) 40 infection has also been suggested. SV40 is a DNA tumor virus found in some studies to be associated at high prevalence with MPM. SV40 sequences have also been detected, although at a lower prevalence than in MPM, in blood specimens from healthy donors. However, some studies have failed to reveal SV40 footprints in MPM and its association with this neoplasm. These conflicting results indicate the need for further investigations with new approaches. We report on the presence of antibodies in serum samples from patients affected by MPM that specifically react with two different SV40 mimotopes. The two SV40 peptides used in indirect ELISAs correspond to viral capsid proteins. ELISA with the two SV40 mimotopes gave overlapping results. Our data indicate that in serum samples from MPM-affected patients (n = 97), the prevalence of antibodies against SV40 viral capsid protein antigens is significantly higher (26%, P = 0.043) than in the control group (15%) represented by healthy subjects (n = 168) with the same median age (66 y) and sex. Our results suggest that SV40 is associated with a subset of MPM and circulates in humans.antibody | neoplasia

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“…Digital images were captured using ACT‐1 and ACT‐2 software for DXM1200F digital cameras (Nikon S.p.A., Florence, Italy). Nuclei were stained with 0.5 mg/mL 4,6′‐diamino‐2‐phenylindole (DAPI) . Digital images of cellular morphology were acquired with confocal microscope (LSM510; Carl Zeiss, Jena, Germany) using a 10 × 1.4 NA Plan‐Apochromat oil‐immersion objective and equipped with ZEN microscope imaging software (Zeiss Instruments).…”

Section: Methodsmentioning

confidence: 99%

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Mazzoni

D’Agostino

Iaquinta

et al. 2019

Stem Cells Translational Medicine

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100

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Tissue engineering‐based bone graft is an emerging viable treatment modality to repair and regenerate tissues damaged as a result of diseases or injuries. The structure and composition of scaffolds should modulate the classical osteogenic pathways in human stem cells. The osteoinductivity properties of the hydroxylapatite‐collagen hybrid scaffold named Coll/Pro Osteon 200 were investigated in an in vitro model of human adipose mesenchymal stem cells (hASCs), whereas the clinical evaluation was carried out in maxillofacial patients. Differentially expressed genes (DEGs) induced by the scaffold were analyzed using the Osteogenesis RT2 PCR Array. The osteoinductivity potential of the scaffold was also investigated by studying the alkaline phosphatase (ALP) activity, matrix mineralization, osteocalcin (OCN), and CLEC3B expression protein. Fifty patients who underwent zygomatic augmentation and bimaxillary osteotomy were evaluated clinically, radiologically, and histologically during a 3‐year follow‐up. Among DEGs, osteogenesis‐related genes, including BMP1/2, ALP, BGLAP, SP7, RUNX2, SPP1, and EGFR, which play important roles in osteogenesis, were found to be upregulated. The genes to cartilage condensation SOX9, BMPR1B, and osteoclast cells TNFSF11 were detected upregulated at every time point of the investigation. This scaffold has a high osteoinductivity revealed by the matrix mineralization, ALP activity, OCN, and CLEC3B expression proteins. Clinical evaluation evidences that the biomaterial promotes bone regrowth. Histological results of biopsy specimens from patients showed prominent ossification. Experimental data using the Coll/Pro Osteon 200 indicate that clinical evaluation of bone regrowth in patients, after scaffold implantation, was supported by DEGs implicated in skeletal development as shown in “in vitro” experiments with hASCs.

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Simian virus 40 efficiently infects human T lymphocytes and extends their lifespan

Cited by 20 publications

References 68 publications

Serological Evidence of an Early Seroconversion to Simian Virus 40 in Healthy Children and Adolescents

Serological Evidence of an Early Seroconversion to Simian Virus 40 in Healthy Children and Adolescents

High prevalence of serum antibodies reacting with simian virus 40 capsid protein mimotopes in patients affected by malignant pleural mesothelioma

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

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