Simian Virus 40 DNA Sequences in DNA of Healthy Adult Mice Derived from Preimplantation Blastocysts Injected with Viral DNA

Jaenisch, Rudolf; Mintz, Beatrice

doi:10.1073/pnas.71.4.1250

Cited by 362 publications

(147 citation statements)

References 20 publications

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“…2). In 1974, Rudolf Jaenisch generated the first transgenic mouse by retrovirus mediated infection of an early stage embryo, showing that this leads to integration of the foreign DNA into the host genome (Jaenisch and Mintz, 1974). Yet, it took until 1981 to show that injection of DNA into a pronucleus not only allows to engineer chimeric transgenic mice but also that this genetic material is passed on to subsequent generations (Brinster et al, 1981, Costantini and Lacy, 1981, Gordon and Ruddle, 1981.…”

Section: Technologies For the Generation Of Mouse Modelsmentioning

confidence: 99%

Modeling the Study of DNA Damage Responses in Mice

Specks¹,

Nieto-Soler²,

López-Contreras³

et al. 2015

Methods in Molecular Biology

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SummaryDamaged DNA has a profound impact on mammalian health and overall survival. In addition to being the source of mutations that initiate cancer, the accumulation of toxic amounts of DNA damage can cause severe developmental diseases and accelerate ageing. Therefore, understanding how cells respond to DNA damage has become one of the most intense areas of biomedical research in the recent years. However, whereas most mechanistic studies derive from in vitro or in cellulo work, the impact of a given mutation on a living organism is largely unpredictable. For instance, why BRCA1 mutations preferentially lead to breast cancer whereas mutations compromising mismatch repair drive colon cancer is still not understood. In this context, evaluating the specific physiological impact of mutations that compromise genome integrity has become crucial for a better dimensioning of our knowledge. We here describe the various technologies that can be used for modeling mutations in mice, and provide a review of the genes and pathways that have been modeled so far in the context of DNA damage responses.

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Section: Technologies For the Generation Of Mouse Modelsmentioning

confidence: 99%

Modeling the Study of DNA Damage Responses in Mice

Specks¹,

Nieto-Soler²,

López-Contreras³

et al. 2015

Methods in Molecular Biology

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“…The practicality of the strategy was demonstrated first in mammals (Jaenisch and Mintz, 1974;Jaenisch, 1976), but only recently in birds . However, these experiments relied on wildtype viruses and are therefore of value only under laboratory conditions.…”

Section: Methods For Gene Transfermentioning

confidence: 99%

Breeding for disease resistance ‐ the prospective role of genetic manipulation

Freeman

Bumstead

1987

Avian Pathology

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“…5 Biyomedikal amaçla geliştirilen ilk transgenik hayvan, aslında 1974 yılında viral bir DNA'nın erken dönem fare embriyosuna yerleştirilmesiyle elde edilmesine rağmen, farelerin bu transgeni döl-lerine geçirememesi nedeni ile uygulamada kullanım alanı bulamadığı bildirilmiştir. 6 Ancak, 1980 yılında pronükleer DNA mikroenjeksiyon yöntemi ile geliştirilen transgenik farelerin, genetik materyali ilk kez kendi döllerine de aktarabildiği göste-rilmiştir. 7 Bunun için insanlarda hastalığa neden olduğu bilinen gen alınarak (transgen veya aktarılan gen) hayvanlara rastgele yerleştirilir.…”

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