Simian Immunodeficiency Virus SIVmac239 Infection of Major Histocompatibility Complex-Identical Cynomolgus Macaques from Mauritius

Wiseman, Roger W.; Wojcechowskyj, Jason A.; Greene, Justin M.; Blasky, Alex J.; Gopon, Tobias; Soma, Taeko; Friedrich, Thomas C.; O’Connor, Shelby L.; O’Connor, David H.

doi:10.1128/jvi.01841-06

Cited by 155 publications

(256 citation statements)

References 45 publications

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“…Here, vaccinated macaques with H3 or H6 haplotypes exhibited higher peak proliferative responses to Tat prior to challenge, suggesting that cellular immunity may contribute to the resistant phenotype. The remarkably simple MHC genetics of the geographically isolated Mauritian cynomolgus population [37] can be exploited in prospective studies to further explore the relationship between MHC haplotypes, immune response, and susceptibility to infection with SHIV 89.6P and other SHIV isolates or SIV strains.…”

Section: Discussionmentioning

confidence: 99%

“…Microsatellite PCR assays were performed with genomic DNAs and a panel of 16 markers spanning the 5-Mb MHC region essentially as described previously [37,53]. MHC haplotype predictions were generated based on the microsatellite profiles, inferring alleles for the class I and class II regions based on previously established haplotype-allele associations [36,37].…”

Section: Mhc Microsatellite Haplotype Analysismentioning

confidence: 99%

“…MHC haplotype predictions were generated based on the microsatellite profiles, inferring alleles for the class I and class II regions based on previously established haplotype-allele associations [36,37].…”

Section: Mhc Microsatellite Haplotype Analysismentioning

confidence: 99%

“…The characterization of six common MHC haplotypes in feral Mauritian cynomolgus macaques [37] allowed determination of MHC genotypes for the cynomolgus macaques studied here. Microsatellite allele profiles were used to infer haplotypes spanning the 5-Mb MHC region and deduce complete genotypes for class I and class II alleles (Fig.…”

Section: Analysis Of Mhc Haplotypesmentioning

confidence: 99%

“…As the entire repertoire of MHC alleles can now be predicted for essentially all Mauritian cynomolgus macaques [35][36][37], we also determined the MHC genotypes of the study animals. These investigations revealed a new association of Mauritian MHC haplotype and susceptibility/resistance to SHIV 89.6P infection.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

Florese

Wiseman

Venzon

et al. 2008

Vaccine

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Protection afforded by HIV Tat-based vaccines has differed in Indian rhesus and Mauritian cynomolgus macaques. We evaluated native Tat and Ad-HIVtat priming/Tat-boosting regimens in both species. Both vaccines were immunogenic. Only the Ad-tat regimen modestly reduced acute viremia in rhesus macaques after SHIV 89.6P challenge. Confounding variables uncovered in Mauritian macaques included significant associations of susceptibility to infection with MHC class IB and class II H2 and H5 haplotypes, and resistance to infection with class IB haplotypes H3 and H6. Although protection here was limited, Tat-based vaccines incorporating other HIV components have shown greater efficacy. Combination strategies should be further explored.

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Section: Discussionmentioning

confidence: 99%

Section: Mhc Microsatellite Haplotype Analysismentioning

confidence: 99%

Section: Mhc Microsatellite Haplotype Analysismentioning

confidence: 99%

Section: Analysis Of Mhc Haplotypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

Florese

Wiseman

Venzon

et al. 2008

Vaccine

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Microsatellite Variation in Two Subspecies of Cynomolgus Monkeys (Macaca fascicularis)

Sun

Zeng

et al. 2012

American J Primatol

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To estimate the genetic variability of two subspecies of cynomolgus monkeys (Macaca fascicularis fascicularis and M. f. aurea) using microsatellite markers, 26 microsatellite markers were selected from previous reports. Seventeen markers showed high polymorphism in a subset of monkeys and were used for the assessment of genetic diversity in the larger sample. The effective number of alleles, the polymorphism information content (PIC) and the expected heterozygosity of M. f. aurea monkeys were all statistically significantly higher than those of M. f. fascicularis monkeys (P < 0.05), suggesting the M. f. aurea monkeys had a higher degree of genetic variation than the M. f. fascicularis monkeys. Substantial differences in allele distribution were also detected between the two subspecies of cynomolgus monkeys. Private alleles restricted to the M. f. fascicularis or the M. f. aurea monkeys were found throughout the selected 17 loci. These private alleles may allow the discrimination of the two subspecies of cynomolgus monkeys. The selected markers could also be used to estimate the genetic variation for other subspecies of cynomolgus monkeys. Further work using additional animals obtained from native or independent sources will be important for a more complete understanding of the genetic differences between these two subgroups.

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Identification of MhcMafa‐DRB Alleles in a Cohort of Cynomolgus Macaques of Vietnamese Origin

Wang

Ling

et al. 2012

American J Primatol

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Cynomolgus macaques have been used widely to build a research model of infectious and chronic diseases, as well as in transplantation studies, where disease susceptibility and/or resistance are associated with the major histocompatibility complex (MHC). To better elucidate polymorphisms and genetic differences in the Mafa-DRB locus, and facilitate the experimental use of cynomolgus macaques, we used pool screening combined with cloning and direct sequencing of polymerase chain reaction products to characterize MhcMafa-DRB gene alleles in 153 Vietnamese cynomolgus macaques. We identified 30 Mafa-DRB alleles belonging to 17 allelic lineages, including four novel sequences that had not been documented in earlier reports. The highest frequency allele was Mafa-DRB*W27:04, which was present in 7 of 35 (20%) monkeys. The next most frequent alleles were Mafa-DRB*3:07 and Mafa-DRB*W7:01, which were detected in 5 of 35 (14.3%) and 4 of 35 (11.4%) of the monkeys, respectively. The high-frequency alleles in this Vietnamese population may be high priority targets for additional characterization of immune functions. Only the DRB1*03 and DRB1*10 lineages were also present in humans, whereas the remaining alleles were monkey-specific lineages. We found 25 variable sites by aligning the deduced amino acid sequences of 29 identified alleles. Evolutionary and population analyses based on these sequences showed that human, rhesus, and cynomolgus macaques share several Mhc-DRB lineages and the shared polymorphisms in the DRB region may be attributable to the existence of interbreeding between rhesus and cynomolgus macaques. This information will promote the understanding of MHC diversity and polymorphism in cynomolgus macaques and increase the value of this species as a model for biomedical research.

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Simian Immunodeficiency Virus SIVmac239 Infection of Major Histocompatibility Complex-Identical Cynomolgus Macaques from Mauritius

Cited by 155 publications

References 45 publications

Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

Microsatellite Variation in Two Subspecies of Cynomolgus Monkeys (Macaca fascicularis)

Identification of MhcMafa‐DRB Alleles in a Cohort of Cynomolgus Macaques of Vietnamese Origin

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