Silymarin prevents iron overload induced bone loss by inhibiting oxidative stress in an ovariectomized animal model

Tao, Zhou-Shan; Li, Tianlin; Wei, Shan

doi:10.1016/j.cbi.2022.110168

Cited by 25 publications

(14 citation statements)

References 38 publications

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“…However, treatment with silymarin reduced oxidative stress in the serum of these rats. Therefore, the results of this study are consistent with our study, and silymarin was able to reduce oxidative stress by decreasing MDA and increasing SOD2 levels [53].…”

Section: Discussionsupporting

confidence: 92%

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The Role of Silymarin in Mitigating Inflammation and Cognitive Impairment Induced by Ovariectomy in Wistar Rats

Moalefshahri,

Javid,

Gheybi

et al. 2023

Mediators of Inflammation

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Background. Silymarin, a polyphenolic flavonoid found in milk thistle, has been used to treat liver and brain injuries in humans and animals. The study aims to investigate the protective effects of silymarin on spatial and passive avoidance memory, oxidative stress, and inflammatory factors in the brain and liver tissues of ovariectomized (OVX) Wistar rats. Methods. The study involved 30 female Wistar rats divided into control, sham, and three silymarin-treated groups. After ovariectomy, rats underwent CT scan, and some of them were administered silymarin via gavage for 2 months. Memory and learning were assessed using Morris water maze and shuttle box tests. Brain and liver tissues were analyzed for inflammatory factors (IL-1β, TNFα, and IL-6) and oxidative stress markers (CAT, SOD, and MDA) after sacrifice. Results. Silymarin improved spatial memory and fear learning compared to the sham group (P≤0.05 to P≤0.001). It also significantly reduced IL-1β, TNF-α, and IL-6 levels in the cortex, hippocampus, and liver (P≤0.05 to P≤0.0001) and increased CAT and SOD while decreasing MDA levels (P≤0.05 to P≤0.001) compared to control and sham groups. Conclusion. Long-term administration of silymarin extract can improve learning and memory, reverse cognitive impairment caused by ovariectomy, and reduce oxidative stress and inflammatory factors induced by ovariectomy in the liver and brain of Wistar rats. This is due to the reduction in MDA levels and an increase in CAT activity, although silymarin has some effect on SOD at high doses.

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Section: Discussionsupporting

confidence: 92%

“…A study by Tao et al [53] showed that administration of ammonium ferric citrate to OVX rats resulted in decreased levels of SOD2 and total antioxidant capacity and increased levels of MDA in serum. However, treatment with silymarin reduced oxidative stress in the serum of these rats.…”

Section: Discussionmentioning

confidence: 99%

The Role of Silymarin in Mitigating Inflammation and Cognitive Impairment Induced by Ovariectomy in Wistar Rats

Moalefshahri,

Javid,

Gheybi

et al. 2023

Mediators of Inflammation

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“…These reactions have attracted much attention due to their ability to produce complex polycyclic structures ( e.g. natural products, pharmaceuticals, or valuable intermediates) 11–55 (Scheme 1).…”

Section: From Classical Cycloadditions To Higher-order Cycloadditionsmentioning

confidence: 99%

Recent advances in metal-free catalytic enantioselective higher-order cycloadditions

Zhang,

Wang

2024

Org. Chem. Front.

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“…Subsequently, staining with HE and Masson techniques was performed as per the established protocols. [28][29][30] To visualize the newly formed bone tissue within the damaged femur. The visualization process involved capturing images using a biomicroscope (Ni-E, Nikon, Japan).…”

Section: Histological Evaluationmentioning

confidence: 99%

“…The expression of Osteocalcin (OC) and Tartrate-resistant acid phosphatase (TRAP) in bone tissue of defect area was further investigated by examining bone sections prepared for histological analysis according to previous reports. 29,31 Briefly, sections were deparaffinized, rehydrated, and repaired by 0.05% trypsin at 37°C for 30 min. Incubation with anti-OC (1:200, Boster Bio) were performed at 4°C for 10 h. DAB Kit (ZSGB-BIO Corporation, Beijing, China) was used to develop positive expression.…”

Section: Immunofluorescent Analysismentioning

confidence: 99%

Favorable osteogenic activity of vericiguat doped in β-tricalcium phosphate: In vitro and in vivo studies

Tao,

Shen

2024

J Biomater Appl

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Recently, more and more studies have shown that guanylate cyclase, an enzyme that synthesizes cyclic guanosine monophosphate (cGMP), plays an important role in bone metabolism. Vericiguat (VIT), a novel oral soluble guanylate cyclase stimulator, directly generates cyclic guanosine monophosphate and reduce the death incidence from cardio-vascular causes or hospitalization. Recent studies have shown beneficial effects of VIT in animal models of osteoporosis, but very little is currently known about the effects of VIT on bone defects in the osteoporotic states. Therefore, in this study, β-tricalcium phosphate (β-TCP) was used as a carrier to explore the effect of local VIT administration on the repair of femoral metaphyseal bone defects in ovariectomized (OVX) rats. When MC3T3-E1 was cultured in the presence of H2H2, VIT, similar to Melatonin (MT), therapy could increase the matrix mineralization and ALP, SOD2, SIRT1, and OPG expression, reduce ROS and Mito SOX production, RANKL expression, Promote the recovery of mitochondrial membrane potential. In the OVX rat model, VIT increases the osteogenic effect of β-TCP and better results were obtained at a dose of 5 mg. Local use of VIT can inhibit increased OC, BMP2 and RUNX2 expressions in bone tissue, while decreased SOST and TRAP expressions by RT-PCR and immunohistochemistry. Thereby, VIT stimulates bone regeneration and is a promising candidate for promoting bone repair in osteoporosis.

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Silymarin prevents iron overload induced bone loss by inhibiting oxidative stress in an ovariectomized animal model

Cited by 25 publications

References 38 publications

The Role of Silymarin in Mitigating Inflammation and Cognitive Impairment Induced by Ovariectomy in Wistar Rats

The Role of Silymarin in Mitigating Inflammation and Cognitive Impairment Induced by Ovariectomy in Wistar Rats

Recent advances in metal-free catalytic enantioselective higher-order cycloadditions

Favorable osteogenic activity of vericiguat doped in β-tricalcium phosphate: In vitro and in vivo studies

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