Silver Nanostructures: Limited Sensitivity of Detection, Toxicity and Anti-Inflammation Effects

Морозова, О. В.

doi:10.3390/ijms22189928

Cited by 20 publications

(17 citation statements)

References 36 publications

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“…First, silver ions can cause depolarization of the cellular membrane due to electrostatic interactions with membrane phospholipids and proteins, resulting in destabilization of the cytomembrane. 29 Notably, AgNPs have unique antibacterial mechanisms that are totally different from those of other AgBMs. Direct contact with uncharged nanoparticles can also rupture the bacterial cell membrane as a result of the physiochemical features of nanoparticles, such as surface topology, size, geometry and hydrophobicity, regardless of germ type.…”

Section: Characteristics and Performance Of Agbmsmentioning

confidence: 99%

Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in Dentistry

Wang¹,

Zhang²,

Li³

et al. 2022

IJN

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Microbial infection accounts for many dental diseases and treatment failure. Therefore, the antibacterial properties of dental biomaterials are of great importance to the long-term results of treatment. Silver-based biomaterials (AgBMs) have been widely researched as antimicrobial materials with high efficiency and relatively low toxicity. AgBMs have a broad spectrum of antimicrobial properties, including penetration of microbial cell membranes, damage to genetic material, contact killing, and dysfunction of bacterial proteins and enzymes. In particular, advances in nanotechnology have improved the application value of AgBMs. Hence, in many subspecialties of dentistry, AgBMs have been researched and employed, such as caries arresting or prevention, root canal sterilization, periodontal plaque inhibition, additives in dentures, coating of implants and anti-inflammatory material in oral and maxillofacial surgery. This paper aims to provide an overview of the application approaches of AgBMs in dentistry and present better guidance for oral antimicrobial therapy via the development of AgBMs.

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Section: Characteristics and Performance Of Agbmsmentioning

confidence: 99%

Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in Dentistry

Wang¹,

Zhang²,

Li³

et al. 2022

IJN

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“…Nanosilver may be applied for disinfection, inactivated vaccine production, and combined therapy without a risk of pathogen resistance due to the multiple mechanisms of action [ 1 , 3 ] and allergic complications for hosts because of nanosilver-induced immunosupression [ 20 , 21 ]. Cellular uptake of nanosilver may mainly induce T-helper 1 cellular immune response necessary for elimination of intracellular pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…Protein surface shells protected Ag NPs from dissolution and aggregation. Therefore, the nanoconjugates of the noble metal NPs with proteins remained stable for several months at 4 °C [ 5 , 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…The cytotoxicity of Ag nanoconjugates with IgG essentially exceeded that of AgNO 3 ( Table 1 ), perhaps due to the cellular uptake of silver nanostructures covered with IgG but not Ag + ions. The subsequent “Trojan horse” results in high Ag + intracellular concentration and interaction with proteins, nucleic acids, and membranes [ 1 , 3 , 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…Ag NPs exhibit antiviral activity against influenza A virus [ 8 , 9 , 10 , 11 , 12 ], respiratory syncytial virus [ 13 , 14 ], hepatitis B virus [ 15 ], monkeypox virus [ 16 ], vaccinia virus [ 17 ], human parainfluenza virus [ 18 ], herpes simplex virus [ 6 , 18 ], and human immunodeficiency virus (HIV) type 1 [ 19 ]. Despite extensive research and proven antiviral properties, the implementation of nanosilver in commercial antiviral drugs is limited [ 2 , 20 , 21 , 22 ]. Cytotoxicity is a major limitation for their use as antiviral agents in biomedical applications [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins

Морозова

Manuvera

Grishchechkin

et al. 2022

Viruses

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Nanosilver possesses antiviral, antibacterial, anti-inflammatory, anti-angiogenesis, antiplatelet, and anticancer properties. The development of disinfectants, inactivated vaccines, and combined etiotropic and immunomodulation therapy against respiratory viral infections, including COVID-19, remains urgent. Aim: Our goal was to determine the SARS-CoV-2 molecular targets (genomic RNA and the structural virion proteins S and N) for silver-containing nanomaterials. Methods: SARS-CoV-2 gene cloning, purification of S2 and N recombinant proteins, viral RNA isolation from patients’ blood samples, reverse transcription with quantitative real-time PCR ((RT)2-PCR), ELISA, and multiplex immunofluorescent analysis with magnetic beads (xMAP) for detection of 17 inflammation markers. Results: Fluorescent Ag nanoclusters (NCs) less than 2 nm with a few recovered silver atoms, citrate coated Ag nanoparticles (NPs) with diameters of 20–120 nm, and nanoconjugates of 50–150 nm consisting of Ag NPs with different protein envelopes were constructed from AgNO3 and analyzed by means of transmission electron microscopy (TEM), atomic force microscopy (AFM), ultraviolet-visible light absorption, and fluorescent spectroscopy. SARS-CoV-2 RNA isolated from COVID-19 patients’ blood samples was completely cleaved with the artificial RNase complex compound Li+[Ag+2Cys2−(OH−)2(NH3)2] (Ag-2S), whereas other Ag-containing materials provided partial RNA degradation only. Treatment of the SARS-CoV-2 S2 and N recombinant antigens with AgNO3 and Ag NPs inhibited their binding with specific polyclonal antibodies, as shown by ELISA. Fluorescent Ag NCs with albumin or immunoglobulins, Ag-2S complex, and nanoconjugates of Ag NPs with protein shells had no effect on the interaction between coronavirus recombinant antigens and antibodies. Reduced production of a majority of the 17 inflammation biomarkers after treatment of three human cell lines with nanosilver was demonstrated by xMAP. Conclusion: The antiviral properties of the silver nanomaterials against SARS-CoV-2 coronavirus differed. The small-molecular-weight artificial RNase Ag-2Sprovided exhaustive RNA destruction but could not bind with the SARS-CoV-2 recombinant antigens. On the contrary, Ag+ ions and Ag NPs interacted with the SARS-CoV-2 recombinant antigens N and S but were less efficient at performing viral RNA cleavage. One should note that SARS-CoV-2 RNA was more stable than MS2 phage RNA. The isolated RNA of both the MS2 phage and SARS-CoV-2 were more degradable than the MS2 phage and coronavirus particles in patients’ blood, due to the protection with structural proteins. To reduce the risk of the virus resistance, a combined treatment of Ag-2S with Ag NPs could be used. To prevent cytokine storm during the early stages of respiratory infections with RNA-containing viruses, nanoconjugates of Ag NPs with surface proteins could be used.

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Improved Anticoagulant Effect of Layered Double Hydroxide‐Heparin Nanocomposites on the Microcirculation of Septic Rats

Mao

Shan

Wang

et al. 2022

Advanced Therapeutics

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Anticoagulant therapy is an important therapeutic tool for septic microcirculatory dysfunction. Low molecular weight heparin (LMWH) is a potent anticoagulant with antithrombotic properties, which is a crucial factor for sepsis treatment. However, it has a short plasma half-life and achieves low local concentrations; nanocomposite formulations may help overcome these limitations. Herein, it is aimed to determine if a functional layered double hydroxide (LDH)-LMWH nanocomposite improves microcirculation and organ function, while maximizing the antithrombotic effects and reducing bleeding risks in septic rats. This nanocomposite (fluorescein isothiocyanate (FITC)-fibrin antibody (Ab)-bovine serum albumin (BSA)-20LMWH-LDH) is constructed by intercalating LMWH and FITC into the LDH interlayer, and application of a BSA and an Ab coating onto the LDH nanoparticle surface. The nanocomposite released LMWH in a gradual and biphasic pattern, in an acidity-triggered manner. Capillary tubule studies and intestinal microcirculation investigations show that green fluorescent nanocomposites accumulated around microthrombi, validating their targeting ability. Animal experiments reveal that the nanocomposites significantly improve microcirculation in septic rats with minimal pathological damage to the heart, lung, liver, and kidney tissues. Thus, this study provides a novel strategy for the treatment of septic microcirculatory dysfunction in clinical situations.

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Silver Nanostructures: Limited Sensitivity of Detection, Toxicity and Anti-Inflammation Effects

Cited by 20 publications

References 36 publications

Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in Dentistry

Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in Dentistry

Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins

Improved Anticoagulant Effect of Layered Double Hydroxide‐Heparin Nanocomposites on the Microcirculation of Septic Rats

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