Silver nanoparticles synthesized with Eucalyptus critriodora ethanol leaf extract stimulate antibacterial activity against clinically multidrug-resistant Acinetobacter baumannii isolated from pneumonia patients

Wintachai, Phitchayapak; Paosen, Supakit; Yupanqui, Chutha Takahashi; Voravuthikunchai, Supayang Piyawan

doi:10.1016/j.micpath.2018.11.018

Cited by 58 publications

(32 citation statements)

References 32 publications

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“…Studies have also indicated the possible prevention and eradication of biofilm-associated infections with NAg. Wintachai et al (2019) reported over 90% inhibition of viable MDR A. baumannii (NPRCOE 160575) at low NAg doses (0.09 μg/ml or 0.5x the reported MIC), which prevented the attachment and subsequent biofilm formation of (media-suspended) A. baumannii on the surface of human lung epithelia (cell line A549). Indeed, the team observed negligible toxicity of the nanoparticle towards to the lung cells (50% cytotoxicity concentration [CC 50 ] = 5.72 μg/ml) which is important when considering the medical application of NAg (Wintachai et al, 2019).…”

Section: Effect Of Nag On a Baumannii And Other Bacterial Biofilmsmentioning

confidence: 93%

“…Wintachai et al (2019) reported over 90% inhibition of viable MDR A. baumannii (NPRCOE 160575) at low NAg doses (0.09 μg/ml or 0.5x the reported MIC), which prevented the attachment and subsequent biofilm formation of (media-suspended) A. baumannii on the surface of human lung epithelia (cell line A549). Indeed, the team observed negligible toxicity of the nanoparticle towards to the lung cells (50% cytotoxicity concentration [CC 50 ] = 5.72 μg/ml) which is important when considering the medical application of NAg (Wintachai et al, 2019). Singh et al (2018) reported a greater extent of eradication of A. baumannii biofilms with NAg [minimum biofilm eradication concentration (MBEC) = 2 mg/ml] when compared to tetracycline, erythromycin, and doxycycline, citing extensive EPS destruction and reduction in viable cells following nanoparticle treatment.…”

Section: Effect Of Nag On a Baumannii And Other Bacterial Biofilmsmentioning

confidence: 93%

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Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

et al. 2021

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The misuse of antibiotics combined with a lack of newly developed ones is the main contributors to the current antibiotic resistance crisis. There is a dire need for new and alternative antibacterial options and nanotechnology could be a solution. Metal-based nanoparticles, particularly silver nanoparticles (NAg), have garnered widespread popularity due to their unique physicochemical properties and broad-spectrum antibacterial activity. Consequently, NAg has seen extensive incorporation in many types of products across the healthcare and consumer market. Despite clear evidence of the strong antibacterial efficacy of NAg, studies have raised concerns over the development of silver-resistant bacteria. Resistance to cationic silver (Ag+) has been recognised for many years, but it has recently been found that bacterial resistance to NAg is also possible. It is also understood that exposure of bacteria to toxic heavy metals like silver can induce the emergence of antibiotic resistance through the process of co-selection. Acinetobacter baumannii is a Gram-negative coccobacillus and opportunistic nosocomial bacterial pathogen. It was recently listed as the “number one” critical level priority pathogen because of the significant rise of antibiotic resistance in this species. NAg has proven bactericidal activity towards A. baumannii, even against strains that display multi-drug resistance. However, despite ample evidence of heavy metal (including silver; Ag+) resistance in this bacterium, combined with reports of heavy metal-driven co-selection of antibiotic resistance, little research has been dedicated to assessing the potential for NAg resistance development in A. baumannii. This is worrisome, as the increasingly indiscriminate use of NAg could promote the development of silver resistance in this species, like what has occurred with antibiotics.

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Section: Effect Of Nag On a Baumannii And Other Bacterial Biofilmsmentioning

confidence: 93%

Section: Effect Of Nag On a Baumannii And Other Bacterial Biofilmsmentioning

confidence: 93%

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

et al. 2021

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“…To evaluate the anti-ESBL-KP biofilm potential, the effect of phage on biofilm formation by ESBL-KP was determined by the co-incubation of phage and bacteria at 37 °C without agitation for 24 and 48 h as described previously 66 , 79 – 82 . Briefly, ESBL-KP in TSB were added into a flat-bottomed 96-well microtiter plate (Thermo Fisher Scientific Inc., Waltham, MA) at concentrations of 10 8 CFU/mL in 100 μL.…”

Section: Methodsmentioning

confidence: 99%

Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo

Wintachai

Naknaen

Thammaphet

et al. 2020

Sci Rep

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Extended spectrum β lactamase-producing Klebsiella pneumoniae (ESBL-KP) is being reported with high morbidity and mortality rates and is considered as the highest priority for new antimicrobial strategies. To develop an alternative antimicrobial agent, phage KP1801 with broad lytic activity was isolated. The genome of phage KP1801 was double stranded DNA of 49,835 base pairs, with a GC content of 50.26%. There were 75 putative open reading frames. Phage KP1801 was classified as being in the order Caudovirales , belonging to the Siphoviridae family. About 323 proteins were detected by shotgun proteome analysis. The phage inhibited biofilm formation and reduced pre-formed biofilm in a dose dependent manner. Scanning electron microscopic studies demonstrated a membrane damage of bacterial cells treated with phage, resulting in cell death. Prophylactic and therapeutic efficacies of the phage were evaluated in Galleria mellonella . Administration of ESBL-KP infection with phage significantly improved the survival of G. mellonella . The number of intracellular bacteria in larvae showed a significant decrease compared with untreated control while the number of phage increased. These studies suggested that phage KP1801 has the potential for development as an alternative for antibiotics and biocontrol agents against ESBL-KP infection.

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“…The MIC and MBC values of Fer@AgNP against A. baumannii were more compelling than those obtained in other experiments using other plant extracts or living microorganisms from different plants or living organisms (Neurada procumbens [44], Xanthomonas spp. [45], Eucalyptus citriodora [46], and Acinetobacter calcoaceticus) to synthesize biogenic AgNPs. The bacterial growth was inhibited at a concentration range from 20 μg/mL to 500 μg/mL.…”

Section: Antibacterial Effects Against a Baumanniimentioning

confidence: 99%

Antibacterial Effects of Green-Synthesized Silver Nanoparticles Using Ferula asafoetida against Acinetobacter baumannii Isolated from the Hospital Environment and Assessment of Their Cytotoxicity on the Human Cell Lines

Abootalebi

Mousavi

Hashemi

et al. 2021

Journal of Nanomaterials

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Acinetobacter baumannii (A. baumannii) is a dangerous nosocomial pathogen in intensive care units, causing fatal clinical challenges and mortality. In this study, the green synthesis of silver nanoparticles (AgNPs) using the extract of Ferula asafetida and the chemical synthesis of AgNPs were carried out to evaluate their effects on A. baumannii bacterial strain and a human adenocarcinoma cell line. The NPs were characterized using several techniques, including field emission-scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectrometry, UV-visible spectroscopy, and Fourier-transform infrared spectroscopy. After synthesis, the arrangement of AgNPs was confirmed based on the maximum absorption peak at 450 nm. The results showed that the AgNPs had a hexagonal structure. The antimicrobial activity of biogenic NPs significantly increased and reached a minimum inhibitory concentration of 2 μg/mL. The nanomaterials did not exhibit any toxic effects on the human cell line at certain concentrations and showed improvements compared to chemically synthesized AgNPs. However, at higher concentrations (100 μg/mL), the cytotoxicity increased. Finally, it was concluded that biosynthesized AgNPs had significant antimicrobial effects on A. baumannii isolated from intensive care units.

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Silver nanoparticles synthesized with Eucalyptus critriodora ethanol leaf extract stimulate antibacterial activity against clinically multidrug-resistant Acinetobacter baumannii isolated from pneumonia patients

Cited by 58 publications

References 32 publications

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo

Antibacterial Effects of Green-Synthesized Silver Nanoparticles Using Ferula asafoetida against Acinetobacter baumannii Isolated from the Hospital Environment and Assessment of Their Cytotoxicity on the Human Cell Lines

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