Silver nanoparticles obtained from Brazilian pepper extracts with synergistic anti-microbial effect: production, characterization, hydrogel formulation, cell viability, and <i>in vitro</i> efficacy

Oliveira, Daniele M de; Menezes, Diego Batista; Andrade, Lucas Rannier Melo de; Lima, Felipe da C; Hollanda, Luciana Maria de; Zielińska, Aleksandra; Souto, Eliana B.; Severino, Patrícia

doi:10.1080/10837450.2021.1898634

Cited by 21 publications

(17 citation statements)

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“…The dynamic polysaccharides’ nature is highly diverse, due to their nature, composition, and chemical [ 19 ] interactions. Hydrogels are produced by several gelling methods (physical, thermal, and ionic); however, chemical methods provide a controlled crosslinking, resulting in modifications, as they can affect the polymer biofunctionality [ 20 , 21 , 22 ]. According to this, we present some chemical characteristics of plant-derived polysaccharides and gelling behavior ( Table 1 ) to better comprehend the engineered plant polysaccharide gels.…”

Section: Plant-derived Polymersmentioning

confidence: 99%

Plant Polysaccharides in Engineered Pharmaceutical Gels

et al. 2022

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Hydrogels are a great ally in the pharmaceutical and biomedical areas. They have a three-dimensional polymeric structure that allows the swelling of aqueous fluids, acting as an absorbent, or encapsulating bioactive agents for controlled drug release. Interestingly, plants are a source of biogels, specifically polysaccharides, composed of sugar monomers. The crosslinking of these polymeric chains forms an architecture similar to the extracellular matrix, enhancing the biocompatibility of such materials. Moreover, the rich hydroxyl monomers promote a hydrophilic behavior for these plant-derived polysaccharide gels, enabling their biodegradability and antimicrobial effects. From an economic point of view, such biogels help the circular economy, as a green material can be obtained with a low cost of production. As regards the bio aspect, it is astonishingly attractive since the raw materials (polysaccharides from plants-cellulose, hemicelluloses, lignin, inulin, pectin, starch, guar, and cashew gums, etc.) might be produced sustainably. Such properties make viable the applications of these biogels in contact with the human body, especially incorporating drugs for controlled release. In this context, this review describes some sources of plant-derived polysaccharide gels, their biological function, main methods for extraction, remarkable applications, and properties in the health field.

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Section: Plant-derived Polymersmentioning

confidence: 99%

Plant Polysaccharides in Engineered Pharmaceutical Gels

et al. 2022

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“…The term "nanoparticle" refers to nanosized materials (usually between 0 and 100 nm) that have three dimensions (3D). The use of silver for the development of nanoparticles increased initially because of its biomedical applications, mainly as an effective antimicrobial agent [3,19]. Over the years, with the improvement of studies in relation to nanomaterials, it was possible to recognize several possibilities of application for silver nanoparticles (Ag-NPs), such in the development of food packaging to protect against microorganisms [20,21], the production of biosensors [22,23], and water decontamination [24,25].…”

Section: Silver Nanoparticles: General Features and Synthesis Approachesmentioning

confidence: 99%

“…It can be applied for several purposes, such as to promote improvements in human and animal health, create more durable consumer goods, increase agricultural and industrial productivity [1,2]. As a result, nanoparticles have been used for different goals, as a method to deliver fertilizers, in the development of antimicrobials against bacterial resistance, as environmental monitoring biosensors, and for pest control [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

et al. 2021

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Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.

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“…Studies have revealed that geraniol has several pharmacological properties, such as antifungal, anti-inflammatory, antimicrobial, repellent, and insecticidal activities [ 14 , 15 , 16 ]. However, the use of geraniol is limited by factors such as light, oxygen, humidity, and high temperatures, leading to its degradation or the generation of toxic substances, even inhibiting its repellent activity [ 17 ]. Among several substances, geraniol and icaridin are the main actives present in repellents marketed and recommended by international health authorities for being considered cosmetically more pleasant to use than DEET.…”

Section: Introductionmentioning

confidence: 99%

Polymeric Nanorepellent Systems Containing Geraniol and Icaridin Aimed at Repelling Aedes aegypti

Andrade

Guilger-Casagrande

Germano-Costa

et al. 2022

IJMS

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Repellents are among the leading products used against diseases transmitted by the Aedes aegypti mosquito. However, their indiscriminate use or high concentrations can cause severe adverse reactions, particularly in children and pregnant women. To protect them, nanotechnology is a promising tool to encapsulate active compounds against degradation, increase their effectiveness, and decrease their toxicity, as it can promote the modified release of the active compound. This study aimed to develop polymeric nanocapsules containing the repellent actives geraniol and icaridin using low concentrations of the active component, with the objective of promoting effective activity and greater safety against adverse reactions. The nanocapsules were developed by the interfacial deposition method, and the physicochemical properties of the nanocapsules were evaluated using dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), zeta potential, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), release kinetics assay, and mathematical modeling. Cell viability was assessed by the MTT assay and genotoxicity analysis using the comet assay. The developed nanocapsules containing geraniol and icaridin showed mean diameters of 260 nm and 314 nm, respectively, with a polydispersity index < 0.2. The nanocapsules showed encapsulation efficiency values of 73.7 ± 0.1% for icaridin and 98.7 ± 0.1% for geraniol. Morphological analysis showed spherical nanocapsules with low polydispersity. The kinetic parameters calculated using the Korsmeyer–Peppas model indicated an anomalous release profile. Cell viability and genotoxicity analyses showed that the nanocapsules did not alter cell viability or damage DNA. The results demonstrate a promising nanostructured system with good physicochemical characteristics and good stability, with repellent activity against Aedes aegypti.

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Silver nanoparticles obtained from Brazilian pepper extracts with synergistic anti-microbial effect: production, characterization, hydrogel formulation, cell viability, and in vitro efficacy

Cited by 21 publications

References 50 publications

Plant Polysaccharides in Engineered Pharmaceutical Gels

Plant Polysaccharides in Engineered Pharmaceutical Gels

Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

Polymeric Nanorepellent Systems Containing Geraniol and Icaridin Aimed at Repelling Aedes aegypti

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