Silver Nanoparticles Mediate Differential Responses in Keratinocytes and Fibroblasts during Skin Wound Healing

Abstract

With advances in nanotechnology, pure silver has been recently engineered into nanometer-sized particles (diameter <100 nm) for use in the treatment of wounds. In conjunction with other studies, we previously demonstrated that the topical application of silver nanoparticles (AgNPs) can promote wound healing through the modulation of cytokines. Nonetheless, the question as to whether AgNPs can affect various skin cell types--keratinocytes and fibroblasts--during the wound-healing process still remains. Therefore, the aim of this study was to focus on the cellular response and events of dermal contraction and epidermal re-epithelialization during wound healing under the influence of AgNPs; for this we used a full-thickness excisional wound model in mice. The wounds were treated with either AgNPs or control with silver sulfadiazine, and the proliferation and biological events of keratinocytes and fibroblasts during healing were studied. Our results confirm that AgNPs can increase the rate of wound closure. On one hand, this was achieved through the promotion of proliferation and migration of keratinocytes. On the other hand, AgNPs can drive the differentiation of fibroblasts into myofibroblasts, thereby promoting wound contraction. These findings further extend our current knowledge of AgNPs in biological and cellular events and also have significant implications for the treatment of wounds in the clinical setting.

Therapeutic effect of Aloe vera and silver nanoparticles on acid-induced oral ulcer in gamma-irradiated mice

“…5 AV and AgNPs alone or in combination increased the number of activated fibroblasts and mature vascular endothelial cells at the 3rd day. This result is in agreement with Liu et al, 36 who reported that AgNPs enhanced fibroblast transformation into myofibroblast. Similarly, Liu et al, 37 found that AV increased fibroblast proliferation and collagen synthesis, and elevated hyaluronic acid and hydroxyproline level, which suggest a possible role in the extracellular matrix remodeling during wound healing.…”

Biosynthesis of silver nanoparticles by endophytic fungi: Its mechanism, characterization techniques and antimicrobial potential

“…According to Sibbald et al (2007) the use of silver nanoparticles dressing on a variety of chronic non-healing wounds has a beneficial effect of protecting the wound site from bacterial contamination. Similarly, Liu et al (2010) proved that silver nanoparticles could promote wound healing through facilitating the proliferation and migration of keratinocyte.…”

Bigger Size Silver Nanoparticles Hamper Mother and Fetus Nutrition after Serial Ingestion of Bolotic Colloidal Nano Silver Stirring Solution through Oral Route: A Revolutionary Research Discovery in Teratogenicity & Toxicity Field

“…Fact based prime level research propounded that large size AgNps causes chondrosomic respiratory chain autophagy and for that regenerate oxygen sensory genus tension [2][3][4][5][6][7][8][9]. Vertebrate primate affecting bacterial stem cells are evidenced to be disrupted by bigger size AgNps and have the capability to arise demolition of reproductive, digestion activities [10]. Still today some specific in-vivo experiments done and the results showed bigger size AgNps have very serious impacts on human being like mal nutrition, atrophy and respiratory ailments which may lead to fatal condition [11,12] this category particles also evokes oxygen sensory genus tension with programmed cell death consequence [13][14][15] and essential amino acids with proteins demarcated at hypovalemic shock stage due to malnutrition [16].…”