Silver Nanoparticles for a Colorimetric Determination of Putrescine and Cadaverine in Biological Samples

Abstract

A convenient and uncomplicated scheme has been projected for the quantitative determination of essential diamine putrescine (PUT) and cadaverine (CAD) via sodium dodecyl sulfate protected silver nanoparticles (SDS-AgNPs). The scheme is based on the chemical interaction of SDS-AgNPs probe with PUT and CAD leading to the color change from yellow to red or reddish brown. The interaction was investigated through different techniques such as Uv-visible spectrophotometer, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectroscopy (EDX), Dynamic Light Scattering Spectroscopy (DLS) and Zeta potential. Both the amines posses close resemblance in structure (except for addition of one more methylene group in CAD), no any distinguishable color change was noted. Whereas, maximum absorption band at 580 nm and 600 nm was demonstrated for PUT and CAD correspondingly. The methodical response was observed at the absorption ratio of 580/410 and 600/410 nm, with the linear regression within 4-12 µg/mL and 6-14 µg/mL for PUT and CAD. The detection limit calculated for both the diamine PUT and CAD was 0.333 µg/mL and 1.638 µg/mL. The scheme was applied successfully for the Analytical Sciences

A novel fluorescent probe of alkyne compounds for putrescine detection based on click reaction

“…The LOD of DAB is 3.19 Â 10 À7 mol L À1 , which is better than those of many reported putrescine probes (Table 2). 24,[34][35][36] In the national food safety standard, the detection limit of DAB in wine and vinegar is about 5.7 Â 10 À5 mol L À1 , so it can be applied to the detection of actual samples.…”

“…However, this detection method is only faster at 80 C. 23 Khuhawar et al used sodium lauryl sulfate to protect silver nanoparticles, allowing silver nanoparticles to interact with putrescine and cadaverine, and established a quantitative detection method for putrescine and cadaverine. 24 Sathiyanarayanan et al developed a new donor-acceptor (D-A) type ratiometric and colorimetric sensor using a pyridine ring and biphenyl dicyandiethylene as the donor and acceptor, respectively, which can selectively and efficiently identify biogenic primary amines. 25 However, most uorescence sensors need specic conditions such as high temperature or catalyst, which limits the uorescence sensors in the detection of putrescine.…”