Silver nanoparticles elicited
            <i>in vitro</i>
            callus cultures for accumulation of biomass and secondary metabolites in
            <i>Caralluma tuberculata</i>

Ali, Amir; Mohammad, Sher; Khan, Mubarak Ali; Raja, Naveed Iqbal; Arif, Mohammad; Kamil, Atif; Mashwani, Zia‐ur‐Rehman

doi:10.1080/21691401.2019.1577884

Cited by 120 publications

(95 citation statements)

References 42 publications

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“…Employment of ENPs in the tissue/cell culture industry of medicinal plants is limited despite a lot of progress having been made in the last decade in the agricultural nanotechnology sector. The available literature suggests that ENPs evoke abiotic stress in tissue/cell culture raised medicinal plants [43][44][45][46][47][48]. These studies illustrate the effects induced by ENPs on the growth and secondary metabolism of plants of medicinal significance.…”

Section: Discussionmentioning

confidence: 92%

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

et al. 2020

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Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.

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Section: Discussionmentioning

confidence: 92%

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

et al. 2020

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“…PAL content in the cultured shoot was determined by the protocol of Ali et al [ 72 ]. About 80–100 mg of each powder shoot sample was mixed with 100 mM potassium borate (ice cold buffer of pH 8.8) then added 2 nM of mercaptoethanol to the mixture.…”

Section: Methodsmentioning

confidence: 99%

Micropropagation and Production of Health Promoting Lignans in Linum usitatissimum

Khan

Shehzad

et al. 2020

Plants

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Linum usitatissimum commonly known as flax or linseed is an important medicinal plant, produces medicinally potent lignans, used in the treatment of several human diseases. Lignans limited production in the natural plants does not meet the increasing market demand. This study was conducted to establish an easy and rapid method for the in vitro micropropagation and production of potent lignans and antioxidant secondary metabolites in linseed. The results indicated that hypocotyl explants under the effects of thidiazuron (TDZ: 0.5 mg/L) + kinetin (Kn: 0.5 mg/L) in the basal growth media, resulted in the optimal shoot organogenesis parameters (shoot induction frequency: 86.87%, number of shoots: 6.3 ± 0.36 and shoots length: 6.5 ± 0.54 cm), in 4 weeks. Further, TDZ supplementation in the culture media efficiently activated the antioxidant system in the in vitro raised shoots, wherein maximum production of total phenolic content, TPC (34.33 ± 0.20 mg of GAE/g DW); total flavonoid content, TFC (8.99 ± 0.02 mg of QE/g DW); DPPH free radical scavenging activity (92.7 ± 1.32%); phenylalanine ammonia-lyase activity, PAL (8.99 ± 0.02 U/g FW); and superoxide dismutase expression, SOD (3.62 ± 0.01 nM/min/mg FW) were observed in the shoot cultures raised in presence of TDZ: 0.5 mg/L + Kn: 0.5 mg/L. Nonetheless, considerable levels of pharmacologically active lignans such as secoisolariciresinol (SECO: 23.13–37.10 mg/g DW), secoisolariciresinol diglucoside (SDG: 3.32–3.86 mg/g DW) and anhydrosecoisolariciresinol diglucoside (ANHSECO: 5.15–7.94 mg/g DW) were accumulated in the regenerated shoots. This protocol can be scaled up for the commercial production of linseed to meet the market demands for lignans.

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“…Plant growth regulators have been employed along with different MNPs to check their combinative effect on elicitation of SMs in various plant species. 28,51,52 In combination with plant growth regulators (TDZ and 2,4-D), AgNP elicitation of bitter gourd (Momordica charantia) cell culture resulted in an enhanced production of phenolic, hydroxycinnamic and hydroxybenzoic acids, and avonoids. 53 Enhanced production of stevia glycosides was observed in callus culture of Stevia rebaudiana Bertoni simultaneously elicited with AgNPs and salicylic acid.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

“…Although a number of reports suggested various signaling pathways to explain the modulation capacity of nanomaterials on the production of plant SMs, to date the precise mechanism still remains elusive. 10,51,84,109 Probably, because elicitation mechanisms are very complex, and could involve thousands of mediators from multiple signaling pathways and their crosstalk. Additionally, all these events uctuate depending on the origin, specicity, exposure type, time and concentration of elicitors along with the plant dependent parameter such as the plant species developmental stage, cellular cycle, type of tissue, nutritional conditions, elicitor uptake by media/soil/aerial, physiochemical environment and so on.…”

Section: Postulated Mechanism Of Elicitation Of Specialized Metabolitmentioning

confidence: 99%

Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: current status and future outlooks

Anjum

Hano

et al. 2019

RSC Adv.

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Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata

Abstract: ur-Rehman Mashwani (2019) Silver nanoparticles elicited invitro callus cultures for accumulation of biomass and secondary metabolites in

Cited by 120 publications

References 42 publications

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

Micropropagation and Production of Health Promoting Lignans in Linum usitatissimum

Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: current status and future outlooks

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