Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

Çiftçi, Hakan; Türk, Mustafa; Tamer, Uğur; Karahan, Siyami; Menemen, Yusuf

doi:10.3906/biy-1302-21

Cited by 58 publications

(14 citation statements)

References 47 publications

(57 reference statements)

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“…In another study, the toxic effect of Ag-NPs on the MCF-7 cell line was also evaluated. Cellular cytotoxicity was observed from a nanoparticle concentration of 10 µg/mL [90]. This is opening the door to the use of this nanomaterial against cancer cells and therefore, to be a possible cancer therapy, alone or in combination with other existing methods [86,94].…”

Section: In Vitro Studies: Static and Dynamic Gut Simulators And Epitmentioning

confidence: 87%

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

et al. 2020

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Foodborne diseases are one of the factors that endanger the health of consumers, especially in people at risk of exclusion and in developing countries. The continuing search for effective antimicrobials to be used in the food industry has resulted in the emergence of nanotechnology in this area. Silver nanoparticles (Ag-NPs) are the nanomaterial with the best antimicrobial activity and therefore, with great potential of application in food processing and packing. However, possible health effects must be properly addressed to ensure food safety. This review presents a detailed description on the main applications of Ag-NPs as antimicrobial agents for food control, as well as the current legislation concerning these materials. Current knowledge about the impact of the dietary exposure to Ag-NPs in human health with special emphasis on the changes that nanoparticles undergo after passing through the gastrointestinal tract and how they alter the oral and gut microbiota, is also summarized. It is concluded that given their potential and wide properties against foodborne pathogens, research in Ag-NPs is of great interest but is not exempt from difficulties that must be resolved in order to certify the safety of their use.

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Section: In Vitro Studies: Static and Dynamic Gut Simulators And Epitmentioning

confidence: 87%

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

et al. 2020

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“…This concentration of AgNPs usually causes a decrease in cell viability by ~30% (Çiftçi et al, 2013; Kreeinthong and Uawithya, 2014). At this concentration, the cells once treated with AgNPs may recover growth if transferred to fresh medium devoid of AgNPs.…”

Section: Agnps Exposure To Human Cells and Tissues: Implications Of Cmentioning

confidence: 99%

“…In particular, redox-sensitive mitogen-activated protein kinase (MAPK) pathway, and transcription factors such as AP-1, Nrf-2, and nuclear factor kappa-B (NF-κB) are known to play important role (Lee et al, 2009; Manke et al, 2013). At intermediate oxidative stress level, cells viability reduces below 50% upon treatment with 40 μg/mL AgNPs (Çiftçi et al, 2013). Based on experimental data, at this concentration cells show maximum apoptotic effect (Çiftçi et al, 2013), which may be attributed to both AgNPs-induce cytotoxicity (mitochondrial dysfunction) and AgNPs-mediated DNA damage and genotoxicity (cell cycle arrest in G2/M phase) (Asharani et al, 2009a).…”

Section: Agnps Exposure To Human Cells and Tissues: Implications Of Cmentioning

confidence: 99%

“…At intermediate oxidative stress level, cells viability reduces below 50% upon treatment with 40 μg/mL AgNPs (Çiftçi et al, 2013). Based on experimental data, at this concentration cells show maximum apoptotic effect (Çiftçi et al, 2013), which may be attributed to both AgNPs-induce cytotoxicity (mitochondrial dysfunction) and AgNPs-mediated DNA damage and genotoxicity (cell cycle arrest in G2/M phase) (Asharani et al, 2009a). In fact, in vitro studies conducted on a number of other cell lines such as pulmonary fibroblasts, epithelial cells, melanoma cells, and hepatoma cells showed that AgNPs at a concentration of 13.45 μg/mL results in significant increase in membrane leakage of LDH and reduction in mitochondrial function (Ávalos Fúnez et al, 2013).…”

Section: Agnps Exposure To Human Cells and Tissues: Implications Of Cmentioning

confidence: 99%

“…The high oxidative stress condition caused by 50 μg/mL AgNPs or more in RAW264.7 cells and MCF-7 showed 70% reduction in cell viability (Çiftçi et al, 2013; Paul et al, 2015). Çiftçi et al (2013) also found that at high AgNPs concentration, the apoptotic affect reduces, while the necrotic effect becomes prominent. This effect can be attributed oxidative stress induced damages to mitochondrial membrane and dysfunction of electron chain dysfunction, which ultimately lead to necrotic cell death.…”

Section: Agnps Exposure To Human Cells and Tissues: Implications Of Cmentioning

confidence: 99%

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Mechanistic Basis of Antimicrobial Actions of Silver Nanoparticles

et al. 2016

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Multidrug resistance of the pathogenic microorganisms to the antimicrobial drugs has become a major impediment toward successful diagnosis and management of infectious diseases. Recent advancements in nanotechnology-based medicines have opened new horizons for combating multidrug resistance in microorganisms. In particular, the use of silver nanoparticles (AgNPs) as a potent antibacterial agent has received much attention. The most critical physico-chemical parameters that affect the antimicrobial potential of AgNPs include size, shape, surface charge, concentration and colloidal state. AgNPs exhibits their antimicrobial potential through multifaceted mechanisms. AgNPs adhesion to microbial cells, penetration inside the cells, ROS and free radical generation, and modulation of microbial signal transduction pathways have been recognized as the most prominent modes of antimicrobial action. On the other side, AgNPs exposure to human cells induces cytotoxicity, genotoxicity, and inflammatory response in human cells in a cell-type dependent manner. This has raised concerns regarding use of AgNPs in therapeutics and drug delivery. We have summarized the emerging endeavors that address current challenges in relation to safe use of AgNPs in therapeutics and drug delivery platforms. Based on research done so far, we believe that AgNPs can be engineered so as to increase their efficacy, stability, specificity, biosafety and biocompatibility. In this regard, three perspectives research directions have been suggested that include (1) synthesizing AgNPs with controlled physico-chemical properties, (2) examining microbial development of resistance toward AgNPs, and (3) ascertaining the susceptibility of cytoxicity, genotoxicity, and inflammatory response to human cells upon AgNPs exposure.

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p(thyme oil) and p(clove oil) organo‐particles with biocompatible, anticancer, antioxidant, and antibacterial properties against Capan‐1 and L‐929 cells

et al. 2023

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The synthesis of p(ClO) and p(TO) organo‐particles from clove oil and thyme oil is the first in the literature. The particles were tested against the L‐929 cell line for cell viability/cytotoxicity. The anticancer activity was studied against the Capan‐1 pancreatic cancer cell line. p(ClO) and p(TO) organo‐particles were featured by thermogravimetry (TGA), Fourier transform infrared spectroscopy (FT‐IR), scanning electron microscopy (SEM), particle size (DLS), and particle charge (zeta potential, Zeta) analyses. Antioxidant, biocompatible, antimicrobial, and in vitro cytotoxicity specialties were investigated. p(ClO) and p(TO) organo‐particles were found to be effective on the L‐929 fibroblast cell line and Capan‐1 pancreatic cancer cell line in research on Capan‐1 and L‐929 cell lines. Additionally, it was shown that large dosages of p(ClO) organo‐particles were not hazardous to L‐929 cell lines. A difference was found between the rates of cell viability and apoptosis and necrosis when the MTT study findings of p(ClO) and p(TO) organo‐particles were studied in Capan‐1 cell line. The p(TO) organo‐particle had the highest % apoptosis rate. At the 100 g mL−1 concentration, the fibroblast cell viability of p(ClO) and p(TO) organo‐particles was 176.46% and 107.78%, respectively. The IC50 value derived for the decrease in viability was determined as (2.22 mg mL−1) and it was calculated that it would kill the pancreatic cancer cells by 50% when doxorubicin and p(ClO) were administered combined.

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Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

Abstract: doi:10.3906/biy-1302-2

Cited by 58 publications

References 47 publications

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

Mechanistic Basis of Antimicrobial Actions of Silver Nanoparticles

p(thyme oil) and p(clove oil) organo‐particles with biocompatible, anticancer, antioxidant, and antibacterial properties against Capan‐1 and L‐929 cells

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