In this work, thin films made from the blends of collagen and chitosan with silver nanoparticles were prepared and their properties, which are important for potential biomedical applications, were studied. Thin biopolimeric film was obtained by solvent evaporation from collagen/chitosan mixture containing silver nanoparticles. Mechanical properties of the composites were tested in dry condition using a mechanical testing machine. Microstructure of films was analyzed by Atomic Force Microscope. The surface properties were analyzed by contact angle measurements using hydrophilic and hydrophobic liquids. In addition, antimicrobial properties of collagen/chitosan/silver composites were tested during the microbiological test. It was found that the addition of silver nanoparticles to collagen/chitosan film affects several of its properties. Microbiological studies showed that obtained collagen/chitosan/silver nanoparticles materials possess bacteriostatic activity against Staphylococcus aureus.