Silver nanoparticles anchored on nitrogen-doped graphene as a novel electrochemical biosensing platform with enhanced sensitivity for aptamer-based pesticide assay

Abstract

Silver nanoparticles (NPs) decorated nitrogen doped graphene (NG) nanocomposites were prepared through a one-step thermal-treatment route using arginine as the nitrogen source. By integrating the excellent electrical properties and large surface area of Ag NPs and NG, the obtained Ag/NG nanocomposites show more effective electron transfer and high loading capacity than Ag-graphene and pure NG. In the presence of a target, the stronger interaction between the aptamer and the target promotes the formation of a target-aptamer complex on the electrode surface which blocks the electron transfer. Based on this sensing mechanism, a novel and highly sensitive biosensing platform by the use of Ag/NG as enhancing materials is demonstrated for detection of the model target, acetamiprid. The presented aptasensor exhibited a wide linear response for acetamiprid in the range of 1 × 10(-13) M to 5 × 10(-9) M with a low detection limit of 3.3 × 10(-14) M (S/N = 3). Moreover, this electrochemical aptasensor avoided complicated labeling procedures and showed magnificent sensitivity, high selectivity and low cost, which made it not only convenient but also time-saving and applicable. Furthermore, the proposed design may offer a promising way to develop a new electrochemical aptasensor for sensitive and specific detection of a wide spectrum of analytes in food, medical and environmental fields.

Boron doped graphene wrapped silver nanowires as an efficient electrocatalyst for molecular oxygen reduction

“…This insertion prevents the restacking of BG. The peak broadening and the increased d-spacing of C (002) peak can also be attributed to the more interaction of AgNWs with the BG sheets, which is in excellent agreement with the literature reports4950. For example, Jiang et al .…”

“…For example, Jiang et al . reported that the XRD spectrum of silver nanoparticles incorporated nitrogen doped graphene exhibit broad reflection at ∼26° corresponds to C (002) peak49. Zhao et al .…”

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

“…Functionalization of the electrodes with high-affinity biomolecules enhances besides selectivity and also the sensitivity of the system. Therefore, EIS is very often combined with different nanostructured interfaces in order to increase the amount of biorecognition material on the surface and therefore to improve the sensor sensitivity and extend its linear working [54][55][56][57][58][59]. This improvement is associated with the dimensions of nanomaterials, which endows them with a large surface/volume ratio and high specific area enabling to immobilize bigger amount of biomolecules onto biosensor surface [60].…”

“…Jiang et al proposed an aptamer-based biosensor for the detection of acetamiprid [59]. To increase the sensitivity of the system silver nanoparticles, decorated nitrogen-doped graphene (NG) nanocomposites were used.…”

Affinity Sensing Strategies for the Detection of Pesticides in Food

“…This, in theory, should amplify the response signals. Accordingly, an impedimetric aptasensor based on AgNPs decorated nitrogen doped graphene (NG) nanocomposite has been proposed by Jiang et al [ 99 ]. The presented aptasensor exhibited a broad linear range from 1.00 × 10 −13 M to 5.00 × 10 −9 M with an extremely low detection limit of 3.30 × 10 −14 M. Spiked cucumber and tomato samples were analyzed with a recovery range of 86.4–109%.…”