Silver nanoparticle‐induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues

Kim, Soohee; Ryu, Doug-Young

doi:10.1002/jat.2792

Cited by 329 publications

(231 citation statements)

References 74 publications

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“…Generation of reactive oxygen species (ROS) by the Ag NPs has also been considered a primary mode of cytotoxic action of Ag NPs [22,29]. Several studies observed a high level of ROS in cells treated with Ag NPs [30][31][32][33].…”

Section: Role Of Agmentioning

confidence: 99%

Antibacterial activity of silver nanoparticles: A surface science insight

2015

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Summary Silver nanoparticles constitute a very promising approach for the development of new antimicrobial systems. Nanoparticulate objects can bring significant improvements in the antibacterial activity of this element, through specific effect such as an adsorption at bacterial surfaces. However, the mechanism of action is essentially driven by the oxidative dissolution of the nanoparticles, as indicated by recent direct observations. The role of Ag + release in the action mechanism was also indirectly observed in numerous studies, and explains the sensitivity of the antimicrobial activity to the presence of some chemical species, notably halides and sulfides which form insoluble salts with Ag + . As such, surface properties of Ag nanoparticles have a crucial impact on their potency, as they influence both physical (aggregation, affinity for bacterial membrane, etc.) and chemical (dissolution, passivation, etc.) phenomena. Here, we review the main parameters that will affect the surface state of Ag NPs and their influence on antimicrobial efficacy. We also provide an analysis of several works on Ag NPs activity, observed through the scope of an oxidative Ag + release.

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Section: Role Of Agmentioning

confidence: 99%

Antibacterial activity of silver nanoparticles: A surface science insight

2015

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“…Oxidative stress in turn plays an important role in many types of cellular injury, which can result in DNA damage and apoptotic cell death. [63][64][65][66][67][68] ROS are chemical species that are produced as by-products of cellular oxygen metabolism, which occurs via mitochondrial respiration in eukaryotic cells. [63][64][65][66][67][68] AgNPs are capable of producing ROS.…”

mentioning

confidence: 99%

“…Abnormal accumulation of ROS is called oxidative stress, and can lead to serious cellular damage. [63][64][65][66][67][68][69][70][71] According to a literature survey, currently there is limited information available about the mechanism of AgNP-induced toxicity. To know the effect of AgNPs on oxidative stress, we measured ROS generation using the H 2 DCF-DA assay.…”

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confidence: 99%

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

Gurunathan¹,

Raman²,

Malek³

et al. 2013

IJN

126

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Background Silver nanoparticles (AgNPs) are an important class of nanomaterial for a wide range of industrial and biomedical applications. AgNPs have been used as antimicrobial and disinfectant agents due their detrimental effect on target cells. The aim of our study was to determine the cytotoxic effects of biologically synthesized AgNPs using hot aqueous extracts of the mycelia of Ganoderma neo-japonicum Imazeki on MDA-MB-231 human breast cancer cells. Methods We developed a green method for the synthesis of water-soluble AgNPs by treating silver ions with hot aqueous extract of the mycelia of G. neo-japonicum. The formation of AgNPs was characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction, dynamic light scattering, and transmission electron microscopy. Furthermore, the toxicity of synthesized AgNPs was evaluated using a series of assays: such as cell viability, lactate dehydrogenase leakage, reactive oxygen species generation, caspase 3, DNA laddering, and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling in human breast cancer cells (MDA-MB-231). Results The ultraviolet-visible absorption spectroscopy results showed a strong resonance centered on the surface of AgNPs at 420 nm. The X-ray diffraction analysis confirmed that the synthesized AgNPs were single-crystalline, corresponding with the result of transmission electron microscopy. Treatment of MDA-MB-231 breast cancer cells with various concentrations of AgNPs (1–10 μg/mL) for 24 hours revealed that AgNPs could inhibit cell viability and induce membrane leakage in a dose-dependent manner. Cells exposed to AgNPs showed increased reactive oxygen species and hydroxyl radical production. Furthermore, the apoptotic effects of AgNPs were confirmed by activation of caspase 3 and DNA nuclear fragmentation. Conclusion The results indicate that AgNPs possess cytotoxic effects with apoptotic features and suggest that the reactive oxygen species generated by AgNPs have a significant role in apoptosis. The present findings suggest that AgNPs could contribute to the development of a suitable anticancer drug, which may lead to the development of a novel nanomedicine for the treatment of cancers.

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“…Fact based prime level research propounded that large size AgNps causes chondrosomic respiratory chain autophagy and for that regenerate oxygen sensory genus tension [2][3][4][5][6][7][8][9]. Vertebrate primate affecting bacterial stem cells are evidenced to be disrupted by bigger size AgNps and have the capability to arise demolition of reproductive, digestion activities [10].…”

Section: Journal Of Nutritional Disorders and Therapymentioning

confidence: 99%

Bigger Size Silver Nanoparticles Hamper Mother and Fetus Nutrition after Serial Ingestion of Bolotic Colloidal Nano Silver Stirring Solution through Oral Route: A Revolutionary Research Discovery in Teratogenicity & Toxicity Field

Prakash¹,

Singh²

2017

J Nutr Disorders Ther

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In the field of household products like air conditioner, food protective wrapper silver foil, compressor, fabrics and washing machine manufacture, subscriber or consumer utilized engineered silver nanoparticles of multi bigger size (100 nm size range or more) demand has been extensively increased with increase of negative feedback while using. For the purpose to assess the effect of bigger size silver nano particles on body nutritional status, a serial oral application of silver nanoparticle colloidal stirring cool solution has been made upon pregnant Swiss Albino mice. On serial application (Daily once in morning 9 am continuously from 6 to 19 gestational ages) by repeated gavages during pregnancy pass through portal venous circulation towards liver after gastro intestinal tract absorption hampers mother nutrition by blocking intra hepatic billiary radical and also drastically affect fetal nutrition following same mechanism after crossing blood placental barrier due to gradual accumulation. The present research investigates whether orally induce colloidal silver nanoparticles (AgNps) reach after crossing mother mouse blood placental barrier and induce adverse effects in fetuses from treated group.Pregnant Swiss Albino female mouse from treated group are exposed during the first 13 days of gestation by serial oral bolotic application of bigger size silvernano colloid stirring cool solution at volume of 6, 12, 18, 24 mg/kg b.w. corresponds to individual group when compared with double distilled water vehicle control. Black ash colored bigger silver nanoparticles are identified scattered in maternal and fetal liver, upper GI tissues, placental and umbilical cord tissues of pregnant mother and fetuses after cesarean section and organ removal in gross , histological and TEM view. Particles are found in chronic depositative manner severely blocking portal triad of liver, IHBR and portovenous route. Transmission electron microscopy coupled with energy-dispersive spectroscopy confirmed the size range of particle silvernano from 71 to 1900 nm range.Serial oral ingestion of bigger size AgNps results in increased number of resorbed and malnutriated fetuses associated with reduced mother body and liver (sink & weak) weight and diet. Increased expression of particle accumulation is also detected in the placental cut section of treated groups. The acquired results indicates bigger size nanosilver are able to reach fetus after crossing the pregnant mouse blood placental barrier and portovenous circulation causing hamper of mother and fetus nutrition by obstruction of micro and macro channels. Henceforth as a conclusion, it is wise to take precaution with respect to oral exposure of bigger size silvernanoparticles during pregnancy.

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Silver nanoparticle‐induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues

Cited by 329 publications

References 74 publications

Antibacterial activity of silver nanoparticles: A surface science insight

Antibacterial activity of silver nanoparticles: A surface science insight

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

Bigger Size Silver Nanoparticles Hamper Mother and Fetus Nutrition after Serial Ingestion of Bolotic Colloidal Nano Silver Stirring Solution through Oral Route: A Revolutionary Research Discovery in Teratogenicity & Toxicity Field

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