Silver nanocomposites based on the bacterial fucose-rich polysaccharide secreted by Enterobacter A47 for wound dressing applications: Synthesis, characterization and in vitro bioactivity

Concórdio‐Reis, Patrícia; Pereira, Catarina; Batista, Miguel P.; Sevrin, Chantal; Grandfils, Christian; Marques, Ana C.; Fortunato, Elvira; Gaspar, Frédéric B.; Matias, Ana A.; Freitas, Filomena; Reis, Maria A. M.

doi:10.1016/j.ijbiomac.2020.07.072

Cited by 33 publications

(48 citation statements)

References 63 publications

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“…Thus, cultivation was performed under a fed-batch mode (feeding solution: Medium E* supplemented with 200 g/L of glycerol), with controlled temperature (30.0 ± 0.1 • C), pH (7.0 ± 0.02) and dissolved oxygen concentration (10% of the air saturation). FucoPol was recovered from the cultivation broth after 4 days as previously described [27]. The procedure involved dilution of the broth with deionized water (1:10, v/v) to reduce viscosity, centrifugation (13000×g, 45 min) for cell removal, thermal treatment (70 • C, 1 h) of the cell-free supernatant to promote protein denaturation and, finally, centrifugation (13,000× g, 45 min) for removal of cell fragments and denatured proteins.…”

Section: Fucopol Production Purification and Characterizationmentioning

confidence: 99%

“…The procedure involved dilution of the broth with deionized water (1:10, v/v) to reduce viscosity, centrifugation (13000×g, 45 min) for cell removal, thermal treatment (70 • C, 1 h) of the cell-free supernatant to promote protein denaturation and, finally, centrifugation (13,000× g, 45 min) for removal of cell fragments and denatured proteins. For FucoPol purification, the low molecular weight compounds (salts, glycerol, proteins) were removed by diafiltration in a cross-flow module (Sartocon Slide Holder), using a membrane with a surface area of 100 cm 2 and a 100 kDa nominal molecular weight cut-off (Hydrosart ultrafiltration cassette, Sartorius) [27]. During diafiltration, deionized water was added to the retentate to facilitate the diffusion of low molecular weight solutes across the membrane.…”

Section: Fucopol Production Purification and Characterizationmentioning

confidence: 99%

“…Specifically, the presence of glucuronic acid residues, together with the pyruvyl and succinyl groups, that confer the biopolymer an anionic character, is relevant for its binding ability towards cations and positively charged molecules. FucoPol sample also has a protein content of 10.8%, which was not completely removed during the purification procedures, probably due to their high Mw [27]. The presence of amine, sulfhydryl and carboxyl functional groups in proteins contribute to the overall negative charge of FucoPol [24].…”

Section: Fucopol Characterizationmentioning

confidence: 99%

“…The branches, present at position 3 of the first fucose, are composed of an α-D-4,6-pyruvyl-Galp-(1→4)-β-DGlcAp-(1→3)-α-D-Galp(1→trimer, with two pyruvate caps in the terminal galactose, specifically at position C-4 and C-6 [27,28]. FucoPol was employed for the preparation of a bioactive silver nanocomposite [27] and as a coating agent for iron oxide magnetic nanoparticles (MNP). The use of these FucoPol-MNP was evaluated for human antibody purification [29] and as cell labeling nanoprobes for Magnetic Resonance Imaging [30].…”

Section: Introductionmentioning

confidence: 99%

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Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

2020

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Despite the efforts for minimizing the usage of heavy metals, anthropogenic activities still generate high amounts of wastewater containing these contaminants that cause significant health and environmental problems. Given the drawbacks of the conventional physical and chemical methods currently used, natural biosorbents (microbial cells or their products) arise as promising environmentally friendly alternatives. In this study, the binding efficiency of the polysaccharide secreted by Enterobacter A47, FucoPol, towards lead (Pb2+), cobalt (Co2+), copper (Cu2+) and zinc (Zn2+) cations was demonstrated. FucoPol revealed a higher performance for the biosorption of Pb2+, with a maximum overall metal removal of 93.9 ± 5.3% and a specific metal uptake of 41.1 ± 2.3 mg/gEPS, from a Pb2+ solution with an initial concentration of 10 mg/L, by a 5 g/L FucoPol solution. The overall metal removal decreased considerably (≤31.3 ± 1.6%) for higher Pb2+ concentrations (48 and 100 mg/L) probably due to the saturation of FucoPol’s binding sites. Pb2+ removal was also less efficient (66.0 ± 8.2%) when a higher FucoPol concentration (10 g/L) was tested. Pb2+ removal efficiency of FucoPol was maximized at pH 4.3, however, it was affected by lower pH values (2.5–3.3). Moreover, the FucoPol’s sorption performance was unaffected (overall metal removal: 91.6–93.9%) in the temperature range of 5–40 °C. These findings demonstrate FucoPol’s great potential for utilization as a biodegradable and safe biosorbent for treating waters and wastewaters contaminated with Pb2+.

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Section: Fucopol Production Purification and Characterizationmentioning

confidence: 99%

Section: Fucopol Production Purification and Characterizationmentioning

confidence: 99%

Section: Fucopol Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

2020

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“…Due to their high growth rates of microorganisms, to the possibility of enhancing productivity as well as customizing the biopolymers’ desirable properties by modifying the bioprocess conditions, the bacterial origin polysaccharides are of special interest in the scientific community [ 175 ]. Microbial polysaccharides are mainly exo-polysaccharides that bacteria secrete for their own purposes henceforth they do not provoke a biological response from cells, so they do not biologically interact with human tissues.…”

Section: Electrospun Nanofibers In Wound Healingmentioning

confidence: 99%

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

et al. 2020

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Currently, despite the thoroughgoing scientific research carried out in the area of wound healing management, the treatment of skin injuries, regardless of etiology remains a big provocation for health care professionals. An optimal wound dressing should be nontoxic, non-adherent, non-allergenic, should also maintain a humid medium at the wound interfacing, and be easily removed without trauma. For the development of functional and bioactive dressings, they must meet different conditions such as: The ability to remove excess exudates, to allow gaseous interchange, to behave as a barrier to microbes and to external physical or chemical aggressions, and at the same time to have the capacity of promoting the process of healing by stimulating other intricate processes such as differentiation, cell adhesion, and proliferation. Over the past several years, various types of wound dressings including hydrogels, hydrocolloids, films, foams, sponges, and micro/nanofibers have been formulated, and among them, the electrospun nanofibrous mats received an increased interest from researchers due to the numerous advantages and their intrinsic properties. The drug-embedded nanofibers are the potential candidates for wound dressing application by virtue of: Superior surface area-to volume ratio, enormous porosity (can allow oxy-permeability) or reticular nano-porosity (can inhibit the microorganisms’adhesion), structural similitude to the skin extracellular matrix, and progressive electrospinning methodology, which promotes a prolonged drug release. The reason that we chose to review the formulation of electrospun nanofibers based on polysaccharides as dressings useful in wound healing was based on the ever-growing research in this field, research that highlighted many advantages of the nanofibrillary network, but also a marked versatility in terms of numerous active substances that can be incorporated for rapid and infection-free tissue regeneration. In this review, we have extensively discussed the recent advancements performed on electrospun nanofibers (eNFs) formulation methodology as wound dressings, and we focused as well on the entrapment of different active biomolecules that have been incorporated on polysaccharides-based nanofibers, highlighting those bioagents capable of improving the healing process. In addition, in vivo tests performed to support their increased efficacy were also listed, and the advantages of the polysaccharide nanofiber-based wound dressings compared to the traditional ones were emphasized.

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Biomedical Applications of Green Nanomaterials

Prasher

Sharma

2022

Handbook of Green and Sustainable Nanotechnology

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Silver nanocomposites based on the bacterial fucose-rich polysaccharide secreted by Enterobacter A47 for wound dressing applications: Synthesis, characterization and in vitro bioactivity

Cited by 33 publications

References 63 publications

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

Biomedical Applications of Green Nanomaterials

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