Silver improves collagen structure and stability at demineralized dentin: A dynamic-mechanical and Raman analysis

Osorio, Raquel; Osorio, Estrella; Aguilera, Fátima S.; Medina-Castillo, Antonio L.; Toledano, Manuel

doi:10.1016/j.jdent.2018.10.003

Cited by 15 publications

(9 citation statements)

References 35 publications

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“…90% of the organic matrix is a fibrillar type I, whereas the remaining 10% consists of non-collagenous proteins like phosphoproteins and proteoglycans. The collagen of dentin acts as a scaffold for precipitations of minerals, so it was essential for preservation and maintaining the stability of collagen during the re-mineralization process (5) .…”

Section: Introductionmentioning

confidence: 99%

The Synergetic Effect of Silver Diamine Fluoride with Potassium Iodide and Grape Seed Extract on Dentin Remineralization

2021

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Purpose: This study was conducted to evaluate the synergetic effect of using silver diamine fluoride containing potassium iodide and grape seed extract on dentin microhardness. Materials and Methods: Sixty freshly extracted human deciduous second molars were enrolled in this study. Sixty dentin discs were cut from the crown and were randomly divided into four groups according to the applied treatment after dentin demineralization, (15 samples each). In the first group, samples were received no treatment, only stored in artificial saliva (positive control), in the second group, samples were treated with silver diamine fluoride containing potassium iodide only, in the second group, samples were treated with grape seed extract only (GSE), in the third group, samples were treated with silver diamine fluoride containing potassium iodide (SDF), whereas in the fourth group, samples were treated with combined therapy of grape seed extract followed with silver diamine fluoride containing potassium iodide (GSE+SDF). Microhardness measurements were taken for each dentin disc at baseline, after demineralization, and after treatment. Results: There was a statistically significant increase in dentin microhardness after each treatment as compared to the demineralized dentin microhardness. The mean percent change of dentin microhardness was statistically significant increased in GSE, SDF, and GSE+SDF groups when compared to the control group. Conclusions: Considering the limitations of this study, monotherapy of GSE, SDF solution, and their combination can be recommended to enhance the micro-hardness of demineralized dentin. SDF applications after GSE improved the micro-hardness of demineralized dentin when compared to the monotherapy of each treatment.

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Section: Introductionmentioning

confidence: 99%

The Synergetic Effect of Silver Diamine Fluoride with Potassium Iodide and Grape Seed Extract on Dentin Remineralization

2021

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“…The collagen of dentin as the scaffold for the growing of mineral crystals plays an essential role in dentin remineralization [21]. Moreover, the application of silver nanoparticles can regulate the deposition of collagen and promote further mineralization through the enhancement of collagen crosslinking [22]. The ability to inhibit the bacterial proteolysis can also allow for the degradation of dentin collagen to be avoided [32].…”

Section: Discussionmentioning

confidence: 99%

“…The release of silver ions affects the reactive side chain of bacterial collagenase, which inactivates the catalytic functions of bacteria and preserves dentin collagen [21]. Silver possesses the capability to inhibit the activity of matrix metalloproteinases (MMPs) [22]. It is presumed that Ag + occupies the sites of Ca 2+ in collagen fibrils and protects collagen from the degradation caused by MMPs through competitive inhibition [22].…”

Section: Introductionmentioning

confidence: 99%

“…Silver possesses the capability to inhibit the activity of matrix metalloproteinases (MMPs) [22]. It is presumed that Ag + occupies the sites of Ca 2+ in collagen fibrils and protects collagen from the degradation caused by MMPs through competitive inhibition [22]. AgNPs have shown the ability to regulate collagen deposition and direct proper collagen matrix arrangement in the research of wound healing [23].…”

Section: Introductionmentioning

confidence: 99%

“…AgNPs have shown the ability to regulate collagen deposition and direct proper collagen matrix arrangement in the research of wound healing [23]. Studies have suggested that silver is able to improve the chemical structure and stability of collagen in demineralized dentin [22]. Therefore, we synergized MBG-Ag as a sealer and used Er:YAG laser treatment human dentin specimens, and analyzed the characteristics and antibacterial activity of these specimens after MBG-Ag sealing and Er:YAG laser irradiation in this study.…”

Section: Introductionmentioning

confidence: 99%

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The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

et al. 2021

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The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.

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Unraveling Nanomaterials in Biomimetic Mineralization of Dental Hard Tissue: Focusing on Advantages, Mechanisms, and Prospects

Dai,

Li,

Zhang

2024

Advanced Science

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The demineralization of dental hard tissue imposes considerable health and economic burdens worldwide, but an optimal method that can repair both the chemical composition and complex structures has not been developed. The continuous development of nanotechnology has created new opportunities for the regeneration and repair of dental hard tissue. Increasingly studies have reported that nanomaterials (NMs) can induce and regulate the biomimetic mineralization of dental hard tissue, but few studies have examined how they are involved in the different stages, let alone the relevant mechanisms of action. Besides their nanoscale dimensions and excellent designability, NMs play a corresponding role in the function of the raw materials for mineralization, mineralized microenvironment, mineralization guidance, and the function of mineralized products. This review comprehensively summarizes the advantages of NMs and examines the specific mineralization mechanisms. Design strategies to promote regeneration and repair are summarized according to the application purpose of NMs in the oral cavity, and limitations and development directions in dental hard tissue remineralization are proposed. This review can provide a theoretical basis to understand the interaction between NMs and the remineralization of dental hard tissue, thereby optimizing design strategy, rational development, and clinical application of NMs in the field of remineralization.

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Silver improves collagen structure and stability at demineralized dentin: A dynamic-mechanical and Raman analysis

Cited by 15 publications

References 35 publications

The Synergetic Effect of Silver Diamine Fluoride with Potassium Iodide and Grape Seed Extract on Dentin Remineralization

The Synergetic Effect of Silver Diamine Fluoride with Potassium Iodide and Grape Seed Extract on Dentin Remineralization

The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

Unraveling Nanomaterials in Biomimetic Mineralization of Dental Hard Tissue: Focusing on Advantages, Mechanisms, and Prospects

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