Purpose: This study was conducted to evaluate the synergetic effect of using silver diamine fluoride containing potassium iodide and grape seed extract on dentin microhardness. Materials and Methods: Sixty freshly extracted human deciduous second molars were enrolled in this study. Sixty dentin discs were cut from the crown and were randomly divided into four groups according to the applied treatment after dentin demineralization, (15 samples each). In the first group, samples were received no treatment, only stored in artificial saliva (positive control), in the second group, samples were treated with silver diamine fluoride containing potassium iodide only, in the second group, samples were treated with grape seed extract only (GSE), in the third group, samples were treated with silver diamine fluoride containing potassium iodide (SDF), whereas in the fourth group, samples were treated with combined therapy of grape seed extract followed with silver diamine fluoride containing potassium iodide (GSE+SDF). Microhardness measurements were taken for each dentin disc at baseline, after demineralization, and after treatment. Results: There was a statistically significant increase in dentin microhardness after each treatment as compared to the demineralized dentin microhardness. The mean percent change of dentin microhardness was statistically significant increased in GSE, SDF, and GSE+SDF groups when compared to the control group. Conclusions: Considering the limitations of this study, monotherapy of GSE, SDF solution, and their combination can be recommended to enhance the micro-hardness of demineralized dentin. SDF applications after GSE improved the micro-hardness of demineralized dentin when compared to the monotherapy of each treatment.
Introduction: There is an interest in developing materials with bioactive potential that could block exposed dentinal tubules. This study compared the effects of eggshell and seashell nanoparticles individually or combined with ND:YAG laser on dentinal tubules occlusion and remineralization. Methods: Fifty radicular dentin discs were prepared from freshly extracted human premolars. The smear layer created by cutting was removed using 37% phosphoric acid gel for 15 sec. The discs were divided into five groups according to the applied treatment(A) (n = 10 each): (A1) control, (A2); Nano eggshells, (A3); Nano seashells, (A4); Nano eggshells + Nd: YAG Laser, and (A5); Nano sea shell + Nd: YAG Laser. Each specimen was evaluated for tubular patency and mineral contents before and after each therapy using ESEM-EDXA energy dispersive spectroscopy for the assessment of tubule occlusion and remineralization. Results: ESEM results revealed a statistically significant decrease in the mean percent changes of the dentinal tubules number after the treatment of the experimental groups compared to the control. The greatest percent decrease was recorded in the seashell NPs + Nd: YAG laser, followed by the eggshell NPs + Nd: YAG laser, then Eggshell NPs only and then Seashell NPs only, while the lowest percentage decrease was recorded in the control group. EDXA revealed that the greatest percentage increase in Ca wt% was recorded in the Eggshell + Nd:YAG laser group, followed by Eggshell only, then Seashell only and then Seashell NPs + Nd: YAG laser, while the lowest percent increase was recorded in the control group. The post hoc test revealed no significant difference between the experimental groups. Conclusions: Both eggshell and seashell nanoparticles are effective in the occlusion and remineralization of dentinal tubules. The combined treatments with Nd: YAG laser had no benefits when compared to the effect of treatments alone.
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