Silver Impregnation of Ciliated Protozoa by the Chatton-Lwoff Technic

Corliss, John O.

doi:10.3109/10520295309105108

Cited by 307 publications

(84 citation statements)

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“…These morphological characteristics are the same as M. avidus. Although various silver impregnation methods have been used to identify scuticociliatida (Corliss 1953, Foissner 1991, there is difficulty in identification of various marine scuticociliates. Song & Wilbert (2000) re-identified various marine scuticociliates and reported that 14 forms had been misidentified or were junior synonyms.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)

Song¹,

Kitamura²,

Oh³

et al. 2009

Dis. Aquat. Org.

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The scuticociliates Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum were cloned and identified using morphological characteristics and the small subunit ribosomal RNA gene (SSU rRNA). M. avidus strains YS1, WS1, YK1 and JJ3 from southern coastal areas and Jeju Island in Korea were pathogenic to olive flounder Paralichthys olivaceus (80 to 100% mortality in 8 to 10 g fish) when inoculated intraperitoneally (i.p.) with 1.0 to 1.4 × 10 6 ciliates fish -1. Mortality was lower (10 to 45%) when the inoculum was 1.0 to 1.4 × 10 4 ciliates fish -1 in the i.p.-injected group. The M. avidus strains of YS1, WS1, YK1 and JJ3 caused 60 to 100% mortality by immersion infection with 3.2 to 4.2 × 10 3 ml -1 in 8 to 10 g fish and 3.0 to 4.0 × 10 3 ml -1 in 30 to 40 g fish. M. avidus strain Mie0301 from the Mie prefecture in Japan caused 70% mortality by immersion infection with 4.4 × 10 3 ml -1 in 30 to 40 g fish. The predominant sign was severe abdominal distension in i.p.-injected fish, and extensive ulcer lesions in the skeletal muscle in immersion-infected fish. Numerous ciliates were observed in the ascetic fluid, ulcers, haemorrhagic lesions, gills and brain of infected fish. However, P. persalinus (strain SCL-A), P. hargisi (strain SCL-B) and U. marinum (strain JK3) showed less than 30% mortality from both i.p. and immersion challenges, with no ciliate invasion in the skin, gills or brain. M. avidus-infected fish showed many ciliates in gills, fins, skin muscle, brain and intestine accompanied by necrosis and haemorrhages. However, no histological changes were observed in P. persalinus-, P. hargisi-or U. marinum-infected fish. KEY WORDS: Pathogenicity · Scuticociliatida · Miamiensis avidus · Philasterides dicentrarchi · Pseudocohnilembus persalinus · Pseudocohnilembus hargisi · Uronema marinum · Olive flounder · Paralichthys olivaceus Resale or republication not permitted without written consent of the publisherDis Aquat Org 83: [133][134][135][136][137][138][139][140][141][142][143] 2009 1964) and silver pomfret Pampus argenteus (Azad et al. 2007).Commercially significant occurrences of scuticociliatosis in olive flounder in Korea were first noted in 1990 (Chun 2000). The ciliates occurred in the gills, skin, heart, brain, muscles and visceral organs including the intestine. Scuticociliatosis is highly histophagous and destroys infected tissues. The causative agents of scuticociliatosis in the olive flounder in Korea with the same clinical signs mentioned above have been isolated and identified as Uronema marinum (Jee et al. 2001), Pseudocohnilembus persalinus (Kim et al. 2004b), Philasterides dicentrarchi (Kim et al. 2004a) and Miamiensis avidus (Jung et al. 2005). In addition, a very similar disease with high mortality caused by an unidentified scuticociliate in olive flounder juveniles was also reported in Japan (Yoshinaga & Nakazoe 1993). However, it is not clear if all the species of scuticociliates cause mortalities with s...

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)

Song¹,

Kitamura²,

Oh³

et al. 2009

Dis. Aquat. Org.

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“…For permanent preparations the smeared slides are semi air-dried, fixed in Schaudinn's fluid and stained in Heidenhain's iron alum haematoxylin for general staining. Silver impregnation techniques after Corliss (1953); Klein (1958) were employed to study the infra ciliature and kineties of ciliates. The prevalence was calculated as number of infested oysters divided by number of observed oysters multiplied by hundred.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of a protozoan parasite Cristigera sp. (Ciliophora:Ciliatea) from edible oysters (Mollusca:Bivalvia) of Sundarbans, West Bengal, India

Biswas

Bandyopadhyay

2013

J Parasit Dis

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The coastal region of West Bengal is bestowed with a wide range of natural forest and aquatic resources. The estuarine complex is a culmination of the interaction of land, sea and freshwater. It offers diverse specialised habitats such as mangroves, non vegetated mudflats, inter tidal zones and reclaimed areas. Such areas are home to a number of terrestrial, freshwater and marine communities. Edible oyster resources in these regions are Crassostrea gryphoides and Saccostrea cucullata, which are the keystone species found in the intertidal zone and can tolerate huge variation of salinity. These are used as food by local people and marketed to earn cash. The population of this species has been declined due to parasitic infection and pollution. There are many protozoan parasites which infect these oysters causing diseases. During survey period, a protozoan parasite of the genus Cristigera have been observed only from the edible oyster Crassostrea gryphoides collected from Kaikhali and Frasergunj among three selected sites namely Kaikhali, Frasergunj and Digha of West Bengal mainly during monsoon and post-monsoon season. Considering such rare and specific prevalence of Cristigera sp, it may be considered as potential bioindicator.

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“…(1984). The silverline system was studied by the dry and wet silver nitrate methods of Foissner (1976) and Corliss (1953a), respectively. All measurements were carried out by an ocular micrometer.…”

Section: Methodsmentioning

confidence: 99%

Taxonomy and ecology of some ciliates (Protozoa, Ciliophora) of the saprobic system. III. Revision of the genera Colpidium and Dexiostoma, and establishment of a new genus, Paracolpidium nov. gen.

Ganner

Foissner

1989

Hydrobiologia

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The genera Colpidium Stein, 1860 and Dexiostoma Jankowski, 1967 are revised. Our monographic treatment is based on a morphologic and biometric reinvestigation of the main species of these genera and on an evaluation of data from the literature. Colpidium comprises four species, namely C. colpoda (Losana, 1829), C. singulare Vuxanovici, 1961, C. acuminatum Vuxanovici, 1962, and C. kleini Foissner, 1969. The monotypic genus Dexiostoma contains D. campyla (Stokes, 1886) and is distinguished from Colpidium by a different oral infraciliature and the position and shape of the preoral suture. A new genus, Paracolpidium nov. gen., is suggested for Colpidium truncatum Stokes, 1885 because its oral infraciliature deviates from both Colpidium and Dexiostoma. Contrary to Colpidium and Dexiostoma, the silverline system of Paracolpidium truncatum lacks secondary meridians but shows short projections to the left of the primary meridians indicating a phylogenetic relationship to Tetrahymena.

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Silver Impregnation of Ciliated Protozoa by the Chatton-Lwoff Technic

Cited by 307 publications

References 0 publications

Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)

Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)

Prevalence of a protozoan parasite Cristigera sp. (Ciliophora:Ciliatea) from edible oysters (Mollusca:Bivalvia) of Sundarbans, West Bengal, India

Taxonomy and ecology of some ciliates (Protozoa, Ciliophora) of the saprobic system. III. Revision of the genera Colpidium and Dexiostoma, and establishment of a new genus, Paracolpidium nov. gen.

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