Silver Colloids Impregnating or Coating Bacteria

Efrima, Shlomo; Bronk, Burt V.

doi:10.1021/jp9813903

Cited by 167 publications

(155 citation statements)

References 18 publications

(31 reference statements)

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“…However, there are several limitations with these methods; bare nanoparticles do not readily penetrate cellular membranes, and even once inside the cell, they cannot move freely. A promising alternative has been to grow gold nanoparticles within bacteria [8][9][10][11] or human cells by infusion of a low-density chloroaurate solution 12,13 , albeit without the means to control location.…”

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confidence: 99%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

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confidence: 99%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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“…Recently, a type of SERS-active substrate with uniformly large and highly reproducible Raman-enhancing power has been developed by growing Ag nanoparticles on arrays of anodic aluminum oxide (AAO) nanochannels to take advantage of the sub-10-nm inter-particle gaps, which act as 'hot junctions' for creating the electromagnetic enhancement 4 . The high sensitivity and reproducibility of such a substrate-hereafter referred to as Ag/AAO-SERS substrate-facilitated the use of SERS for chemical/biological sensing applications [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] . SERS of various types of bacteria including Gram-positive, Gram-negative and mycobacteria have been acquired and the response of bacteria to antibiotics has been examined 21 .…”

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confidence: 99%

Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

et al. 2011

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Detecting bacteria in clinical samples without using time-consuming culture processes would allow rapid diagnoses. such a culture-free detection method requires the capture and analysis of bacteria from a body fluid, which are usually of complicated composition. Here we show that coating Ag-nanoparticle arrays with vancomycin (Van) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (sERs), leading to a ~1,000-fold increase in bacteria capture, without introducing significant spectral interference. Bacteria from human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, a Van-coated substrate provides distinctly different sERs spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance tests. our results represent a critical step towards the creation of sERs-based multifunctional biochips for rapid culture-and label-free detection and drug-resistant testing of microorganisms in clinical samples.

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“…The NPs can be either coated on the outside of the bacterial cell wall or directed to the interior of the bacterial cells. Whereas the first preparation results in spectral information mainly derived from cell wall components, the second one contains additional cytoplasmic information [18,19]. Figure 1 shows the SERS signal acquisition process from a microbiologic sample, when the silver coverage of the bacteria (in blue) is successful.…”

Section: Sers Effectmentioning

confidence: 99%

The Intricate Nature of SERS: Real‐Life Applications and Challenges

Dina¹,

Colniță²

2017

Raman Spectroscopy and Applications

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Testing for the presence of microorganisms in biological samples in order to diagnose infections is very common at all levels of health care. There is a growing need to ensure appropriate diagnosis by also minimizing the analysis time, both being very important concerns related to the risk of developing an antimicrobial resistance. Moreover, there are important medical and financial implications associated with infections. In this chapter, we will discuss the latest ultrasensitive and selective, but simple, rapid and inexpensive bacteria detection and identification methods by using receptor-free and innovative immobilization principles of the biomass. Raman spectroscopy, which combines the selectivity of the method with the sensitivity of the surface-enhanced Raman scattering (SERS) effect, is used in correlation with chemometric techniques in order to develop biosensors for pathogenic microorganisms.

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Silver Colloids Impregnating or Coating Bacteria

Cited by 167 publications

References 18 publications

Laser-targeted photofabrication of gold nanoparticles inside cells

Laser-targeted photofabrication of gold nanoparticles inside cells

Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

The Intricate Nature of SERS: Real‐Life Applications and Challenges

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