Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

Liu, Ting Yu; Tsai, Kun-Tong; Wang, Huai Hsien; Chen, Yu; Chao, Yuan Chun; Chang, Hsuan; Lin, Chi; Wang, Juen Kai; Wang, Yuh‐Lin

doi:10.1038/ncomms1546

Cited by 237 publications

(127 citation statements)

References 44 publications

(50 reference statements)

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“…37 Therefore, vancomycinmodified nanostructures can interact strongly with a broad range of Gram-positive and Gram-negative bacteria and thus can be used as affinity probes to selectively trap these organisms. 21,53 On the basis of the knowledge in the previous studies combined with our experimental results, the plausible mechanism of the enhanced antibacterial activity of Van/Fe 3 O 4 @ SiO 2 @Ag microflowers is proposed in Figure 7A. The Van/ Fe 3 O 4 @SiO 2 @Ag microflower can interact with the cell wall of the bacteria and adhere onto the bacterial surface …”

Section: @Ag Microflowersmentioning

confidence: 99%

Vancomycin-modified Fe3O4@SiO2@Ag microflowers as effective antimicrobial agents

Wang

Zhang²,

Zhou³

et al. 2017

IJN

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Nanomaterials combined with antibiotics exhibit synergistic effects and have gained increasing interest as promising antimicrobial agents. In this study, vancomycin-modified magnetic-based silver microflowers (Van/Fe 3 O 4 @SiO 2 @Ag microflowers) were rationally designed and prepared to achieve strong bactericidal ability, a wide antimicrobial spectrum, and good recyclability. High-performance Fe 3 O 4 @SiO 2 @Ag microflowers served as a multifunction-supporting matrix and exhibited sufficient magnetic response property due to their 200 nm Fe 3 O 4 core. The microflowers also possessed a highly branched flower-like Ag shell that provided a large surface area for effective Ag ion release and bacterial contact. The modified-vancomycin layer was effectively bound to the cell wall of bacteria to increase the permeability of the cell membrane and facilitate the entry of the Ag ions into the bacterium, resulting in cell death. As such, the fabricated Van/Fe 3 O 4 @SiO 2 @Ag microflowers were predicted to be an effective and environment-friendly antibacterial agent. This hypothesis was verified through sterilization of Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus , with minimum inhibitory concentrations of 10 and 20 μg mL −1 , respectively. The microflowers also showed enhanced effect compared with bare Fe 3 O 4 @SiO 2 @Ag microflowers and free-form vancomycin, confirming the synergistic effects of the combination of the two components. Moreover, the antimicrobial effect was maintained at more than 90% after five cycling assays, indicating the high stability of the product. These findings reveal that Van/Fe 3 O 4 @SiO 2 @Ag microflowers exhibit promising applications in the antibacterial fields.

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Section: @Ag Microflowersmentioning

confidence: 99%

Vancomycin-modified Fe3O4@SiO2@Ag microflowers as effective antimicrobial agents

Wang

Zhang²,

Zhou³

et al. 2017

IJN

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“…In particular, surface‐enhanced Raman scattering spectroscopy is able to enhance the signal by 11 orders of magnitude, which is sufficient for single‐molecule detection 14. Therefore, micro‐Raman spectroscopy has been utilized to monitor bacterial development, probe cellular stress response, analyze functions of microbial communities, and detect clinically relevant bacteria in laboratory settings 11, 12, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24. We also utilized Raman microspectroscopy to probe bacterial metabolism and interactions9, 25 and isolate single cells 26.…”

Section: Introductionmentioning

confidence: 99%

Culture‐Free Detection of Crop Pathogens at the Single‐Cell Level by Micro‐Raman Spectroscopy

Gan

Wang

et al. 2017

Advanced Science

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The rapid and sensitive identification of invasive plant pathogens has important applications in biotechnology, plant quarantine, and food security. Current methods are far too time‐consuming and need a pre‐enrichment period ranging from hours to days. Here, a micro‐Raman spectroscopy‐based bioassay for culture‐free pathogen quarantine inspection at the single cell level within 40 min is presented. The application of this approach can readily and specifically detect plant pathogens Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi that are closely related pathogenically. Furthermore, the single‐bacterium detection was able to discriminate them from a reference Raman spectral library including multiple quarantine‐relevant pathogens with broad host ranges and an array of pathogenic variants. To show the usefulness of this assay, Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi are detected at single‐bacterium level in plant tissue lesions without pre‐enrichment. The results are confirmed by the plate‐counting method and a genetic molecular approach, which display comparable recognition ratios to the Raman spectroscopy‐based bioassay. The results represent a critical step toward the use of micro‐Raman spectroscopy in rapid and culture‐free discrimination of quarantine relevant plant pathogens.

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“…Such a study is difficult but immensely useful because the hybrid (metal-semiconductor) nanostructures can be used for a wide range of applications such as SERS, [16][17][18] photonics, 4,5 renewable energy 3 and biomedicine. [19][20][21] This study addresses whether the newly observed optical effects, LMR and SWs in an NW system, can positively affect the plasmonic E field for photonic applications.…”

Section: Introductionmentioning

confidence: 99%

Plasmon management in index engineered 2.5D hybrid nanostructures for surface-enhanced Raman scattering

Huang¹,

Chen

et al. 2014

NPG Asia Mater

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Leaky mode resonance (LMR) and optical standing waves (SWs) generated in suitably illuminated vertically aligned supported nanowires (NWs) were demonstrated to enhance the plasmonic electric (E) field behavior of silver nanoparticles (AgNPs) dispersed on the wires. The combination of LMR and SW can significantly enhance the local plasmonic E field around highdensity AgNPs. The results of the finite difference time domain (FDTD) calculations were experimentally verified by comparing the surface-enhanced Raman scattering (SERS) sensitivity, which is directly dependent on the E field, in AgNP-coated silicon, germanium and silver NWs. As LMR and SW are functions of the substrate optical index (n, k), an engineering of the indices predicted a (3.88, 0) theoretical value to maximize the plasmonic E-field on the hybrid scaffold at a given AgNP density. These SERS substrates were utilized for the detection of marine toxins, L-BMAA (2-amino-3-(methylamino) propionic acid hydrochloride) and Malachite green, which are used extensively in seafood. AgNP-decorated silicon NWs, whose index (3.88, 0.02) lies close to the theoretically predicted value, exhibit at least pico-molar sensitivity toward those marine toxins. A plasmon management strategy is developed that could assist in lowering the detection level of environmental toxins by SERS. NPG Asia Materials (2014) 6, e123; doi:10.1038/am.2014.67; published online 12 September 2014 INTRODUCTIONThe involvement of plasmonics 1 in applications such as biosensing 2 through surface-enhanced Raman scattering (SERS), energy, 3 photodetectors 4 and lasers, 5 among others, is extensive because surface plasmons can concentrate and manipulate light energy on scales lower than their diffraction limit. 6 The common goal has generally been to be able to intensify and control the plasmonic electric (E) fields in preferred locations within the material. For example, the so-called 'hot-spots' in SERS, for molecular sensing, are reportedly the regions of extraordinarily intense E field at the junctions of critically close gold (Au) or silver (Ag) nanoparticles (NPs). The interparticle spacing 7 and fractal-like aggregations 8 within the metal NPs were observed to be more critical for the E enhancement than the basic material properties or the size of the NPs.The recent observation of enhanced optical absorption in suitably illuminated semiconductor nanowires (NWs) via leaky mode resonances (LMRs) 9 opens up the possibility of using LMR for the E-field enhancement of metal NPs. In addition, researchers have observed optical standing waves (SWs) along the length of the NWs when studying optically illuminated vertically aligned NW structures grown on a substrate by finite difference time domain (FDTD) calculations. However, the effect of the SWs or the LMR in modulating the total effective E field has been ignored or addressed inadequately. The fact that Ag-or AuNP-dispersed vertically aligned 1D NW/nanorods 10

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Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

Cited by 237 publications

References 44 publications

Vancomycin-modified Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@Ag microflowers as effective antimicrobial agents

Vancomycin-modified Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@Ag microflowers as effective antimicrobial agents

Culture‐Free Detection of Crop Pathogens at the Single‐Cell Level by Micro‐Raman Spectroscopy

Plasmon management in index engineered 2.5D hybrid nanostructures for surface-enhanced Raman scattering

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