Silk Protein Sericin: Structure, Secretion, Composition and Antimicrobial Potential

Chanu, Shamjetshabam Babeeta; Devi, Sanjenbam Kunjeshwori; Singh, L. Robindro

doi:10.2174/9789811432736119010011

Cited by 3 publications

(4 citation statements)

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“…In the present work, the amino acid composition indicated a high degree of sericin purity, with little or no contaminants. Sericin consisted mainly of polar amino acids (44.05%), while nonpolar amino acids were present by only 20.05%, which may differ according to the cocoon type [ 20 ]. The polar amino acids in the extracted sericin were serine, threonine, proline, isoleucine and cysteine.…”

Section: Resultsmentioning

confidence: 99%

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Diab

Tayea

Elwakil

et al. 2022

IJMS

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Infected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising antibacterial activity, namely sericin/propolis/Amoxicillin nanoparticles, was assessed as a potent treatment of infected wounds in normal and diabetic rats. Skin wound healing efficiency was assessed through wound healing scorings, bacterial load assessment and histological examinations. It was revealed that upon using sericin/propolis/Amoxicillin nanoparticles, complete wound healing was successfully achieved after 10 and 15 days postinjury for nondiabetic and diabetic rats, respectively. However, the bacterial load in the induced infected wounds was extremely low (0–10 CFU/mL) after 15 days post-treatment. The histological studies revealed that the dermis was more organized with new matrix deposition, and mature collagen fibers were observed among the treated animal groups. The present study is the first preclinical study which reported the importance of silk sericin in the form of nano-sericin/propolis loaded with Amoxicillin as an effective treatment against bacterial wound infections.

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Section: Resultsmentioning

confidence: 99%

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Diab

Tayea

Elwakil

et al. 2022

IJMS

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“…Therefore, silk proteins can be used in numerous applications, such as in the treatment of fabrics for medicinal uses and the preparation of wound healing gels. A particular coating of sericin on polyamide or polyester had been used as media for air filters, which were found to assist in sanitizing the contaminated air [ 9 , 52 , 53 ]. In the literature, there are several studies about the investigation of the antibacterial and antifungal activities of silk proteins, but reports on their antiviral properties are relatively rare.…”

Section: Discussionmentioning

confidence: 99%

“…Sericin is easily hydrolyzed although it is considered an insoluble protein in cold water. Sericin is an useful protein owing to its unique features such as oxidation resistance, antibacterial potential, UV resistance, capacity to absorb and release moisture easily, and tyrosine kinase inhibitory activity [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Sericin is also a glycoprotein that possesses many biological characteristics, including antioxidation, immunomodulation, and inhibition of elastase and tyrosinase activities.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

El‐Fakharany

Abu‐Serie

Habashy

et al. 2022

JFB

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Bacterial polymeric silk is produced by Bacillus sp. strain NE and is composed of two proteins, called fibroin and sericin, with several biomedical and biotechnological applications. In the current study and for the first time, the whole bacterial silk proteins were found capable of exerting antiviral effects against herpes simplex virus type-1 (HSV-1), adenovirus type 7 (AD7), and hepatitis C virus (HCV). The direct interaction between bacterial silk-like proteins and both HSV-1 and AD7 showed potent inhibitory activity against viral entry with IC50 values determined to be 4.1 and 46.4 μg/mL of protein, respectively. The adsorption inhibitory activity of the bacterial silk proteins showed a blocking activity against HSV-1 and AD7 with IC50 values determined to be 12.5 and 222.4 ± 1.0 μg/mL, respectively. However, the bacterial silk proteins exhibited an inhibitory effect on HSV-1 and AD7 replication inside infected cells with IC50 values of 9.8 and 109.3 μg/mL, respectively. All these results were confirmed by the ability of the bacterial silk proteins to inhibit viral polymerases of HSV-1 and AD7 with IC50 values of 164.1 and 11.8 μg/mL, respectively. Similarly, the inhibitory effect on HCV replication in peripheral blood monocytes (PBMCs) was determined to be 66.2% at concentrations of 100 μg/mL of the bacterial silk proteins. This antiviral activity against HCV was confirmed by the ability of the bacterial silk proteins to reduce the ROS generation inside the infected cells to be 50.6% instead of 87.9% inside untreated cells. The unique characteristics of the bacterial silk proteins such as production in large quantities via large-scale biofermenters, low costs of production, and sustainability of bacterial source offer insight into its use as a promising agent in fighting viral infection and combating viral outbreaks.

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“…Furthermore, 2% of sericin treatments showed lower browning activity, color changes, and browning enzymatic activities. Sericin is a natural protein with one-third of all amino acids that can be hydrolyzed to small peptides and they have shown antifungal and antibacterial activities (Chanu, Devi, & Singh, 2019). Jongsri, Rojsitthisak, Wangsomboondee, and Seraypheap (2017) investigated chitosan coating combined with spermidine (0.1 ppm) for 'Nam Dok Mai' mango at 25 ± 2 °C.…”

Section: Antifungal Polypeptides On Mangomentioning

confidence: 99%

Lacto-fermented polypeptides integrated with edible coatings for mango (Mangifera indica L.) bio-preservation

et al. 2022

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Silk Protein Sericin: Structure, Secretion, Composition and Antimicrobial Potential

Cited by 3 publications

References 0 publications

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

Lacto-fermented polypeptides integrated with edible coatings for mango (Mangifera indica L.) bio-preservation

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