Silk peptide treatment can improve the exercise performance of mice

Kim, Jisu; Hwang, Hyeon‐Jong; Park, Jonghoon; Yun, Hea Yeon; Suh, Heajung; Lim, Kiwon

doi:10.1186/1550-2783-11-35

Cited by 16 publications

(16 citation statements)

References 22 publications

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“…At the end of the experimental protocol, the resting metabolic rate was measured by indirect calorimetry, using an open-circuit apparatus as previously described. [19][20][21][22] The flow rate was kept constant at 1.2 L/min and measured for 24 h. Mice had free access to food and water. O 2 uptake and CO 2 production were analyzed using a mass spectrometer (RL-600; Alco System, Chiba, Japan) and switching system (ANI6-A-S; Alco System).…”

Section: Respiratory Gas Analysismentioning

confidence: 99%

High-Fat Diet Increases Fat Oxidation and Promotes Skeletal Muscle Fatty Acid Transporter Expression in Exercise-Trained Mice

Yun

Lee

Jeong

2020

Journal of Medicinal Food

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The aim of this study was to investigate the effect of a high-fat diet (HFD) on energy substrate utilization during long-term endurance exercise in mice. Male ICR mice (n = 32; 6 weeks old) were divided into two groups: low-fat diet (LFD, n = 16) and HFD (n = 16) and acclimatized to LFD or HFD feeding over 12 weeks. After 12 weeks, the two dietary groups were each divided into two groups with or without exercise (EX): LF-CON, LF-EX, HF-CON, and HF-EX groups. The exercise groups were trained to run on a treadmill for 12 weeks. At the end of the experimental protocol, energy metabolism in the whole body was measured at rest for 24 h and during exercise for 1 h using respiratory gas analysis. Furthermore, molecules involved in skeletal muscle fat metabolism were analyzed. Substrate utilization for energy metabolism in the whole body indicated that fat utilization was high in HFD intake. Notably, when HFD intake and exercise were combined, fat utilization was markedly increased during endurance exercise. In contrast, exercise showed no effect when combined with LFD intake. The gene expressions of Fat/Cd36, Fatp1, Fabp-pm, and Cpt1 were upregulated by HFD intake, with Fat/Cd36 and Cpt1 considerably elevated during long-term endurance exercise. In contrast, exercise showed no effect when combined with LFD intake. These results suggest that HFD intake effectively increased fat utilization as an energy substrate during long-term endurance exercise.KEYWORDS: endurance exercise fat oxidation fatty acid transporter high-fat diet skeletal muscle *These authors contributed equally to this work.

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Section: Respiratory Gas Analysismentioning

confidence: 99%

High-Fat Diet Increases Fat Oxidation and Promotes Skeletal Muscle Fatty Acid Transporter Expression in Exercise-Trained Mice

Yun

Lee

Jeong

2020

Journal of Medicinal Food

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“…e SF hydrolysates that are prepared by performing acid hydrolysis or enzymatic hydrolysis consist of a mixture of AAs and oligopeptides that are known to exhibit beneficial effects on animal models. e bioactive peptides derived from SF are reported to reduce blood cholesterol level, increase antigenotoxicity, inhibit angiotensin-converting enzyme activity, enhance insulin sensitivity and glucose metabolism, improve alcohol metabolism, and stimulate osteoblastic differentiation [23,[25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Application of Food-Grade Proteolytic Enzyme for the Hydrolysis of Regenerated Silk Fibroin fromBombyx mori

Joung

Park

You

et al. 2018

Journal of Chemistry

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In vitro biodegradation of Bombyx mori silk fibroin (SF) was studied using food-grade proteolytic enzymes to replace acid hydrolysis. Based on the residual protein quantity and yield of amino acids (AAs) after enzymatic hydrolysis, we evaluated the proteolytic enzyme process of SF. FoodPro and Alcalase that are classified as alkaline proteases are selected as two of the best candidate enzymes for hydrolysis of SF. e activity of these enzymes exhibits a broad range of pH (6.5 to 9.0) and temperature (50°C to 65°C). e single enzyme treatment of SF using FoodPro exhibited a hydrolytic efficiency of 20-25%, and >2 g/L AAs were released after reaction for 3 h. A 2-stage enzymatic treatment using a combination of FoodPro and Flavourzyme in a sequence for a reaction time of 6 h was developed to enhance the efficiency of the proteolytic process. e yield of AAs and residual protein quantity in the enzymatic hydrolysates obtained from FoodPro-treated regenerated SF in the 1st step was 2,040 ± 23.7 mg/L and 70.6%, respectively. e yield of AAs was > two-fold (4,519 ± 42.1 mg/L), whereas the residual protein quantity decreased to 55.1% after the Flavourzyme treatment (2nd step) compared to those of the single FoodPro treatment. In the mixed treatment by simultaneously using FoodPro and Flavourzyme, approximately 45% of SF was degraded and 4.5 g/L of AAs were released within 3 h of reaction time. e regenerated SF and its enzymatic hydrolysates were characterized by performing UV-visible spectra, gel electrophoresis, and size-exclusion chromatography analyses. In the 2-stage treatment using FoodPro initially and subsequently Flavourzyme, the aggregates and high molecular weight proteins of SF were dissociated and degraded into the low molecular weight proteins/peptides (10-15 kDa and 27 kDa). SF hydrolysates as functional food might be enzymatically produced using the commercial food-grade proteolytic enzymes.

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“…Recently, we reported that SP (800 mg/kg) treatment with endurance training (approximately 70-75% maximal oxygen uptake) improved fat oxidation at rest and during exercise considerably more than the control treatment with endurance training 13 , 14 . However, it is still unclear whether SP administration would only affect whole-body fat utilization and alter gene expression related to fat metabolism in muscle tissues.…”

Section: Introductionmentioning

confidence: 95%

Effects of Silk Peptides Administration on Fat Utilization Over a Whole Day in Mice

Kim

Park

Lee

et al. 2016

Journal of Exercise Nutrition &Amp; Biochemistry

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[Purpose]The aim of the present study was to investigate the effects of treatment with silk peptides (SP) on resting energy expenditure over a 24-h period and clarify the molecular mechanism underlying its enhancement of fat utilization in mouse skeletal muscles.[Methods]Sixteen male ICR mice (6-weekold) were divided into two groups and treated with distilled water and SP (CON and SP treatment groups, respectively). SP were dissolved in distilled water and administered to the SP group at 800 mg/kg while the CON group was administered distilled water orally daily for 2 weeks. Resting metabolic rate (RMR) was measured before and after SP ingestion for 2 weeks using an open circuit calorimetry system. After the treatments, we collected blood and skeletal muscle samples from the mice.[Results]Final body weight and body weight gain showed no significant difference in the CON and SP groups. Conversely, food intake was significantly lower in the SP group than it was in the CON group. The comparison of the respiratory exchange rate (RER) at various time points after the 2-week treatment revealed that it was significantly lower in the SP group than it was in the CON group at 9, 15, 16, and 18-22 h (Figure 3 B). The sum of the RER over 24-h was lower in the SP group than it was in the CON group, but the difference was not statistically significant. The protein levels of fatty acid translocase (FAT)/ cluster of differentiation 36 (CD36) were approximately 12% higher in the SP group than they were in the CON group but that of carnitine palmitoyltransferase I (CPTІ) was not significantly different between the two groups.[Conclusion]These results suggest that treatment with SP 800 mg/kg for 2 weeks may promote fat utilization during physical activity, but not the entire day. In addition, SP treatment effectively enhanced FAT/CD36 protein level in skeletal muscle. In future investigations, it would be necessary to elucidate the effects of long-term SP intake on the resting metabolism of both animals and humans.

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Silk peptide treatment can improve the exercise performance of mice

Cited by 16 publications

References 22 publications

High-Fat Diet Increases Fat Oxidation and Promotes Skeletal Muscle Fatty Acid Transporter Expression in Exercise-Trained Mice

High-Fat Diet Increases Fat Oxidation and Promotes Skeletal Muscle Fatty Acid Transporter Expression in Exercise-Trained Mice

Application of Food-Grade Proteolytic Enzyme for the Hydrolysis of Regenerated Silk Fibroin fromBombyx mori

Effects of Silk Peptides Administration on Fat Utilization Over a Whole Day in Mice

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