Silk fibroin/polycaprolactone nanofibrous membranes loaded with natural Manuka honey for potential wound healing

Abstract

Antibacterial wound dressing, an effective barrier between the wound and the foreign environment, has the ability to inhibit and even kill the bacteria around the wound so that it can accelerate the wound healing. However, the loaded antibacterial agents of antibiotics and metal ions on wound dressings caused the new problems of drug resistance and toxicity. To develop a novel wound dressing with the natural antibacterial property agent, here, natural Manuka honey (MH), silk fibroin (SF), and polycaprolactone (PCL) were mixed to prepare nanofibrous membranes by electrospinning. The SEM observation showed the good microscopic morphology of SF/PCL/MH nanofibrous membranes that the fibers were even and smooth. With the addition of MH, the membranes gained the accepted stress and the excellent flexibility to adapt to wound contour. In addition, their hydrophilic property was beneficial to cell adhesion and proliferation. Most importantly, SF/PCL/MH nanofibrous membranes exhibited excellent sustained release effect and antibacterial properties.The antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans reached 99.88%, 99.70%, and 98.95%, respectively when 75% natural antimicrobial agent MH was loaded. Moreover, SF/PCL/MH membranes showed good cytocompatibility. These results indicated the fabricated SF/PCL/MH nanofibrous membranes are a kind of novel dressing with good antimicrobial property.

Conductive and antibacterial scaffold with rapid crimping property for application prospect in repair of peripheral nerve injury

“…The antibacterial activity of electrospun scaffolds against E. coli , S. aureus , and C. albicans was studied by our previous method 30 . In brief, foremost, the concentration of E. coli , S. aureus , and C. albicans solution was diluted repeatedly to reach 10 8 –10 9 CFU/ml, and reached 1.5 × 10 5 –3.5 × 10 5 CFU/ml by four‐step dilution method.…”

“…The antibacterial activity of electrospun scaffolds against E. coli, S. aureus, and C. albicans was studied by our previous method. 30 In brief, foremost, the concentration of E. coli, S. aureus, and C. albicans solution was diluted repeatedly to reach 10 8 -10 9 CFU/ml, and reached 1.5 Â 10 5 -3.5 Â 10 5 CFU/ml by four-step dilution method. Then, 0.1 g sample, 14 ml PBS and 0.1 ml standard bacterial solution was added to 50 ml centrifuge tube and cultured at 37 C for 18 h with oscillator.…”

Fabrication of antimicrobial poly(lactic‐co‐glycolic acid)/silk fibroin/aloe anthraquinone fibrous membranes for potential application of wound healing

“…Electrospun fibrous membranes generally have high porosity, which facilitates air permeability for application in wound healing. 19 Air permeability can be reflected by the water vapor transmission rate (WVTR). PLGA, PLGA/SF, PLGA/AA, and PLGA/SF/AA fibrous membrane have WVTR of 23,684 ± 1128, 25,563 ± 1302, 24,060 ± 1302, 24,812 ± 0 g m À2 day À1 , showing no significant difference between control and indicating their good air permeability (Table 3).…”

“…However, antibiotics generally for therapy in clinic inevitably induce drug resistance, while the widely used metal ions cause the environmental problem and toxicity. 19 Therefore, natural antibacterial agents attract increasing attention in recent years. Animal antimicrobial peptides are loaded on electrospun nanofibrous membranes for fabricating antibacterial wound dressing through direct mixed spinning method or post-processing crosslinking method.…”

“…1,6 Manuka honey-loaded SF composite fibrous mat exhibits good antibacterial activity and excellent biocompatibility, proving its good potential for application in wound healing. 20 A plant extract artemisinin (ART) is used to prepare PLGA/SF/ART composite membrane together with PLGA and SF by electrospinning. Owing to the good antibacterial activity and anti-inflammatory property of ART, this electrospun membrane shows favorable antiinflammatory and cell compatibility in vitro and in vivo evaluation.…”