Silk fibroin nanoparticles for celecoxib and curcumin delivery: ROS-scavenging and anti-inflammatory activities in an in vitro model of osteoarthritis

Crivelli, Barbara; Bari, Elia; Perteghella, Sara; Catenacci, Laura; Sorrenti, Milena; Mocchi, Michela; Faragò, Silvio; Prina-Mello, Adriele; Torre, Maria Luisa

doi:10.1016/j.ejpb.2019.02.008

Cited by 111 publications

(57 citation statements)

References 49 publications

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“…Among these, synthetic or natural nanocarriers, such as solid lipid nanoparticles [5], polymer-nanoparticles [6] and inorganic nanoparticles [7], have been studied. The use of nanoparticles has increased in recent years, due to their unique properties, including their small size and the possibility of targeting cells or tissues, which makes them promising delivery systems for increasing the bioavailability, efficacy and solubility of drugs [8][9][10]. The physical characterization of nanoparticles is a key factor for evaluating their potential for applications for which they have been designed because it is not only their chemical composition that affects their ability to act as successful nanocarriers but also their structure, size distribution and electrostatic properties.As regards NAR, many approaches have been tried, including the use of β-cyclodextrin inclusion complexes [11], phospholipid complexes [12] and polymeric nanoparticles [10].…”

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confidence: 99%

“…In comparison to encapsulation, the adsorption technique has the advantage of being mild, straightforward and cheap (less equipment and fewer materials are required). However, the extent to which drugs can be loaded is entirely dependent on the complex nature of the interactions between the SF and the drug, so that each particular case must be examined.Many studies have confirmed the potential of silk fibroin nanoparticles (SFNs) for capturing and releasing herbal and model drugs [8,9,20,21]. The study presented here consists of a straightforward method which involves mixing the drug with the pre-formed SFNs to see whether the two can physically adsorb to one another through natural intermolecular interactions.…”

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confidence: 99%

“…Many studies have confirmed the potential of silk fibroin nanoparticles (SFNs) for capturing and releasing herbal and model drugs [8,9,20,21]. The study presented here consists of a straightforward method which involves mixing the drug with the pre-formed SFNs to see whether the two can physically adsorb to one another through natural intermolecular interactions.…”

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Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

et al. 2020

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Naringenin (NAR), a flavonoid present in a variety of fruits, vegetables and herbs, exhibits a wide range of pharmacological effects, including anticancer activity. Nevertheless, its application in cancer therapy is limited due to its low bioavailability at the tumour site because of its poor solubility in water and slow dissolution rate. To improve the therapeutic efficacy of NAR, emergent research is looking into using nanocarriers. Silk fibroin (SF), from the Bombyx mori silkworm, is a biocompatible and biodegradable polymer with excellent mechanical properties and an amphiphilic chemistry that make it a promising candidate as a controlled release drug system. The aim of this work is to synthesize naringenin-loaded silk fibroin nanoparticles (NAR-SFNs) by dissolving the SF in the ionic liquid 1-ethyl-3-methylimidazolium acetate, using high-power ultrasounds and rapid desolvation in methanol followed by the adsorption of NAR. The NAR-SFNs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy and thermogravimetric analysis. The drug loading content and encapsulation efficiency were calculated. The drug release profile best fitted a first order equation. The cytotoxicity effects of free NAR, bare silk fibroin nanoparticles (SFNs) and NAR-SFNs were assessed on HeLa and EA.hy926 cells via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated the higher in vitro anticancer potential of synthesized NAR-SFNs than that of free NAR in HeLa cancer cells.

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Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

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“…Over 50 days, 23% FITC-BSA and 34% rhodamine B were released from the SFNPs and internalized by cells, as seen by microscopy and flow cytometry. Crivelli et al synthesized a SFNP using a desolvation technique to encapsulate the anti-inflammatory drugs celecoxib (CXB) or curcumin for osteoarthritis (OA) treatment [ 78 ]. The release of the drug was controlled by varying the drug loading into the SFNP.…”

Section: Applications In Drug Deliverymentioning

confidence: 99%

Recent trends in protein and peptide-based biomaterials for advanced drug delivery

Varanko

Saha

Chilkoti

2020

Advanced Drug Delivery Reviews

213

120

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Engineering protein and peptide-based materials for drug delivery applications has gained momentum due to their biochemical and biophysical properties over synthetic materials, including biocompatibility, ease of synthesis and purification, tunability, scalability, and lack of toxicity. These biomolecules have been used to develop a host of drug delivery platforms, such as peptide- and protein-drug conjugates, injectable particles, and drug depots to deliver small molecule drugs, therapeutic proteins, and nucleic acids. In this review, we discuss progress in engineering the architecture and biological functions of peptide-based biomaterials —naturally derived, chemically synthesized and recombinant— with a focus on the molecular features that modulate their structure-function relationships for drug delivery.

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“…Similarly, curcumin is shown to reduce in ammatory responses by regulating NF-κB-related signaling pathways in a rat model of sepsis with acute lung injury (Shaikh SB, 2019). Anti-in ammatory, anti-oxidative and anti-apoptotic effects of curcumin have also been con rmed in studies on osteoarthritis (Crivelli B, 2019). However, effects of curcumin in improving tendinopathy and related mechanisms have been rarely reported to date.…”

Section: Introductionmentioning

confidence: 99%

Curcumin Improve Tendinopathy via Regulation TLR4 Pathway in Vitro and Vivo Study

Tang¹,

Guo²,

Xu³

2020

Preprint

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BackgroundTendon disorders are a common type of soft tissue disease mainly characterized by various tendon syndromes with chronic pain. The purpose of this study was to evaluate curcumin had effects to improve tendon disorders by vitro and vivo study.ResultsCurcumin shows pharmacological activities similar to that of traditional anti-inflammatory agents and favorable therapeutic effects on various chronic inflammation-related diseases. In our present study, we discussed curcumin had effects to improve tendinopathy by vitro and vivo study. In further research, the study clearly explained the mechanisms by vitro study. The results were shown that curcumin had effects to improved tendinopathy which induced by TLR4/NF-κB(p65) pathway activating in vivo and vitro experiments. However, with TLR4 supplement, the treatment effect of curcumin has been disappear. ConclusionCurcumin had effects to improve tedinopathy via regulation depressing TLR4/NF-κB(p65) pathway and suppressing p-NF-κB(p65) nuclear volume.

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Silk fibroin nanoparticles for celecoxib and curcumin delivery: ROS-scavenging and anti-inflammatory activities in an in vitro model of osteoarthritis

Cited by 111 publications

References 49 publications

Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

Recent trends in protein and peptide-based biomaterials for advanced drug delivery

Curcumin Improve Tendinopathy via Regulation TLR4 Pathway in Vitro and Vivo Study

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